Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method

This study demonstrated that bead-beating method facilitates a simple and rapid protocol for genomic DNA isolation for Pacific BioSciences (PacBio) sequencing with library construction of sufficient length. The protocol may also be beneficial for inactivating pathogens by simultaneous and instant DN...

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Detalles Bibliográficos
Autores principales: Kato, Kengo, Hashino, Masanori, Ito, Tamaki, Matsui, Mari, Suzuki, Satowa, Kai, Kumiko, Kitazume, Miwako, Sekizuka, Tsuyoshi, Kuroda, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Biological Methods 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706104/
https://www.ncbi.nlm.nih.gov/pubmed/31453233
http://dx.doi.org/10.14440/jbm.2017.169
Descripción
Sumario:This study demonstrated that bead-beating method facilitates a simple and rapid protocol for genomic DNA isolation for Pacific BioSciences (PacBio) sequencing with library construction of sufficient length. The protocol may also be beneficial for inactivating pathogens by simultaneous and instant DNA fragmentation, with no special equipment required to obtain large DNA fragments. This protocol was comparable in terms of quality to the standard protocol suggested by PacBio and represents an alternative, rapid shortcut for performing accurate PacBio sequencing.

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