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Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli
Tigecycline is one of the last-resort antibiotics to treat complicated infections caused by both multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria1. Tigecycline resistance has sporadically occurred in recent years, primarily due to chromosome-encoding mechanisms, such as overexpress...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707864/ https://www.ncbi.nlm.nih.gov/pubmed/31235960 http://dx.doi.org/10.1038/s41564-019-0496-4 |
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author | Sun, Jian Chen, Chong Cui, Chao-Yue Zhang, Yan Liu, Xiao Cui, Ze-Hua Ma, Xiao-Yu Feng, You-Jun Fang, Liang-Xing Lian, Xin-Lei Zhang, Rong-Min Tang, You-Zhi Zhang, Kou-Xing Liu, Han-Mian Zhuang, Zhi-Hui Zhou, Shi-Dan Lv, Jing-Nan Du, Hong Huang, Bin Yu, Fang-You Mathema, Barun Kreiswirth, Barry N. Liao, Xiao-Ping Chen, Liang Liu, Ya-Hong |
author_facet | Sun, Jian Chen, Chong Cui, Chao-Yue Zhang, Yan Liu, Xiao Cui, Ze-Hua Ma, Xiao-Yu Feng, You-Jun Fang, Liang-Xing Lian, Xin-Lei Zhang, Rong-Min Tang, You-Zhi Zhang, Kou-Xing Liu, Han-Mian Zhuang, Zhi-Hui Zhou, Shi-Dan Lv, Jing-Nan Du, Hong Huang, Bin Yu, Fang-You Mathema, Barun Kreiswirth, Barry N. Liao, Xiao-Ping Chen, Liang Liu, Ya-Hong |
author_sort | Sun, Jian |
collection | PubMed |
description | Tigecycline is one of the last-resort antibiotics to treat complicated infections caused by both multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria1. Tigecycline resistance has sporadically occurred in recent years, primarily due to chromosome-encoding mechanisms, such as overexpression of efflux pumps and ribosome protection(2,3). Here we report the emergence of plasmid-mediated mobile tigecycline resistance mechanism Tet(X4) in Escherichia coli isolates from China, which is capable of degrading all tetracyclines, including tigecycline and the FDA newly approved eravacycline. The tet(X4)-harboring IncQ1 plasmid is highly transferable, and can be successfully mobilized and stabilized in recipient clinical and laboratory strains of Enterobacteriaceae bacteria. It is noteworthy that tet(X4)-positive E. coli strains, including isolates co-harboring mcr-1, have been widely detected in pigs, chickens, soil, and dust samples in China. In vivo murine models demonstrated that the presence of Tet(X4) led to tigecycline treatment failure. Consequently, the emergence of plasmid-mediated Tet(X4) challenges the clinical efficacy of the entire family of tetracycline antibiotics. Importantly, our study raises concern that the plasmid-mediated tigecycline resistance may further spread into a variety of ecological niches and into clinical high-risk pathogens. Collective efforts are in urgent need to preserve the potency of these essential antibiotics. |
format | Online Article Text |
id | pubmed-6707864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-67078642019-12-24 Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli Sun, Jian Chen, Chong Cui, Chao-Yue Zhang, Yan Liu, Xiao Cui, Ze-Hua Ma, Xiao-Yu Feng, You-Jun Fang, Liang-Xing Lian, Xin-Lei Zhang, Rong-Min Tang, You-Zhi Zhang, Kou-Xing Liu, Han-Mian Zhuang, Zhi-Hui Zhou, Shi-Dan Lv, Jing-Nan Du, Hong Huang, Bin Yu, Fang-You Mathema, Barun Kreiswirth, Barry N. Liao, Xiao-Ping Chen, Liang Liu, Ya-Hong Nat Microbiol Article Tigecycline is one of the last-resort antibiotics to treat complicated infections caused by both multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria1. Tigecycline resistance has sporadically occurred in recent years, primarily due to chromosome-encoding mechanisms, such as overexpression of efflux pumps and ribosome protection(2,3). Here we report the emergence of plasmid-mediated mobile tigecycline resistance mechanism Tet(X4) in Escherichia coli isolates from China, which is capable of degrading all tetracyclines, including tigecycline and the FDA newly approved eravacycline. The tet(X4)-harboring IncQ1 plasmid is highly transferable, and can be successfully mobilized and stabilized in recipient clinical and laboratory strains of Enterobacteriaceae bacteria. It is noteworthy that tet(X4)-positive E. coli strains, including isolates co-harboring mcr-1, have been widely detected in pigs, chickens, soil, and dust samples in China. In vivo murine models demonstrated that the presence of Tet(X4) led to tigecycline treatment failure. Consequently, the emergence of plasmid-mediated Tet(X4) challenges the clinical efficacy of the entire family of tetracycline antibiotics. Importantly, our study raises concern that the plasmid-mediated tigecycline resistance may further spread into a variety of ecological niches and into clinical high-risk pathogens. Collective efforts are in urgent need to preserve the potency of these essential antibiotics. 2019-06-24 2019-09 /pmc/articles/PMC6707864/ /pubmed/31235960 http://dx.doi.org/10.1038/s41564-019-0496-4 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Sun, Jian Chen, Chong Cui, Chao-Yue Zhang, Yan Liu, Xiao Cui, Ze-Hua Ma, Xiao-Yu Feng, You-Jun Fang, Liang-Xing Lian, Xin-Lei Zhang, Rong-Min Tang, You-Zhi Zhang, Kou-Xing Liu, Han-Mian Zhuang, Zhi-Hui Zhou, Shi-Dan Lv, Jing-Nan Du, Hong Huang, Bin Yu, Fang-You Mathema, Barun Kreiswirth, Barry N. Liao, Xiao-Ping Chen, Liang Liu, Ya-Hong Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli |
title | Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli |
title_full | Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli |
title_fullStr | Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli |
title_full_unstemmed | Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli |
title_short | Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli |
title_sort | plasmid-encoded tet(x) genes that confer high-level tigecycline resistance in escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707864/ https://www.ncbi.nlm.nih.gov/pubmed/31235960 http://dx.doi.org/10.1038/s41564-019-0496-4 |
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