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Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA
A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706939/ https://www.ncbi.nlm.nih.gov/pubmed/31463479 http://dx.doi.org/10.1186/s13065-019-0622-7 |
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author | Qi, Lu Zhang, Aihong Wang, Yu Liu, Long Wang, Xinghe |
author_facet | Qi, Lu Zhang, Aihong Wang, Yu Liu, Long Wang, Xinghe |
author_sort | Qi, Lu |
collection | PubMed |
description | A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13065-019-0622-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6706939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-67069392019-08-28 Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA Qi, Lu Zhang, Aihong Wang, Yu Liu, Long Wang, Xinghe BMC Chem Research Article A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13065-019-0622-7) contains supplementary material, which is available to authorized users. Springer International Publishing 2019-08-22 /pmc/articles/PMC6706939/ /pubmed/31463479 http://dx.doi.org/10.1186/s13065-019-0622-7 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Qi, Lu Zhang, Aihong Wang, Yu Liu, Long Wang, Xinghe Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA |
title | Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA |
title_full | Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA |
title_fullStr | Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA |
title_full_unstemmed | Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA |
title_short | Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA |
title_sort | atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based elisa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706939/ https://www.ncbi.nlm.nih.gov/pubmed/31463479 http://dx.doi.org/10.1186/s13065-019-0622-7 |
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