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Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer
Evidence indicates that aberrantly expressed long non-coding RNAs (lncRNAs) are involved in the development and progression of advanced gastric cancer (AGC). Using RNA sequencing data and clinical information obtained from The Cancer Gene Atlas, we combined differential lncRNA expression profiling a...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710062/ https://www.ncbi.nlm.nih.gov/pubmed/31422382 http://dx.doi.org/10.18632/aging.102190 |
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author | Wang, Wen-Jie Guo, Chang-An Li, Rui Xu, Zi-Peng Yu, Jian-Ping Ye, Yan Zhao, Jun Wang, Jing Wang, Wen-An Zhang, An Li, Hong-Tao Wang, Chen Liu, Hong-Bin |
author_facet | Wang, Wen-Jie Guo, Chang-An Li, Rui Xu, Zi-Peng Yu, Jian-Ping Ye, Yan Zhao, Jun Wang, Jing Wang, Wen-An Zhang, An Li, Hong-Tao Wang, Chen Liu, Hong-Bin |
author_sort | Wang, Wen-Jie |
collection | PubMed |
description | Evidence indicates that aberrantly expressed long non-coding RNAs (lncRNAs) are involved in the development and progression of advanced gastric cancer (AGC). Using RNA sequencing data and clinical information obtained from The Cancer Gene Atlas, we combined differential lncRNA expression profiling and weighted gene co-expression network analysis to identify key lncRNAs associated with AGC progression and prognosis. Cancer susceptibility 19 (CASC19) was the top hub lncRNA among the lncRNAs included in the gene module most significantly correlated with AGC’s pathological variables. CASC19 was upregulated in AGC clinical samples and was significantly associated with higher pathologic TNM stage, pathologic T stage, lymph node metastasis, and poor overall survival. Multivariable Cox analysis confirmed that CASC19 overexpression is an independent prognostic factor for overall survival. Furthermore, quantitative real-time PCR assay confirmed that CASC19 expression in four human gastric cancer cells (AGS, BGC-823, MGC-803, and HGC-27) was significantly upregulated compared with human normal gastric mucosal epithelial cell line (GES-1). Functionally, CASC19 knockdown inhibited GC cell proliferation and migration in vitro. These findings suggest that CASC19 may be a novel prognostic biomarker and a potential therapeutic target for AGC. |
format | Online Article Text |
id | pubmed-6710062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-67100622019-09-05 Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer Wang, Wen-Jie Guo, Chang-An Li, Rui Xu, Zi-Peng Yu, Jian-Ping Ye, Yan Zhao, Jun Wang, Jing Wang, Wen-An Zhang, An Li, Hong-Tao Wang, Chen Liu, Hong-Bin Aging (Albany NY) Research Paper Evidence indicates that aberrantly expressed long non-coding RNAs (lncRNAs) are involved in the development and progression of advanced gastric cancer (AGC). Using RNA sequencing data and clinical information obtained from The Cancer Gene Atlas, we combined differential lncRNA expression profiling and weighted gene co-expression network analysis to identify key lncRNAs associated with AGC progression and prognosis. Cancer susceptibility 19 (CASC19) was the top hub lncRNA among the lncRNAs included in the gene module most significantly correlated with AGC’s pathological variables. CASC19 was upregulated in AGC clinical samples and was significantly associated with higher pathologic TNM stage, pathologic T stage, lymph node metastasis, and poor overall survival. Multivariable Cox analysis confirmed that CASC19 overexpression is an independent prognostic factor for overall survival. Furthermore, quantitative real-time PCR assay confirmed that CASC19 expression in four human gastric cancer cells (AGS, BGC-823, MGC-803, and HGC-27) was significantly upregulated compared with human normal gastric mucosal epithelial cell line (GES-1). Functionally, CASC19 knockdown inhibited GC cell proliferation and migration in vitro. These findings suggest that CASC19 may be a novel prognostic biomarker and a potential therapeutic target for AGC. Impact Journals 2019-08-15 /pmc/articles/PMC6710062/ /pubmed/31422382 http://dx.doi.org/10.18632/aging.102190 Text en Copyright © 2019 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wang, Wen-Jie Guo, Chang-An Li, Rui Xu, Zi-Peng Yu, Jian-Ping Ye, Yan Zhao, Jun Wang, Jing Wang, Wen-An Zhang, An Li, Hong-Tao Wang, Chen Liu, Hong-Bin Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer |
title | Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer |
title_full | Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer |
title_fullStr | Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer |
title_full_unstemmed | Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer |
title_short | Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer |
title_sort | long non-coding rna casc19 is associated with the progression and prognosis of advanced gastric cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710062/ https://www.ncbi.nlm.nih.gov/pubmed/31422382 http://dx.doi.org/10.18632/aging.102190 |
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