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Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development

For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island...

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Autores principales: Hu, Wen-Xing, Kim, Byoung-Jo, Kwak, Younghwan, Seo, Eun-Young, Kim, Jung-Kyu, Han, Jae-Yeong, Kim, Ik-Hyun, Lim, Yong Pyo, Cho, In-Sook, Domier, Leslie L, Hammond, John, Lim, Hyoun-Sub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706019/
https://www.ncbi.nlm.nih.gov/pubmed/31481861
http://dx.doi.org/10.5423/PPJ.NT.11.2018.0238
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author Hu, Wen-Xing
Kim, Byoung-Jo
Kwak, Younghwan
Seo, Eun-Young
Kim, Jung-Kyu
Han, Jae-Yeong
Kim, Ik-Hyun
Lim, Yong Pyo
Cho, In-Sook
Domier, Leslie L
Hammond, John
Lim, Hyoun-Sub
author_facet Hu, Wen-Xing
Kim, Byoung-Jo
Kwak, Younghwan
Seo, Eun-Young
Kim, Jung-Kyu
Han, Jae-Yeong
Kim, Ik-Hyun
Lim, Yong Pyo
Cho, In-Sook
Domier, Leslie L
Hammond, John
Lim, Hyoun-Sub
author_sort Hu, Wen-Xing
collection PubMed
description For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey on Jeju Island in 2017. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bacteriophage T7 RNA polymerase promoters. Their fulllength sequences share 98.9–99.9% nucleotide sequence identity and were most closely related to previously reported Korean TuMV isolates. All isolates belonged to the BR group and infected both Chinese cabbage and radish. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom differences may result from three amino acid differences uniquely present in KBJ5; Gly(382)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively.
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spelling pubmed-67060192019-09-03 Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development Hu, Wen-Xing Kim, Byoung-Jo Kwak, Younghwan Seo, Eun-Young Kim, Jung-Kyu Han, Jae-Yeong Kim, Ik-Hyun Lim, Yong Pyo Cho, In-Sook Domier, Leslie L Hammond, John Lim, Hyoun-Sub Plant Pathol J Note For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey on Jeju Island in 2017. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bacteriophage T7 RNA polymerase promoters. Their fulllength sequences share 98.9–99.9% nucleotide sequence identity and were most closely related to previously reported Korean TuMV isolates. All isolates belonged to the BR group and infected both Chinese cabbage and radish. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom differences may result from three amino acid differences uniquely present in KBJ5; Gly(382)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively. Korean Society of Plant Pathology 2019-08 2019-08-01 /pmc/articles/PMC6706019/ /pubmed/31481861 http://dx.doi.org/10.5423/PPJ.NT.11.2018.0238 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Hu, Wen-Xing
Kim, Byoung-Jo
Kwak, Younghwan
Seo, Eun-Young
Kim, Jung-Kyu
Han, Jae-Yeong
Kim, Ik-Hyun
Lim, Yong Pyo
Cho, In-Sook
Domier, Leslie L
Hammond, John
Lim, Hyoun-Sub
Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development
title Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development
title_full Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development
title_fullStr Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development
title_full_unstemmed Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development
title_short Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development
title_sort five newly collected turnip mosaic virus (tumv) isolates from jeju island, korea are closely related to previously reported korean tumv isolates but show distinctive symptom development
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706019/
https://www.ncbi.nlm.nih.gov/pubmed/31481861
http://dx.doi.org/10.5423/PPJ.NT.11.2018.0238
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