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Detection and genetic characterization of Giardia duodenalis in pigs from large-scale farms in Xinjiang, China

To study the presence of Giardia duodenalis in Xinjiang, northwest China, we collected 801 fecal specimens from seven large-scale pig farms and screened them using PCR targeting the SSU rRNA gene. Twenty-one (2.6%) of the specimens from five farms were G. duodenalis-positive, with a significant diff...

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Autores principales: Jing, Bo, Zhang, Ying, Xu, Chunyan, Li, Dongfang, Xing, Jinming, Tao, Dayong, Zhang, Longxian, Qi, Meng, Wang, Haiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6709646/
https://www.ncbi.nlm.nih.gov/pubmed/31448999
http://dx.doi.org/10.1051/parasite/2019056
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author Jing, Bo
Zhang, Ying
Xu, Chunyan
Li, Dongfang
Xing, Jinming
Tao, Dayong
Zhang, Longxian
Qi, Meng
Wang, Haiyan
author_facet Jing, Bo
Zhang, Ying
Xu, Chunyan
Li, Dongfang
Xing, Jinming
Tao, Dayong
Zhang, Longxian
Qi, Meng
Wang, Haiyan
author_sort Jing, Bo
collection PubMed
description To study the presence of Giardia duodenalis in Xinjiang, northwest China, we collected 801 fecal specimens from seven large-scale pig farms and screened them using PCR targeting the SSU rRNA gene. Twenty-one (2.6%) of the specimens from five farms were G. duodenalis-positive, with a significant difference in prevalence among different farms (0–8.7%) (p < 0.01). Giardia duodenalis prevalence was highest in fattening pigs (5.4%, 7/129), followed by sows (3.2%, 7/222), post-weaning piglets (1.8%, 5/281), and pre-weaning piglets (1.2%, 2/169), but there was no significant difference in prevalence among the age groups (p > 0.05). Sequence analysis of the SSU rRNA gene revealed that the 21 G. duodenalis strains belonged to three assemblages: A (n = 2), B (n = 16), and E (n = 3). Assemblage B was the predominant assemblage and was widely distributed in all G. duodenalis-positive farms and age groups. All G. duodenalis-positive specimens were further assayed at the β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes, and two tpi, four gdh, and two bg sequences were identified. These data indicate that pigs may be a zoonotic risk and can potentially spread G. duodenalis infection from animals to humans.
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spelling pubmed-67096462019-09-12 Detection and genetic characterization of Giardia duodenalis in pigs from large-scale farms in Xinjiang, China Jing, Bo Zhang, Ying Xu, Chunyan Li, Dongfang Xing, Jinming Tao, Dayong Zhang, Longxian Qi, Meng Wang, Haiyan Parasite Research Article To study the presence of Giardia duodenalis in Xinjiang, northwest China, we collected 801 fecal specimens from seven large-scale pig farms and screened them using PCR targeting the SSU rRNA gene. Twenty-one (2.6%) of the specimens from five farms were G. duodenalis-positive, with a significant difference in prevalence among different farms (0–8.7%) (p < 0.01). Giardia duodenalis prevalence was highest in fattening pigs (5.4%, 7/129), followed by sows (3.2%, 7/222), post-weaning piglets (1.8%, 5/281), and pre-weaning piglets (1.2%, 2/169), but there was no significant difference in prevalence among the age groups (p > 0.05). Sequence analysis of the SSU rRNA gene revealed that the 21 G. duodenalis strains belonged to three assemblages: A (n = 2), B (n = 16), and E (n = 3). Assemblage B was the predominant assemblage and was widely distributed in all G. duodenalis-positive farms and age groups. All G. duodenalis-positive specimens were further assayed at the β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes, and two tpi, four gdh, and two bg sequences were identified. These data indicate that pigs may be a zoonotic risk and can potentially spread G. duodenalis infection from animals to humans. EDP Sciences 2019-08-26 /pmc/articles/PMC6709646/ /pubmed/31448999 http://dx.doi.org/10.1051/parasite/2019056 Text en © B. Jing et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jing, Bo
Zhang, Ying
Xu, Chunyan
Li, Dongfang
Xing, Jinming
Tao, Dayong
Zhang, Longxian
Qi, Meng
Wang, Haiyan
Detection and genetic characterization of Giardia duodenalis in pigs from large-scale farms in Xinjiang, China
title Detection and genetic characterization of Giardia duodenalis in pigs from large-scale farms in Xinjiang, China
title_full Detection and genetic characterization of Giardia duodenalis in pigs from large-scale farms in Xinjiang, China
title_fullStr Detection and genetic characterization of Giardia duodenalis in pigs from large-scale farms in Xinjiang, China
title_full_unstemmed Detection and genetic characterization of Giardia duodenalis in pigs from large-scale farms in Xinjiang, China
title_short Detection and genetic characterization of Giardia duodenalis in pigs from large-scale farms in Xinjiang, China
title_sort detection and genetic characterization of giardia duodenalis in pigs from large-scale farms in xinjiang, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6709646/
https://www.ncbi.nlm.nih.gov/pubmed/31448999
http://dx.doi.org/10.1051/parasite/2019056
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