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Measurement of intracellular calcium of submandibular glands using a high throughput plate reader

Calcium ions (Ca(2+)) impact nearly every aspect of cellular life and intracellular calcium [Ca(2+)](i) is a critical factor in the regulation of a plethora of physiological functions, including: muscle contraction, saliva secretion, metabolism, gene expression, cell survival and death. By measuring...

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Detalles Bibliográficos
Autores principales: Shaalan, Abeer Kamal, Carpenter, Guy, Proctor, Gordon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Biological Methods 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706108/
https://www.ncbi.nlm.nih.gov/pubmed/31453231
http://dx.doi.org/10.14440/jbm.2017.180
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author Shaalan, Abeer Kamal
Carpenter, Guy
Proctor, Gordon
author_facet Shaalan, Abeer Kamal
Carpenter, Guy
Proctor, Gordon
author_sort Shaalan, Abeer Kamal
collection PubMed
description Calcium ions (Ca(2+)) impact nearly every aspect of cellular life and intracellular calcium [Ca(2+)](i) is a critical factor in the regulation of a plethora of physiological functions, including: muscle contraction, saliva secretion, metabolism, gene expression, cell survival and death. By measuring the changes of [Ca(2+)](i) levels, critical physiologic functions can be characterized and aberrant pathologic conditions or drug responses can be efficiently monitored. We developed a protocol for assessment of Ca(2+) signaling in the acinar units of submandibular glands isolated from C57BL/6 mice, using benchtop, multi-mode, high throughput plate reader (FlexStation 3). This method represents a powerful tool for unlimited in vitro studies to monitor changes in receptor-mediated Ca(2+) responses while retaining functional and morphological features of a native setting.
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spelling pubmed-67061082019-08-26 Measurement of intracellular calcium of submandibular glands using a high throughput plate reader Shaalan, Abeer Kamal Carpenter, Guy Proctor, Gordon J Biol Methods Protocol Calcium ions (Ca(2+)) impact nearly every aspect of cellular life and intracellular calcium [Ca(2+)](i) is a critical factor in the regulation of a plethora of physiological functions, including: muscle contraction, saliva secretion, metabolism, gene expression, cell survival and death. By measuring the changes of [Ca(2+)](i) levels, critical physiologic functions can be characterized and aberrant pathologic conditions or drug responses can be efficiently monitored. We developed a protocol for assessment of Ca(2+) signaling in the acinar units of submandibular glands isolated from C57BL/6 mice, using benchtop, multi-mode, high throughput plate reader (FlexStation 3). This method represents a powerful tool for unlimited in vitro studies to monitor changes in receptor-mediated Ca(2+) responses while retaining functional and morphological features of a native setting. Journal of Biological Methods 2017-07-17 /pmc/articles/PMC6706108/ /pubmed/31453231 http://dx.doi.org/10.14440/jbm.2017.180 Text en © 2013-2018 The Journal of Biological Methods, All rights reserved. https://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License.
spellingShingle Protocol
Shaalan, Abeer Kamal
Carpenter, Guy
Proctor, Gordon
Measurement of intracellular calcium of submandibular glands using a high throughput plate reader
title Measurement of intracellular calcium of submandibular glands using a high throughput plate reader
title_full Measurement of intracellular calcium of submandibular glands using a high throughput plate reader
title_fullStr Measurement of intracellular calcium of submandibular glands using a high throughput plate reader
title_full_unstemmed Measurement of intracellular calcium of submandibular glands using a high throughput plate reader
title_short Measurement of intracellular calcium of submandibular glands using a high throughput plate reader
title_sort measurement of intracellular calcium of submandibular glands using a high throughput plate reader
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706108/
https://www.ncbi.nlm.nih.gov/pubmed/31453231
http://dx.doi.org/10.14440/jbm.2017.180
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