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Single-Input Regulatory Cascade for in vivo Removal of the Solubility Tag in Fusion Recombinant Proteins Produced by Escherichia coli
Solubility tags are commonly fused to target recombinant proteins to enhance their solubility and stability. In general, these protein tags must be removed to avoid misfolding of the partner protein and to allow for downstream applications. Nevertheless, in vitro tag removal increases process comple...
Autores principales: | Silva, Filipe S. R., Santos, Sara P. O., Meyer, Roberto, Alcantara-Neves, Neuza M., Pinheiro, Carina S., Pacheco, Luis G. C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710347/ https://www.ncbi.nlm.nih.gov/pubmed/31482090 http://dx.doi.org/10.3389/fbioe.2019.00200 |
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