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Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli

Tigecycline is one of the last-resort antibiotics to treat complicated infections caused by both multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria1. Tigecycline resistance has sporadically occurred in recent years, primarily due to chromosome-encoding mechanisms, such as overexpress...

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Autores principales: Sun, Jian, Chen, Chong, Cui, Chao-Yue, Zhang, Yan, Liu, Xiao, Cui, Ze-Hua, Ma, Xiao-Yu, Feng, You-Jun, Fang, Liang-Xing, Lian, Xin-Lei, Zhang, Rong-Min, Tang, You-Zhi, Zhang, Kou-Xing, Liu, Han-Mian, Zhuang, Zhi-Hui, Zhou, Shi-Dan, Lv, Jing-Nan, Du, Hong, Huang, Bin, Yu, Fang-You, Mathema, Barun, Kreiswirth, Barry N., Liao, Xiao-Ping, Chen, Liang, Liu, Ya-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707864/
https://www.ncbi.nlm.nih.gov/pubmed/31235960
http://dx.doi.org/10.1038/s41564-019-0496-4
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author Sun, Jian
Chen, Chong
Cui, Chao-Yue
Zhang, Yan
Liu, Xiao
Cui, Ze-Hua
Ma, Xiao-Yu
Feng, You-Jun
Fang, Liang-Xing
Lian, Xin-Lei
Zhang, Rong-Min
Tang, You-Zhi
Zhang, Kou-Xing
Liu, Han-Mian
Zhuang, Zhi-Hui
Zhou, Shi-Dan
Lv, Jing-Nan
Du, Hong
Huang, Bin
Yu, Fang-You
Mathema, Barun
Kreiswirth, Barry N.
Liao, Xiao-Ping
Chen, Liang
Liu, Ya-Hong
author_facet Sun, Jian
Chen, Chong
Cui, Chao-Yue
Zhang, Yan
Liu, Xiao
Cui, Ze-Hua
Ma, Xiao-Yu
Feng, You-Jun
Fang, Liang-Xing
Lian, Xin-Lei
Zhang, Rong-Min
Tang, You-Zhi
Zhang, Kou-Xing
Liu, Han-Mian
Zhuang, Zhi-Hui
Zhou, Shi-Dan
Lv, Jing-Nan
Du, Hong
Huang, Bin
Yu, Fang-You
Mathema, Barun
Kreiswirth, Barry N.
Liao, Xiao-Ping
Chen, Liang
Liu, Ya-Hong
author_sort Sun, Jian
collection PubMed
description Tigecycline is one of the last-resort antibiotics to treat complicated infections caused by both multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria1. Tigecycline resistance has sporadically occurred in recent years, primarily due to chromosome-encoding mechanisms, such as overexpression of efflux pumps and ribosome protection(2,3). Here we report the emergence of plasmid-mediated mobile tigecycline resistance mechanism Tet(X4) in Escherichia coli isolates from China, which is capable of degrading all tetracyclines, including tigecycline and the FDA newly approved eravacycline. The tet(X4)-harboring IncQ1 plasmid is highly transferable, and can be successfully mobilized and stabilized in recipient clinical and laboratory strains of Enterobacteriaceae bacteria. It is noteworthy that tet(X4)-positive E. coli strains, including isolates co-harboring mcr-1, have been widely detected in pigs, chickens, soil, and dust samples in China. In vivo murine models demonstrated that the presence of Tet(X4) led to tigecycline treatment failure. Consequently, the emergence of plasmid-mediated Tet(X4) challenges the clinical efficacy of the entire family of tetracycline antibiotics. Importantly, our study raises concern that the plasmid-mediated tigecycline resistance may further spread into a variety of ecological niches and into clinical high-risk pathogens. Collective efforts are in urgent need to preserve the potency of these essential antibiotics.
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spelling pubmed-67078642019-12-24 Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli Sun, Jian Chen, Chong Cui, Chao-Yue Zhang, Yan Liu, Xiao Cui, Ze-Hua Ma, Xiao-Yu Feng, You-Jun Fang, Liang-Xing Lian, Xin-Lei Zhang, Rong-Min Tang, You-Zhi Zhang, Kou-Xing Liu, Han-Mian Zhuang, Zhi-Hui Zhou, Shi-Dan Lv, Jing-Nan Du, Hong Huang, Bin Yu, Fang-You Mathema, Barun Kreiswirth, Barry N. Liao, Xiao-Ping Chen, Liang Liu, Ya-Hong Nat Microbiol Article Tigecycline is one of the last-resort antibiotics to treat complicated infections caused by both multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria1. Tigecycline resistance has sporadically occurred in recent years, primarily due to chromosome-encoding mechanisms, such as overexpression of efflux pumps and ribosome protection(2,3). Here we report the emergence of plasmid-mediated mobile tigecycline resistance mechanism Tet(X4) in Escherichia coli isolates from China, which is capable of degrading all tetracyclines, including tigecycline and the FDA newly approved eravacycline. The tet(X4)-harboring IncQ1 plasmid is highly transferable, and can be successfully mobilized and stabilized in recipient clinical and laboratory strains of Enterobacteriaceae bacteria. It is noteworthy that tet(X4)-positive E. coli strains, including isolates co-harboring mcr-1, have been widely detected in pigs, chickens, soil, and dust samples in China. In vivo murine models demonstrated that the presence of Tet(X4) led to tigecycline treatment failure. Consequently, the emergence of plasmid-mediated Tet(X4) challenges the clinical efficacy of the entire family of tetracycline antibiotics. Importantly, our study raises concern that the plasmid-mediated tigecycline resistance may further spread into a variety of ecological niches and into clinical high-risk pathogens. Collective efforts are in urgent need to preserve the potency of these essential antibiotics. 2019-06-24 2019-09 /pmc/articles/PMC6707864/ /pubmed/31235960 http://dx.doi.org/10.1038/s41564-019-0496-4 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Sun, Jian
Chen, Chong
Cui, Chao-Yue
Zhang, Yan
Liu, Xiao
Cui, Ze-Hua
Ma, Xiao-Yu
Feng, You-Jun
Fang, Liang-Xing
Lian, Xin-Lei
Zhang, Rong-Min
Tang, You-Zhi
Zhang, Kou-Xing
Liu, Han-Mian
Zhuang, Zhi-Hui
Zhou, Shi-Dan
Lv, Jing-Nan
Du, Hong
Huang, Bin
Yu, Fang-You
Mathema, Barun
Kreiswirth, Barry N.
Liao, Xiao-Ping
Chen, Liang
Liu, Ya-Hong
Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli
title Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli
title_full Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli
title_fullStr Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli
title_full_unstemmed Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli
title_short Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli
title_sort plasmid-encoded tet(x) genes that confer high-level tigecycline resistance in escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707864/
https://www.ncbi.nlm.nih.gov/pubmed/31235960
http://dx.doi.org/10.1038/s41564-019-0496-4
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