Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa

This study designed to assess the expression level of CATSPER2 and TEKT2 and to evaluate the levels of CatSper2 and Tektin2 proteins in human spermatozoa before and after cryopreservation. One hundred and twenty semen samples were included in this study. All the samples were subjected to qPCR and We...

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Detalles Bibliográficos
Autores principales: Alshawa, Eiman, Laqqan, Mohammed, Montenarh, Mathias, Hammadeh, Mohamad Eid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706526/
https://www.ncbi.nlm.nih.gov/pubmed/31463202
http://dx.doi.org/10.1016/j.toxrep.2019.08.004
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author Alshawa, Eiman
Laqqan, Mohammed
Montenarh, Mathias
Hammadeh, Mohamad Eid
author_facet Alshawa, Eiman
Laqqan, Mohammed
Montenarh, Mathias
Hammadeh, Mohamad Eid
author_sort Alshawa, Eiman
collection PubMed
description This study designed to assess the expression level of CATSPER2 and TEKT2 and to evaluate the levels of CatSper2 and Tektin2 proteins in human spermatozoa before and after cryopreservation. One hundred and twenty semen samples were included in this study. All the samples were subjected to qPCR and Western blot analysis. The results showed a significant reduction in the expression levels of CATSPER2 and TEKT2 in the cryopreserved compared to the fresh samples (P = 0.0039 and P = 0.0166, respectively), and the results showed down-regulation in the expression level of CATSPER2 and TEKT2 genes between the study groups. Moreover, the protein levels of the CatSper2 and Tektin2 were lower in cryopreserved samples compared to fresh samples (P = 0.0001). In conclusion, the reduction in the proteins level and expression level of the CATSPER2 and TEKT2 in cryopreserved samples could be used as an indicator of sperm motility loss.
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spelling pubmed-67065262019-08-28 Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa Alshawa, Eiman Laqqan, Mohammed Montenarh, Mathias Hammadeh, Mohamad Eid Toxicol Rep Article This study designed to assess the expression level of CATSPER2 and TEKT2 and to evaluate the levels of CatSper2 and Tektin2 proteins in human spermatozoa before and after cryopreservation. One hundred and twenty semen samples were included in this study. All the samples were subjected to qPCR and Western blot analysis. The results showed a significant reduction in the expression levels of CATSPER2 and TEKT2 in the cryopreserved compared to the fresh samples (P = 0.0039 and P = 0.0166, respectively), and the results showed down-regulation in the expression level of CATSPER2 and TEKT2 genes between the study groups. Moreover, the protein levels of the CatSper2 and Tektin2 were lower in cryopreserved samples compared to fresh samples (P = 0.0001). In conclusion, the reduction in the proteins level and expression level of the CATSPER2 and TEKT2 in cryopreserved samples could be used as an indicator of sperm motility loss. Elsevier 2019-08-06 /pmc/articles/PMC6706526/ /pubmed/31463202 http://dx.doi.org/10.1016/j.toxrep.2019.08.004 Text en © 2019 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Alshawa, Eiman
Laqqan, Mohammed
Montenarh, Mathias
Hammadeh, Mohamad Eid
Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_full Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_fullStr Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_full_unstemmed Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_short Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_sort influence of cryopreservation on the catsper2 and tekt2 expression levels and protein levels in human spermatozoa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706526/
https://www.ncbi.nlm.nih.gov/pubmed/31463202
http://dx.doi.org/10.1016/j.toxrep.2019.08.004
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