Easy and low-cost stable positioning of suspension cells during live-cell imaging

Dynamic processes of cells can be best monitored when living cells are analyzed by imaging. While it is easy to observe adherent living cells it has been extremely challenging to analyze suspension cells. This cell type floats freely in the culture dish, and it is only a question of time when the fo...

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Detalles Bibliográficos
Autores principales: Ivanusic, Daniel, Madela, Kazimierz, Denner, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Biological Methods 2017
Materias:
Benchmark
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706139/
https://www.ncbi.nlm.nih.gov/pubmed/31453234
http://dx.doi.org/10.14440/jbm.2017.203
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author Ivanusic, Daniel
Madela, Kazimierz
Denner, Joachim
author_facet Ivanusic, Daniel
Madela, Kazimierz
Denner, Joachim
author_sort Ivanusic, Daniel
collection PubMed
description Dynamic processes of cells can be best monitored when living cells are analyzed by imaging. While it is easy to observe adherent living cells it has been extremely challenging to analyze suspension cells. This cell type floats freely in the culture dish, and it is only a question of time when the focus or the observation field is lost. In order to keep the cells in focus, an easy and inexpensive method allowing the observation of living suspension cells during confocal laser scanning microscope imaging was developed.
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spelling pubmed-67061392019-08-26 Easy and low-cost stable positioning of suspension cells during live-cell imaging Ivanusic, Daniel Madela, Kazimierz Denner, Joachim J Biol Methods Benchmark Dynamic processes of cells can be best monitored when living cells are analyzed by imaging. While it is easy to observe adherent living cells it has been extremely challenging to analyze suspension cells. This cell type floats freely in the culture dish, and it is only a question of time when the focus or the observation field is lost. In order to keep the cells in focus, an easy and inexpensive method allowing the observation of living suspension cells during confocal laser scanning microscope imaging was developed. Journal of Biological Methods 2017-10-17 /pmc/articles/PMC6706139/ /pubmed/31453234 http://dx.doi.org/10.14440/jbm.2017.203 Text en © 2013-2018 The Journal of Biological Methods, All rights reserved. https://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License.
spellingShingle Benchmark
Ivanusic, Daniel
Madela, Kazimierz
Denner, Joachim
Easy and low-cost stable positioning of suspension cells during live-cell imaging
title Easy and low-cost stable positioning of suspension cells during live-cell imaging
title_full Easy and low-cost stable positioning of suspension cells during live-cell imaging
title_fullStr Easy and low-cost stable positioning of suspension cells during live-cell imaging
title_full_unstemmed Easy and low-cost stable positioning of suspension cells during live-cell imaging
title_short Easy and low-cost stable positioning of suspension cells during live-cell imaging
title_sort easy and low-cost stable positioning of suspension cells during live-cell imaging
topic Benchmark
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706139/
https://www.ncbi.nlm.nih.gov/pubmed/31453234
http://dx.doi.org/10.14440/jbm.2017.203
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