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Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer
BACKGROUND: Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and Helicobacter pylori (H. pylori) is the major risk factor for GC. In this study, we focused on the roles of H. pylori-rela...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707351/ https://www.ncbi.nlm.nih.gov/pubmed/32021238 http://dx.doi.org/10.2147/OTT.S201832 |
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author | Jia, Wenxiao Zhang, Jiaqin Ma, Fang Hao, Shengjie Li, Xue Guo, Ruiting Gao, Qianqian Sun, Yundong Jia, Jihui Li, Wenjuan |
author_facet | Jia, Wenxiao Zhang, Jiaqin Ma, Fang Hao, Shengjie Li, Xue Guo, Ruiting Gao, Qianqian Sun, Yundong Jia, Jihui Li, Wenjuan |
author_sort | Jia, Wenxiao |
collection | PubMed |
description | BACKGROUND: Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and Helicobacter pylori (H. pylori) is the major risk factor for GC. In this study, we focused on the roles of H. pylori-related lncRNAs in the progression of GC. METHOD: Differentially expressed lncRNAs were identified through RNA-seq analysis of H. pylori-infected GC cells. RESULTS: We found that the expression of the lncRNA THAP9-AS1 was up-regulated after infection of GC cells with H. pylori and was higher in GC tissues than in gastritis tissues. Colony formation, CCK8 and transwell assays were executed to show that THAP9-AS1 can promote GC cell proliferation and migration in vitro. Our study identified the pro-oncogenic lncRNA THAP9-AS1, which has a higher expression level in GC tissues than in gastritis tissues and which promoted the proliferation and migration of GC cells in vitro. CONCLUSION: These findings may provide a potential therapeutic target for H. pylori-associated GC. |
format | Online Article Text |
id | pubmed-6707351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-67073512020-02-04 Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer Jia, Wenxiao Zhang, Jiaqin Ma, Fang Hao, Shengjie Li, Xue Guo, Ruiting Gao, Qianqian Sun, Yundong Jia, Jihui Li, Wenjuan Onco Targets Ther Original Research BACKGROUND: Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and Helicobacter pylori (H. pylori) is the major risk factor for GC. In this study, we focused on the roles of H. pylori-related lncRNAs in the progression of GC. METHOD: Differentially expressed lncRNAs were identified through RNA-seq analysis of H. pylori-infected GC cells. RESULTS: We found that the expression of the lncRNA THAP9-AS1 was up-regulated after infection of GC cells with H. pylori and was higher in GC tissues than in gastritis tissues. Colony formation, CCK8 and transwell assays were executed to show that THAP9-AS1 can promote GC cell proliferation and migration in vitro. Our study identified the pro-oncogenic lncRNA THAP9-AS1, which has a higher expression level in GC tissues than in gastritis tissues and which promoted the proliferation and migration of GC cells in vitro. CONCLUSION: These findings may provide a potential therapeutic target for H. pylori-associated GC. Dove 2019-08-19 /pmc/articles/PMC6707351/ /pubmed/32021238 http://dx.doi.org/10.2147/OTT.S201832 Text en © 2019 Jia et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Jia, Wenxiao Zhang, Jiaqin Ma, Fang Hao, Shengjie Li, Xue Guo, Ruiting Gao, Qianqian Sun, Yundong Jia, Jihui Li, Wenjuan Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer |
title | Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer |
title_full | Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer |
title_fullStr | Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer |
title_full_unstemmed | Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer |
title_short | Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer |
title_sort | long noncoding rna thap9-as1 is induced by helicobacter pylori and promotes cell growth and migration of gastric cancer |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707351/ https://www.ncbi.nlm.nih.gov/pubmed/32021238 http://dx.doi.org/10.2147/OTT.S201832 |
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