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Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer

Evidence indicates that aberrantly expressed long non-coding RNAs (lncRNAs) are involved in the development and progression of advanced gastric cancer (AGC). Using RNA sequencing data and clinical information obtained from The Cancer Gene Atlas, we combined differential lncRNA expression profiling a...

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Autores principales: Wang, Wen-Jie, Guo, Chang-An, Li, Rui, Xu, Zi-Peng, Yu, Jian-Ping, Ye, Yan, Zhao, Jun, Wang, Jing, Wang, Wen-An, Zhang, An, Li, Hong-Tao, Wang, Chen, Liu, Hong-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710062/
https://www.ncbi.nlm.nih.gov/pubmed/31422382
http://dx.doi.org/10.18632/aging.102190
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author Wang, Wen-Jie
Guo, Chang-An
Li, Rui
Xu, Zi-Peng
Yu, Jian-Ping
Ye, Yan
Zhao, Jun
Wang, Jing
Wang, Wen-An
Zhang, An
Li, Hong-Tao
Wang, Chen
Liu, Hong-Bin
author_facet Wang, Wen-Jie
Guo, Chang-An
Li, Rui
Xu, Zi-Peng
Yu, Jian-Ping
Ye, Yan
Zhao, Jun
Wang, Jing
Wang, Wen-An
Zhang, An
Li, Hong-Tao
Wang, Chen
Liu, Hong-Bin
author_sort Wang, Wen-Jie
collection PubMed
description Evidence indicates that aberrantly expressed long non-coding RNAs (lncRNAs) are involved in the development and progression of advanced gastric cancer (AGC). Using RNA sequencing data and clinical information obtained from The Cancer Gene Atlas, we combined differential lncRNA expression profiling and weighted gene co-expression network analysis to identify key lncRNAs associated with AGC progression and prognosis. Cancer susceptibility 19 (CASC19) was the top hub lncRNA among the lncRNAs included in the gene module most significantly correlated with AGC’s pathological variables. CASC19 was upregulated in AGC clinical samples and was significantly associated with higher pathologic TNM stage, pathologic T stage, lymph node metastasis, and poor overall survival. Multivariable Cox analysis confirmed that CASC19 overexpression is an independent prognostic factor for overall survival. Furthermore, quantitative real-time PCR assay confirmed that CASC19 expression in four human gastric cancer cells (AGS, BGC-823, MGC-803, and HGC-27) was significantly upregulated compared with human normal gastric mucosal epithelial cell line (GES-1). Functionally, CASC19 knockdown inhibited GC cell proliferation and migration in vitro. These findings suggest that CASC19 may be a novel prognostic biomarker and a potential therapeutic target for AGC.
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spelling pubmed-67100622019-09-05 Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer Wang, Wen-Jie Guo, Chang-An Li, Rui Xu, Zi-Peng Yu, Jian-Ping Ye, Yan Zhao, Jun Wang, Jing Wang, Wen-An Zhang, An Li, Hong-Tao Wang, Chen Liu, Hong-Bin Aging (Albany NY) Research Paper Evidence indicates that aberrantly expressed long non-coding RNAs (lncRNAs) are involved in the development and progression of advanced gastric cancer (AGC). Using RNA sequencing data and clinical information obtained from The Cancer Gene Atlas, we combined differential lncRNA expression profiling and weighted gene co-expression network analysis to identify key lncRNAs associated with AGC progression and prognosis. Cancer susceptibility 19 (CASC19) was the top hub lncRNA among the lncRNAs included in the gene module most significantly correlated with AGC’s pathological variables. CASC19 was upregulated in AGC clinical samples and was significantly associated with higher pathologic TNM stage, pathologic T stage, lymph node metastasis, and poor overall survival. Multivariable Cox analysis confirmed that CASC19 overexpression is an independent prognostic factor for overall survival. Furthermore, quantitative real-time PCR assay confirmed that CASC19 expression in four human gastric cancer cells (AGS, BGC-823, MGC-803, and HGC-27) was significantly upregulated compared with human normal gastric mucosal epithelial cell line (GES-1). Functionally, CASC19 knockdown inhibited GC cell proliferation and migration in vitro. These findings suggest that CASC19 may be a novel prognostic biomarker and a potential therapeutic target for AGC. Impact Journals 2019-08-15 /pmc/articles/PMC6710062/ /pubmed/31422382 http://dx.doi.org/10.18632/aging.102190 Text en Copyright © 2019 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wang, Wen-Jie
Guo, Chang-An
Li, Rui
Xu, Zi-Peng
Yu, Jian-Ping
Ye, Yan
Zhao, Jun
Wang, Jing
Wang, Wen-An
Zhang, An
Li, Hong-Tao
Wang, Chen
Liu, Hong-Bin
Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer
title Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer
title_full Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer
title_fullStr Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer
title_full_unstemmed Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer
title_short Long non-coding RNA CASC19 is associated with the progression and prognosis of advanced gastric cancer
title_sort long non-coding rna casc19 is associated with the progression and prognosis of advanced gastric cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710062/
https://www.ncbi.nlm.nih.gov/pubmed/31422382
http://dx.doi.org/10.18632/aging.102190
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