LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism

PURPOSE: The present study aims to investigate the role of ELF3-AS1 in oral squamous cell carcinoma (OSCC). PATIENTS AND METHODS: A total of 112 patients with OSCC were admitted in Guangdong Provincial Stomatological Hospital from March 2016 to March 2019. RT-qPCR, cells and transient transfections,...

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Detalles Bibliográficos
Autores principales: Chu, Hongxing, Li, Zhengqiang, Gan, Zekun, Yang, Zinan, Wu, Zhenzhen, Rong, Mingdeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6709795/
https://www.ncbi.nlm.nih.gov/pubmed/31686842
http://dx.doi.org/10.2147/OTT.S217473
Descripción
Sumario:PURPOSE: The present study aims to investigate the role of ELF3-AS1 in oral squamous cell carcinoma (OSCC). PATIENTS AND METHODS: A total of 112 patients with OSCC were admitted in Guangdong Provincial Stomatological Hospital from March 2016 to March 2019. RT-qPCR, cells and transient transfections, cell proliferation rate measurements and Western blots were carried out to analyze the samples. RESULTS: In the present study, we showed that ELF3-AS1 and glucose transporter 1 (GLUT1) were both upregulated in OSCC tissues, and those two factors were positively correlated. In OSCC cells, ELF3-AS1 overexpression resulted in upregulation, while ELF3-AS1 siRNA silencing caused downregulated expression of GLUT1 and glucose uptake. ELF3-AS1 and GLUT1 overexpression resulted in increased rate of OSCC cells, while ELF3-AS1 and GLUT1 siRNA silencing resulted in decreased proliferation rate of OSCC cells. In addition, GLUT1 siRNA silencing attenuated the effects of ELF3-AS1 overexpression. CONCLUSION: Therefore, ELF3-AS1 promotes the proliferation of OSCC cells by reprogramming glucose metabolism.

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