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Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis

Objective: This study aimed to investigate the roles of long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) in osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in periodontitis. Methods: Differentially expressed lncRNAs and mRNAs between periodontitis periodontal lig...

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Autores principales: Liu, Yi, Liu, Chunpeng, Zhang, Ankui, Yin, Shichang, Wang, Ting, Wang, Yan, Wang, Meiming, Liu, Yixin, Ying, Qiaohui, Sun, Jinrui, Wei, Fulan, Liu, Dongxu, Wang, Chunling, Ge, Shaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710065/
https://www.ncbi.nlm.nih.gov/pubmed/31398715
http://dx.doi.org/10.18632/aging.102105
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author Liu, Yi
Liu, Chunpeng
Zhang, Ankui
Yin, Shichang
Wang, Ting
Wang, Yan
Wang, Meiming
Liu, Yixin
Ying, Qiaohui
Sun, Jinrui
Wei, Fulan
Liu, Dongxu
Wang, Chunling
Ge, Shaohua
author_facet Liu, Yi
Liu, Chunpeng
Zhang, Ankui
Yin, Shichang
Wang, Ting
Wang, Yan
Wang, Meiming
Liu, Yixin
Ying, Qiaohui
Sun, Jinrui
Wei, Fulan
Liu, Dongxu
Wang, Chunling
Ge, Shaohua
author_sort Liu, Yi
collection PubMed
description Objective: This study aimed to investigate the roles of long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) in osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in periodontitis. Methods: Differentially expressed lncRNAs and mRNAs between periodontitis periodontal ligament tissues and healthy periodontal ligament tissues were selected out using R project. PDLSCs were identified using flow cytometry. Western blot was employed to detect pathway relative proteins. Besides, targeted relationships between lncRNA and miRNA, as well as miRNA and mRNA were verified by dual luciferase reporter gene assay. Osteogenic differentiation of PDLSCs was assessed by alkaline phosphatase (ALP) staining and Alizarin Red Staining (ARS). Markers for osteoblast (Runx2, Osterix, Osteocalcin, Colla1) were detected using western blot. Results: LncRNA MEG3 and IGF1 were both down-regulated, while miR-27a-3p was up-regulated in periodontitis samples compared with healthy samples. Overexpression of MEG3 promoted osteogenic differentiation by enhancing expression of IGF1 yet suppressing expression of miRNA-27a-3p. Meanwhile, the results of ALP and ARS staining indicated that up-regulation of lncRNA MEG3 or IGF1 promoted osteogenic differentiation in PDLSCs, which could be reversed with up-regulation of miRNA-27a-3p. Conclusion: Down-regulation of MEG3 suppressed osteogenic differentiation of PDLSCs through miR-27a-3p/IGF1 axis in periodontitis.
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spelling pubmed-67100652019-09-05 Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis Liu, Yi Liu, Chunpeng Zhang, Ankui Yin, Shichang Wang, Ting Wang, Yan Wang, Meiming Liu, Yixin Ying, Qiaohui Sun, Jinrui Wei, Fulan Liu, Dongxu Wang, Chunling Ge, Shaohua Aging (Albany NY) Research Paper Objective: This study aimed to investigate the roles of long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) in osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in periodontitis. Methods: Differentially expressed lncRNAs and mRNAs between periodontitis periodontal ligament tissues and healthy periodontal ligament tissues were selected out using R project. PDLSCs were identified using flow cytometry. Western blot was employed to detect pathway relative proteins. Besides, targeted relationships between lncRNA and miRNA, as well as miRNA and mRNA were verified by dual luciferase reporter gene assay. Osteogenic differentiation of PDLSCs was assessed by alkaline phosphatase (ALP) staining and Alizarin Red Staining (ARS). Markers for osteoblast (Runx2, Osterix, Osteocalcin, Colla1) were detected using western blot. Results: LncRNA MEG3 and IGF1 were both down-regulated, while miR-27a-3p was up-regulated in periodontitis samples compared with healthy samples. Overexpression of MEG3 promoted osteogenic differentiation by enhancing expression of IGF1 yet suppressing expression of miRNA-27a-3p. Meanwhile, the results of ALP and ARS staining indicated that up-regulation of lncRNA MEG3 or IGF1 promoted osteogenic differentiation in PDLSCs, which could be reversed with up-regulation of miRNA-27a-3p. Conclusion: Down-regulation of MEG3 suppressed osteogenic differentiation of PDLSCs through miR-27a-3p/IGF1 axis in periodontitis. Impact Journals 2019-08-09 /pmc/articles/PMC6710065/ /pubmed/31398715 http://dx.doi.org/10.18632/aging.102105 Text en Copyright © 2019 Liu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Liu, Yi
Liu, Chunpeng
Zhang, Ankui
Yin, Shichang
Wang, Ting
Wang, Yan
Wang, Meiming
Liu, Yixin
Ying, Qiaohui
Sun, Jinrui
Wei, Fulan
Liu, Dongxu
Wang, Chunling
Ge, Shaohua
Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis
title Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis
title_full Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis
title_fullStr Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis
title_full_unstemmed Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis
title_short Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis
title_sort down-regulation of long non-coding rna meg3 suppresses osteogenic differentiation of periodontal ligament stem cells (pdlscs) through mir-27a-3p/igf1 axis in periodontitis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710065/
https://www.ncbi.nlm.nih.gov/pubmed/31398715
http://dx.doi.org/10.18632/aging.102105
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