Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method

This study demonstrated that bead-beating method facilitates a simple and rapid protocol for genomic DNA isolation for Pacific BioSciences (PacBio) sequencing with library construction of sufficient length. The protocol may also be beneficial for inactivating pathogens by simultaneous and instant DN...

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Autores principales: Kato, Kengo, Hashino, Masanori, Ito, Tamaki, Matsui, Mari, Suzuki, Satowa, Kai, Kumiko, Kitazume, Miwako, Sekizuka, Tsuyoshi, Kuroda, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Biological Methods 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706104/
https://www.ncbi.nlm.nih.gov/pubmed/31453233
http://dx.doi.org/10.14440/jbm.2017.169
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author Kato, Kengo
Hashino, Masanori
Ito, Tamaki
Matsui, Mari
Suzuki, Satowa
Kai, Kumiko
Kitazume, Miwako
Sekizuka, Tsuyoshi
Kuroda, Makoto
author_facet Kato, Kengo
Hashino, Masanori
Ito, Tamaki
Matsui, Mari
Suzuki, Satowa
Kai, Kumiko
Kitazume, Miwako
Sekizuka, Tsuyoshi
Kuroda, Makoto
author_sort Kato, Kengo
collection PubMed
description This study demonstrated that bead-beating method facilitates a simple and rapid protocol for genomic DNA isolation for Pacific BioSciences (PacBio) sequencing with library construction of sufficient length. The protocol may also be beneficial for inactivating pathogens by simultaneous and instant DNA fragmentation, with no special equipment required to obtain large DNA fragments. This protocol was comparable in terms of quality to the standard protocol suggested by PacBio and represents an alternative, rapid shortcut for performing accurate PacBio sequencing.
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spelling pubmed-67061042019-08-26 Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method Kato, Kengo Hashino, Masanori Ito, Tamaki Matsui, Mari Suzuki, Satowa Kai, Kumiko Kitazume, Miwako Sekizuka, Tsuyoshi Kuroda, Makoto J Biol Methods Article This study demonstrated that bead-beating method facilitates a simple and rapid protocol for genomic DNA isolation for Pacific BioSciences (PacBio) sequencing with library construction of sufficient length. The protocol may also be beneficial for inactivating pathogens by simultaneous and instant DNA fragmentation, with no special equipment required to obtain large DNA fragments. This protocol was comparable in terms of quality to the standard protocol suggested by PacBio and represents an alternative, rapid shortcut for performing accurate PacBio sequencing. Journal of Biological Methods 2017-09-13 /pmc/articles/PMC6706104/ /pubmed/31453233 http://dx.doi.org/10.14440/jbm.2017.169 Text en © 2013-2018 The Journal of Biological Methods, All rights reserved. https://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License.
spellingShingle Article
Kato, Kengo
Hashino, Masanori
Ito, Tamaki
Matsui, Mari
Suzuki, Satowa
Kai, Kumiko
Kitazume, Miwako
Sekizuka, Tsuyoshi
Kuroda, Makoto
Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
title Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
title_full Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
title_fullStr Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
title_full_unstemmed Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
title_short Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
title_sort rapid and affordable size-selected pacbio single-molecule real-time sequencing template library construction using the bead-beating dna extraction method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706104/
https://www.ncbi.nlm.nih.gov/pubmed/31453233
http://dx.doi.org/10.14440/jbm.2017.169
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