Cargando…

Overexpressed PKMYT1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma

BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant tumors worldwide and the 5-year overall survival rate remains poor. Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is overexpressed in several cancers and participate in tumor progression. Howe...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Qingyi, Zhao, Xuan, Zhang, Chaoqi, Wang, Wei, Li, Feng, Liu, Donglei, Wu, Kai, Zhu, Dengyan, Liu, Shasha, Shen, Chunyi, Yuan, Xin, Zhang, Kai, Yang, Yang, Zhang, Yi, Zhao, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707438/
https://www.ncbi.nlm.nih.gov/pubmed/31695486
http://dx.doi.org/10.2147/CMAR.S214243
_version_ 1783445861494161408
author Zhang, Qingyi
Zhao, Xuan
Zhang, Chaoqi
Wang, Wei
Li, Feng
Liu, Donglei
Wu, Kai
Zhu, Dengyan
Liu, Shasha
Shen, Chunyi
Yuan, Xin
Zhang, Kai
Yang, Yang
Zhang, Yi
Zhao, Song
author_facet Zhang, Qingyi
Zhao, Xuan
Zhang, Chaoqi
Wang, Wei
Li, Feng
Liu, Donglei
Wu, Kai
Zhu, Dengyan
Liu, Shasha
Shen, Chunyi
Yuan, Xin
Zhang, Kai
Yang, Yang
Zhang, Yi
Zhao, Song
author_sort Zhang, Qingyi
collection PubMed
description BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant tumors worldwide and the 5-year overall survival rate remains poor. Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is overexpressed in several cancers and participate in tumor progression. However, the mechanism of PKMYT1 in ESCC is unclear. PURPOSE: The objective of our study was to demonstrate the the expression and role of PKMYT1 in ESCC. PATIENTS AND METHODS:   We detected the expression of PKMYT1 in ESCC patients and analysed the correlation with overall survival time and disease-free survival time. Then we detected PKMYT1 expression in ESCC cell lines and immortalized human esophageal epithelial cell line. Down-regulated PKMYT1 was carried out in KYSE70 and KYSE450 cells to invetigate the mechanism of PKMYT1 in ESCC cells. RESULTS: PKMYT1 was up-regulated in tumor tissues and ESCC cell lines, and higher expression of PKMYT1 correlated with poorer overall survival in ESCC patients. Besides, in ESCC cell lines KYSE70 and KYSE450, knocking down PKMYT1 allowed more cells to skip G2/M checkpoint to complete mitosis, which promoted cell apoptosis, inhibited cell proliferation, and prevented the EMT phenotype in vitro. Meantime, we also observed that down-regulated PKMYT1 in ESCC cells suppressed AKT/mTOR signaling pathway. These results demonstrated PKMYT1 may act as an oncogene in ESCC. CONCLUSION: PKMYT1 plays an crutial role in ESCC progression, downregulated PKMYT1 might inhibit the development of ESCC by AKT/mTOR signaling pathway, and might be a novel target in the treatment of ESCC.
format Online
Article
Text
id pubmed-6707438
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-67074382019-11-06 Overexpressed PKMYT1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma Zhang, Qingyi Zhao, Xuan Zhang, Chaoqi Wang, Wei Li, Feng Liu, Donglei Wu, Kai Zhu, Dengyan Liu, Shasha Shen, Chunyi Yuan, Xin Zhang, Kai Yang, Yang Zhang, Yi Zhao, Song Cancer Manag Res Original Research BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant tumors worldwide and the 5-year overall survival rate remains poor. Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is overexpressed in several cancers and participate in tumor progression. However, the mechanism of PKMYT1 in ESCC is unclear. PURPOSE: The objective of our study was to demonstrate the the expression and role of PKMYT1 in ESCC. PATIENTS AND METHODS:   We detected the expression of PKMYT1 in ESCC patients and analysed the correlation with overall survival time and disease-free survival time. Then we detected PKMYT1 expression in ESCC cell lines and immortalized human esophageal epithelial cell line. Down-regulated PKMYT1 was carried out in KYSE70 and KYSE450 cells to invetigate the mechanism of PKMYT1 in ESCC cells. RESULTS: PKMYT1 was up-regulated in tumor tissues and ESCC cell lines, and higher expression of PKMYT1 correlated with poorer overall survival in ESCC patients. Besides, in ESCC cell lines KYSE70 and KYSE450, knocking down PKMYT1 allowed more cells to skip G2/M checkpoint to complete mitosis, which promoted cell apoptosis, inhibited cell proliferation, and prevented the EMT phenotype in vitro. Meantime, we also observed that down-regulated PKMYT1 in ESCC cells suppressed AKT/mTOR signaling pathway. These results demonstrated PKMYT1 may act as an oncogene in ESCC. CONCLUSION: PKMYT1 plays an crutial role in ESCC progression, downregulated PKMYT1 might inhibit the development of ESCC by AKT/mTOR signaling pathway, and might be a novel target in the treatment of ESCC. Dove 2019-08-19 /pmc/articles/PMC6707438/ /pubmed/31695486 http://dx.doi.org/10.2147/CMAR.S214243 Text en © 2019 Zhang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Zhang, Qingyi
Zhao, Xuan
Zhang, Chaoqi
Wang, Wei
Li, Feng
Liu, Donglei
Wu, Kai
Zhu, Dengyan
Liu, Shasha
Shen, Chunyi
Yuan, Xin
Zhang, Kai
Yang, Yang
Zhang, Yi
Zhao, Song
Overexpressed PKMYT1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma
title Overexpressed PKMYT1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma
title_full Overexpressed PKMYT1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma
title_fullStr Overexpressed PKMYT1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma
title_full_unstemmed Overexpressed PKMYT1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma
title_short Overexpressed PKMYT1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma
title_sort overexpressed pkmyt1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707438/
https://www.ncbi.nlm.nih.gov/pubmed/31695486
http://dx.doi.org/10.2147/CMAR.S214243
work_keys_str_mv AT zhangqingyi overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT zhaoxuan overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT zhangchaoqi overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT wangwei overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT lifeng overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT liudonglei overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT wukai overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT zhudengyan overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT liushasha overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT shenchunyi overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT yuanxin overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT zhangkai overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT yangyang overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT zhangyi overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma
AT zhaosong overexpressedpkmyt1promotestumorprogressionandassociateswithpoorsurvivalinesophagealsquamouscellcarcinoma