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Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer

BACKGROUND: Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and Helicobacter pylori (H. pylori) is the major risk factor for GC. In this study, we focused on the roles of H. pylori-rela...

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Autores principales: Jia, Wenxiao, Zhang, Jiaqin, Ma, Fang, Hao, Shengjie, Li, Xue, Guo, Ruiting, Gao, Qianqian, Sun, Yundong, Jia, Jihui, Li, Wenjuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707351/
https://www.ncbi.nlm.nih.gov/pubmed/32021238
http://dx.doi.org/10.2147/OTT.S201832
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author Jia, Wenxiao
Zhang, Jiaqin
Ma, Fang
Hao, Shengjie
Li, Xue
Guo, Ruiting
Gao, Qianqian
Sun, Yundong
Jia, Jihui
Li, Wenjuan
author_facet Jia, Wenxiao
Zhang, Jiaqin
Ma, Fang
Hao, Shengjie
Li, Xue
Guo, Ruiting
Gao, Qianqian
Sun, Yundong
Jia, Jihui
Li, Wenjuan
author_sort Jia, Wenxiao
collection PubMed
description BACKGROUND: Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and Helicobacter pylori (H. pylori) is the major risk factor for GC. In this study, we focused on the roles of H. pylori-related lncRNAs in the progression of GC. METHOD: Differentially expressed lncRNAs were identified through RNA-seq analysis of H. pylori-infected GC cells. RESULTS: We found that the expression of the lncRNA THAP9-AS1 was up-regulated after infection of GC cells with H. pylori and was higher in GC tissues than in gastritis tissues. Colony formation, CCK8 and transwell assays were executed to show that THAP9-AS1 can promote GC cell proliferation and migration in vitro. Our study identified the pro-oncogenic lncRNA THAP9-AS1, which has a higher expression level in GC tissues than in gastritis tissues and which promoted the proliferation and migration of GC cells in vitro. CONCLUSION: These findings may provide a potential therapeutic target for H. pylori-associated GC.
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spelling pubmed-67073512020-02-04 Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer Jia, Wenxiao Zhang, Jiaqin Ma, Fang Hao, Shengjie Li, Xue Guo, Ruiting Gao, Qianqian Sun, Yundong Jia, Jihui Li, Wenjuan Onco Targets Ther Original Research BACKGROUND: Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and Helicobacter pylori (H. pylori) is the major risk factor for GC. In this study, we focused on the roles of H. pylori-related lncRNAs in the progression of GC. METHOD: Differentially expressed lncRNAs were identified through RNA-seq analysis of H. pylori-infected GC cells. RESULTS: We found that the expression of the lncRNA THAP9-AS1 was up-regulated after infection of GC cells with H. pylori and was higher in GC tissues than in gastritis tissues. Colony formation, CCK8 and transwell assays were executed to show that THAP9-AS1 can promote GC cell proliferation and migration in vitro. Our study identified the pro-oncogenic lncRNA THAP9-AS1, which has a higher expression level in GC tissues than in gastritis tissues and which promoted the proliferation and migration of GC cells in vitro. CONCLUSION: These findings may provide a potential therapeutic target for H. pylori-associated GC. Dove 2019-08-19 /pmc/articles/PMC6707351/ /pubmed/32021238 http://dx.doi.org/10.2147/OTT.S201832 Text en © 2019 Jia et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Jia, Wenxiao
Zhang, Jiaqin
Ma, Fang
Hao, Shengjie
Li, Xue
Guo, Ruiting
Gao, Qianqian
Sun, Yundong
Jia, Jihui
Li, Wenjuan
Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer
title Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer
title_full Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer
title_fullStr Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer
title_full_unstemmed Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer
title_short Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer
title_sort long noncoding rna thap9-as1 is induced by helicobacter pylori and promotes cell growth and migration of gastric cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707351/
https://www.ncbi.nlm.nih.gov/pubmed/32021238
http://dx.doi.org/10.2147/OTT.S201832
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