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Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy
[Image: see text] Herein, we present a water-soluble dendritric Pt(II) complex as a phosphorescent label for bacterial cells. The dendritic moiety endows the Pt(II) complex with unique properties such as water solubility, shielding from quenching by dioxygen, and binding to bacterial surfaces. The n...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704438/ https://www.ncbi.nlm.nih.gov/pubmed/31460429 http://dx.doi.org/10.1021/acsomega.9b00639 |
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author | Molina, Noemi Cnudde, Marvin Guadix, Juan A. Perez-Pomares, Jose M. Strassert, Cristian A. Vida, Yolanda Perez-Inestrosa, Ezequiel |
author_facet | Molina, Noemi Cnudde, Marvin Guadix, Juan A. Perez-Pomares, Jose M. Strassert, Cristian A. Vida, Yolanda Perez-Inestrosa, Ezequiel |
author_sort | Molina, Noemi |
collection | PubMed |
description | [Image: see text] Herein, we present a water-soluble dendritric Pt(II) complex as a phosphorescent label for bacterial cells. The dendritic moiety endows the Pt(II) complex with unique properties such as water solubility, shielding from quenching by dioxygen, and binding to bacterial surfaces. The new biosensor was employed for two-photon excitation microscopy, and the binding was confirmed by electron microscopy, which demonstrates that such hybrid arrays can provide orthogonal yet complementary readouts. |
format | Online Article Text |
id | pubmed-6704438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-67044382019-08-27 Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy Molina, Noemi Cnudde, Marvin Guadix, Juan A. Perez-Pomares, Jose M. Strassert, Cristian A. Vida, Yolanda Perez-Inestrosa, Ezequiel ACS Omega [Image: see text] Herein, we present a water-soluble dendritric Pt(II) complex as a phosphorescent label for bacterial cells. The dendritic moiety endows the Pt(II) complex with unique properties such as water solubility, shielding from quenching by dioxygen, and binding to bacterial surfaces. The new biosensor was employed for two-photon excitation microscopy, and the binding was confirmed by electron microscopy, which demonstrates that such hybrid arrays can provide orthogonal yet complementary readouts. American Chemical Society 2019-08-06 /pmc/articles/PMC6704438/ /pubmed/31460429 http://dx.doi.org/10.1021/acsomega.9b00639 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Molina, Noemi Cnudde, Marvin Guadix, Juan A. Perez-Pomares, Jose M. Strassert, Cristian A. Vida, Yolanda Perez-Inestrosa, Ezequiel Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy |
title | Platinum-Doped Dendritic Structure as a Phosphorescent
Label for Bacteria in Two-Photon
Excitation Microscopy |
title_full | Platinum-Doped Dendritic Structure as a Phosphorescent
Label for Bacteria in Two-Photon
Excitation Microscopy |
title_fullStr | Platinum-Doped Dendritic Structure as a Phosphorescent
Label for Bacteria in Two-Photon
Excitation Microscopy |
title_full_unstemmed | Platinum-Doped Dendritic Structure as a Phosphorescent
Label for Bacteria in Two-Photon
Excitation Microscopy |
title_short | Platinum-Doped Dendritic Structure as a Phosphorescent
Label for Bacteria in Two-Photon
Excitation Microscopy |
title_sort | platinum-doped dendritic structure as a phosphorescent
label for bacteria in two-photon
excitation microscopy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704438/ https://www.ncbi.nlm.nih.gov/pubmed/31460429 http://dx.doi.org/10.1021/acsomega.9b00639 |
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