LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism

PURPOSE: The present study aims to investigate the role of ELF3-AS1 in oral squamous cell carcinoma (OSCC). PATIENTS AND METHODS: A total of 112 patients with OSCC were admitted in Guangdong Provincial Stomatological Hospital from March 2016 to March 2019. RT-qPCR, cells and transient transfections,...

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Autores principales: Chu, Hongxing, Li, Zhengqiang, Gan, Zekun, Yang, Zinan, Wu, Zhenzhen, Rong, Mingdeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6709795/
https://www.ncbi.nlm.nih.gov/pubmed/31686842
http://dx.doi.org/10.2147/OTT.S217473
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author Chu, Hongxing
Li, Zhengqiang
Gan, Zekun
Yang, Zinan
Wu, Zhenzhen
Rong, Mingdeng
author_facet Chu, Hongxing
Li, Zhengqiang
Gan, Zekun
Yang, Zinan
Wu, Zhenzhen
Rong, Mingdeng
author_sort Chu, Hongxing
collection PubMed
description PURPOSE: The present study aims to investigate the role of ELF3-AS1 in oral squamous cell carcinoma (OSCC). PATIENTS AND METHODS: A total of 112 patients with OSCC were admitted in Guangdong Provincial Stomatological Hospital from March 2016 to March 2019. RT-qPCR, cells and transient transfections, cell proliferation rate measurements and Western blots were carried out to analyze the samples. RESULTS: In the present study, we showed that ELF3-AS1 and glucose transporter 1 (GLUT1) were both upregulated in OSCC tissues, and those two factors were positively correlated. In OSCC cells, ELF3-AS1 overexpression resulted in upregulation, while ELF3-AS1 siRNA silencing caused downregulated expression of GLUT1 and glucose uptake. ELF3-AS1 and GLUT1 overexpression resulted in increased rate of OSCC cells, while ELF3-AS1 and GLUT1 siRNA silencing resulted in decreased proliferation rate of OSCC cells. In addition, GLUT1 siRNA silencing attenuated the effects of ELF3-AS1 overexpression. CONCLUSION: Therefore, ELF3-AS1 promotes the proliferation of OSCC cells by reprogramming glucose metabolism.
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spelling pubmed-67097952019-11-04 LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism Chu, Hongxing Li, Zhengqiang Gan, Zekun Yang, Zinan Wu, Zhenzhen Rong, Mingdeng Onco Targets Ther Original Research PURPOSE: The present study aims to investigate the role of ELF3-AS1 in oral squamous cell carcinoma (OSCC). PATIENTS AND METHODS: A total of 112 patients with OSCC were admitted in Guangdong Provincial Stomatological Hospital from March 2016 to March 2019. RT-qPCR, cells and transient transfections, cell proliferation rate measurements and Western blots were carried out to analyze the samples. RESULTS: In the present study, we showed that ELF3-AS1 and glucose transporter 1 (GLUT1) were both upregulated in OSCC tissues, and those two factors were positively correlated. In OSCC cells, ELF3-AS1 overexpression resulted in upregulation, while ELF3-AS1 siRNA silencing caused downregulated expression of GLUT1 and glucose uptake. ELF3-AS1 and GLUT1 overexpression resulted in increased rate of OSCC cells, while ELF3-AS1 and GLUT1 siRNA silencing resulted in decreased proliferation rate of OSCC cells. In addition, GLUT1 siRNA silencing attenuated the effects of ELF3-AS1 overexpression. CONCLUSION: Therefore, ELF3-AS1 promotes the proliferation of OSCC cells by reprogramming glucose metabolism. Dove 2019-08-22 /pmc/articles/PMC6709795/ /pubmed/31686842 http://dx.doi.org/10.2147/OTT.S217473 Text en © 2019 Chu et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Chu, Hongxing
Li, Zhengqiang
Gan, Zekun
Yang, Zinan
Wu, Zhenzhen
Rong, Mingdeng
LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism
title LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism
title_full LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism
title_fullStr LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism
title_full_unstemmed LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism
title_short LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism
title_sort lncrna elf3-as1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6709795/
https://www.ncbi.nlm.nih.gov/pubmed/31686842
http://dx.doi.org/10.2147/OTT.S217473
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