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Determination of Two Antiepileptic Drugs in Urine by Homogenous Liquid-Liquid Extraction Performed in A Narrow Tube Combined with Dispersive Liquid-liquid Microextraction Followed by Gas Chromatography-flame Ionization Detection
A simple and efficient homogenous liquid-liquid extraction method performed in a narrow tube combined with dispersive liquid-liquid microextraction method has been presented for the simultaneous determination of two antiepileptic drugs in urine followed by gas chromatography with flame ionization de...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706726/ https://www.ncbi.nlm.nih.gov/pubmed/31531046 http://dx.doi.org/10.22037/ijpr.2019.1100635 |
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author | Feriduni, Behruz Farajzadeh, Mir Ali Jouyban, Abolghasem |
author_facet | Feriduni, Behruz Farajzadeh, Mir Ali Jouyban, Abolghasem |
author_sort | Feriduni, Behruz |
collection | PubMed |
description | A simple and efficient homogenous liquid-liquid extraction method performed in a narrow tube combined with dispersive liquid-liquid microextraction method has been presented for the simultaneous determination of two antiepileptic drugs in urine followed by gas chromatography with flame ionization detection. In this method, a mixture of acetonitrile and urine sample (homogenous solution) is loaded into a column partially filled with solid sodium chloride. By passing the homogenous solution through the salt layer, acetonitrile is separated from the aqueous solution as the fine droplets and collected on top of the column as a separated phase. The obtained organic phase is removed and mixed with an extraction solvent, and then the resulting mixture is rapidly injected into an alkaline solution. Various experimental parameters affecting performance of the proposed method such as type and volume of extraction solvent, pH, and flow rate in homogenous liquid-liquid extraction step, and type and volume of extraction solvent and ionic strength in dispersive liquid-liquid microextraction step were investigated. The relative standard deviation of the proposed method was <8% (n = 6, C = 1 µg L(-1) of each analyte). The limits of detection for phenobarbital and carbamazepine were 0.017 and 0.010 µg L(-1) and the limits of quantification were 0.056 and 0.033 µg mL-(1), respectively. |
format | Online Article Text |
id | pubmed-6706726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-67067262019-09-17 Determination of Two Antiepileptic Drugs in Urine by Homogenous Liquid-Liquid Extraction Performed in A Narrow Tube Combined with Dispersive Liquid-liquid Microextraction Followed by Gas Chromatography-flame Ionization Detection Feriduni, Behruz Farajzadeh, Mir Ali Jouyban, Abolghasem Iran J Pharm Res Original Article A simple and efficient homogenous liquid-liquid extraction method performed in a narrow tube combined with dispersive liquid-liquid microextraction method has been presented for the simultaneous determination of two antiepileptic drugs in urine followed by gas chromatography with flame ionization detection. In this method, a mixture of acetonitrile and urine sample (homogenous solution) is loaded into a column partially filled with solid sodium chloride. By passing the homogenous solution through the salt layer, acetonitrile is separated from the aqueous solution as the fine droplets and collected on top of the column as a separated phase. The obtained organic phase is removed and mixed with an extraction solvent, and then the resulting mixture is rapidly injected into an alkaline solution. Various experimental parameters affecting performance of the proposed method such as type and volume of extraction solvent, pH, and flow rate in homogenous liquid-liquid extraction step, and type and volume of extraction solvent and ionic strength in dispersive liquid-liquid microextraction step were investigated. The relative standard deviation of the proposed method was <8% (n = 6, C = 1 µg L(-1) of each analyte). The limits of detection for phenobarbital and carbamazepine were 0.017 and 0.010 µg L(-1) and the limits of quantification were 0.056 and 0.033 µg mL-(1), respectively. Shaheed Beheshti University of Medical Sciences 2019 /pmc/articles/PMC6706726/ /pubmed/31531046 http://dx.doi.org/10.22037/ijpr.2019.1100635 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Feriduni, Behruz Farajzadeh, Mir Ali Jouyban, Abolghasem Determination of Two Antiepileptic Drugs in Urine by Homogenous Liquid-Liquid Extraction Performed in A Narrow Tube Combined with Dispersive Liquid-liquid Microextraction Followed by Gas Chromatography-flame Ionization Detection |
title | Determination of Two Antiepileptic Drugs in Urine by Homogenous Liquid-Liquid Extraction Performed in A Narrow Tube Combined with Dispersive Liquid-liquid Microextraction Followed by Gas Chromatography-flame Ionization Detection |
title_full | Determination of Two Antiepileptic Drugs in Urine by Homogenous Liquid-Liquid Extraction Performed in A Narrow Tube Combined with Dispersive Liquid-liquid Microextraction Followed by Gas Chromatography-flame Ionization Detection |
title_fullStr | Determination of Two Antiepileptic Drugs in Urine by Homogenous Liquid-Liquid Extraction Performed in A Narrow Tube Combined with Dispersive Liquid-liquid Microextraction Followed by Gas Chromatography-flame Ionization Detection |
title_full_unstemmed | Determination of Two Antiepileptic Drugs in Urine by Homogenous Liquid-Liquid Extraction Performed in A Narrow Tube Combined with Dispersive Liquid-liquid Microextraction Followed by Gas Chromatography-flame Ionization Detection |
title_short | Determination of Two Antiepileptic Drugs in Urine by Homogenous Liquid-Liquid Extraction Performed in A Narrow Tube Combined with Dispersive Liquid-liquid Microextraction Followed by Gas Chromatography-flame Ionization Detection |
title_sort | determination of two antiepileptic drugs in urine by homogenous liquid-liquid extraction performed in a narrow tube combined with dispersive liquid-liquid microextraction followed by gas chromatography-flame ionization detection |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706726/ https://www.ncbi.nlm.nih.gov/pubmed/31531046 http://dx.doi.org/10.22037/ijpr.2019.1100635 |
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