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Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
This study demonstrated that bead-beating method facilitates a simple and rapid protocol for genomic DNA isolation for Pacific BioSciences (PacBio) sequencing with library construction of sufficient length. The protocol may also be beneficial for inactivating pathogens by simultaneous and instant DN...
Autores principales: | Kato, Kengo, Hashino, Masanori, Ito, Tamaki, Matsui, Mari, Suzuki, Satowa, Kai, Kumiko, Kitazume, Miwako, Sekizuka, Tsuyoshi, Kuroda, Makoto |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Journal of Biological Methods
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706104/ https://www.ncbi.nlm.nih.gov/pubmed/31453233 http://dx.doi.org/10.14440/jbm.2017.169 |
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