Cargando…
Comparative iTRAQ-based quantitative proteomic analysis of the Chinese grass shrimp (Palaemonetes sinensis) infected with the isopod parasite Tachaea chinensis
BACKGROUND: Although parasitic isopods can negatively affect the reproduction and ingestion of several commercially important crustaceans, little is known regarding the mechanisms that underlie these effects. METHODS: In the present study, the iTRAQ (isobaric tags for relative and absolute quantific...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6708196/ https://www.ncbi.nlm.nih.gov/pubmed/31443734 http://dx.doi.org/10.1186/s13071-019-3675-5 |
_version_ | 1783445972599177216 |
---|---|
author | Li, Yingdong Li, Xin Xu, Weibin Han, Zhibin Zhao, Yingying Dong, Jing Wei, Hua Chen, Qijun |
author_facet | Li, Yingdong Li, Xin Xu, Weibin Han, Zhibin Zhao, Yingying Dong, Jing Wei, Hua Chen, Qijun |
author_sort | Li, Yingdong |
collection | PubMed |
description | BACKGROUND: Although parasitic isopods can negatively affect the reproduction and ingestion of several commercially important crustaceans, little is known regarding the mechanisms that underlie these effects. METHODS: In the present study, the iTRAQ (isobaric tags for relative and absolute quantification) approach was applied to identify differentially expressed proteins in the Chinese grass shrimp Palaemonetes sinensis infected with the parasitic isopod Tachaea chinensis. RESULTS: On the basis of our analysis, we identified 1262 proteins from a total of 4292 peptides. There was a significant difference in the expression of 182 proteins between the control and infected groups, among which 69 were upregulated and 113 were downregulated after T. chinensis infection. The differentially expressed proteins revealed that parasitism may inhibit the immune response, thereby increasing host vulnerability to additional lethal infection. Furthermore, T. chinensis may secrete anticoagulants to inhibit hemolymph clotting. Moreover, the isopod parasite placed a heavy metabolic burden on the host, particularly with respect to glucose metabolism. CONCLUSIONS: Our study is the first to use the iTRAQ-based proteomic approach to analyze the effects of an isopod parasite on its host. The results we obtained using this approach will make a valuable contribution to understanding the molecular mechanisms underlying isopod parasitism on crustaceans. |
format | Online Article Text |
id | pubmed-6708196 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67081962019-08-28 Comparative iTRAQ-based quantitative proteomic analysis of the Chinese grass shrimp (Palaemonetes sinensis) infected with the isopod parasite Tachaea chinensis Li, Yingdong Li, Xin Xu, Weibin Han, Zhibin Zhao, Yingying Dong, Jing Wei, Hua Chen, Qijun Parasit Vectors Research BACKGROUND: Although parasitic isopods can negatively affect the reproduction and ingestion of several commercially important crustaceans, little is known regarding the mechanisms that underlie these effects. METHODS: In the present study, the iTRAQ (isobaric tags for relative and absolute quantification) approach was applied to identify differentially expressed proteins in the Chinese grass shrimp Palaemonetes sinensis infected with the parasitic isopod Tachaea chinensis. RESULTS: On the basis of our analysis, we identified 1262 proteins from a total of 4292 peptides. There was a significant difference in the expression of 182 proteins between the control and infected groups, among which 69 were upregulated and 113 were downregulated after T. chinensis infection. The differentially expressed proteins revealed that parasitism may inhibit the immune response, thereby increasing host vulnerability to additional lethal infection. Furthermore, T. chinensis may secrete anticoagulants to inhibit hemolymph clotting. Moreover, the isopod parasite placed a heavy metabolic burden on the host, particularly with respect to glucose metabolism. CONCLUSIONS: Our study is the first to use the iTRAQ-based proteomic approach to analyze the effects of an isopod parasite on its host. The results we obtained using this approach will make a valuable contribution to understanding the molecular mechanisms underlying isopod parasitism on crustaceans. BioMed Central 2019-08-23 /pmc/articles/PMC6708196/ /pubmed/31443734 http://dx.doi.org/10.1186/s13071-019-3675-5 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Li, Yingdong Li, Xin Xu, Weibin Han, Zhibin Zhao, Yingying Dong, Jing Wei, Hua Chen, Qijun Comparative iTRAQ-based quantitative proteomic analysis of the Chinese grass shrimp (Palaemonetes sinensis) infected with the isopod parasite Tachaea chinensis |
title | Comparative iTRAQ-based quantitative proteomic analysis of the Chinese grass shrimp (Palaemonetes sinensis) infected with the isopod parasite Tachaea chinensis |
title_full | Comparative iTRAQ-based quantitative proteomic analysis of the Chinese grass shrimp (Palaemonetes sinensis) infected with the isopod parasite Tachaea chinensis |
title_fullStr | Comparative iTRAQ-based quantitative proteomic analysis of the Chinese grass shrimp (Palaemonetes sinensis) infected with the isopod parasite Tachaea chinensis |
title_full_unstemmed | Comparative iTRAQ-based quantitative proteomic analysis of the Chinese grass shrimp (Palaemonetes sinensis) infected with the isopod parasite Tachaea chinensis |
title_short | Comparative iTRAQ-based quantitative proteomic analysis of the Chinese grass shrimp (Palaemonetes sinensis) infected with the isopod parasite Tachaea chinensis |
title_sort | comparative itraq-based quantitative proteomic analysis of the chinese grass shrimp (palaemonetes sinensis) infected with the isopod parasite tachaea chinensis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6708196/ https://www.ncbi.nlm.nih.gov/pubmed/31443734 http://dx.doi.org/10.1186/s13071-019-3675-5 |
work_keys_str_mv | AT liyingdong comparativeitraqbasedquantitativeproteomicanalysisofthechinesegrassshrimppalaemonetessinensisinfectedwiththeisopodparasitetachaeachinensis AT lixin comparativeitraqbasedquantitativeproteomicanalysisofthechinesegrassshrimppalaemonetessinensisinfectedwiththeisopodparasitetachaeachinensis AT xuweibin comparativeitraqbasedquantitativeproteomicanalysisofthechinesegrassshrimppalaemonetessinensisinfectedwiththeisopodparasitetachaeachinensis AT hanzhibin comparativeitraqbasedquantitativeproteomicanalysisofthechinesegrassshrimppalaemonetessinensisinfectedwiththeisopodparasitetachaeachinensis AT zhaoyingying comparativeitraqbasedquantitativeproteomicanalysisofthechinesegrassshrimppalaemonetessinensisinfectedwiththeisopodparasitetachaeachinensis AT dongjing comparativeitraqbasedquantitativeproteomicanalysisofthechinesegrassshrimppalaemonetessinensisinfectedwiththeisopodparasitetachaeachinensis AT weihua comparativeitraqbasedquantitativeproteomicanalysisofthechinesegrassshrimppalaemonetessinensisinfectedwiththeisopodparasitetachaeachinensis AT chenqijun comparativeitraqbasedquantitativeproteomicanalysisofthechinesegrassshrimppalaemonetessinensisinfectedwiththeisopodparasitetachaeachinensis |