Cargando…

Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy

[Image: see text] Herein, we present a water-soluble dendritric Pt(II) complex as a phosphorescent label for bacterial cells. The dendritic moiety endows the Pt(II) complex with unique properties such as water solubility, shielding from quenching by dioxygen, and binding to bacterial surfaces. The n...

Descripción completa

Detalles Bibliográficos
Autores principales: Molina, Noemi, Cnudde, Marvin, Guadix, Juan A., Perez-Pomares, Jose M., Strassert, Cristian A., Vida, Yolanda, Perez-Inestrosa, Ezequiel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704438/
https://www.ncbi.nlm.nih.gov/pubmed/31460429
http://dx.doi.org/10.1021/acsomega.9b00639
_version_ 1783445506683305984
author Molina, Noemi
Cnudde, Marvin
Guadix, Juan A.
Perez-Pomares, Jose M.
Strassert, Cristian A.
Vida, Yolanda
Perez-Inestrosa, Ezequiel
author_facet Molina, Noemi
Cnudde, Marvin
Guadix, Juan A.
Perez-Pomares, Jose M.
Strassert, Cristian A.
Vida, Yolanda
Perez-Inestrosa, Ezequiel
author_sort Molina, Noemi
collection PubMed
description [Image: see text] Herein, we present a water-soluble dendritric Pt(II) complex as a phosphorescent label for bacterial cells. The dendritic moiety endows the Pt(II) complex with unique properties such as water solubility, shielding from quenching by dioxygen, and binding to bacterial surfaces. The new biosensor was employed for two-photon excitation microscopy, and the binding was confirmed by electron microscopy, which demonstrates that such hybrid arrays can provide orthogonal yet complementary readouts.
format Online
Article
Text
id pubmed-6704438
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-67044382019-08-27 Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy Molina, Noemi Cnudde, Marvin Guadix, Juan A. Perez-Pomares, Jose M. Strassert, Cristian A. Vida, Yolanda Perez-Inestrosa, Ezequiel ACS Omega [Image: see text] Herein, we present a water-soluble dendritric Pt(II) complex as a phosphorescent label for bacterial cells. The dendritic moiety endows the Pt(II) complex with unique properties such as water solubility, shielding from quenching by dioxygen, and binding to bacterial surfaces. The new biosensor was employed for two-photon excitation microscopy, and the binding was confirmed by electron microscopy, which demonstrates that such hybrid arrays can provide orthogonal yet complementary readouts. American Chemical Society 2019-08-06 /pmc/articles/PMC6704438/ /pubmed/31460429 http://dx.doi.org/10.1021/acsomega.9b00639 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Molina, Noemi
Cnudde, Marvin
Guadix, Juan A.
Perez-Pomares, Jose M.
Strassert, Cristian A.
Vida, Yolanda
Perez-Inestrosa, Ezequiel
Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy
title Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy
title_full Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy
title_fullStr Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy
title_full_unstemmed Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy
title_short Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy
title_sort platinum-doped dendritic structure as a phosphorescent label for bacteria in two-photon excitation microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704438/
https://www.ncbi.nlm.nih.gov/pubmed/31460429
http://dx.doi.org/10.1021/acsomega.9b00639
work_keys_str_mv AT molinanoemi platinumdopeddendriticstructureasaphosphorescentlabelforbacteriaintwophotonexcitationmicroscopy
AT cnuddemarvin platinumdopeddendriticstructureasaphosphorescentlabelforbacteriaintwophotonexcitationmicroscopy
AT guadixjuana platinumdopeddendriticstructureasaphosphorescentlabelforbacteriaintwophotonexcitationmicroscopy
AT perezpomaresjosem platinumdopeddendriticstructureasaphosphorescentlabelforbacteriaintwophotonexcitationmicroscopy
AT strassertcristiana platinumdopeddendriticstructureasaphosphorescentlabelforbacteriaintwophotonexcitationmicroscopy
AT vidayolanda platinumdopeddendriticstructureasaphosphorescentlabelforbacteriaintwophotonexcitationmicroscopy
AT perezinestrosaezequiel platinumdopeddendriticstructureasaphosphorescentlabelforbacteriaintwophotonexcitationmicroscopy