Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is...

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Detalles Bibliográficos
Autores principales: Wosen, Jonathan E., Ilstad-Minnihan, Alexandra, Co, Julia Y., Jiang, Wei, Mukhopadhyay, Dhriti, Fernandez-Becker, Nielsen Q., Kuo, Calvin J., Amieva, Manuel R., Mellins, Elizabeth D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710476/
https://www.ncbi.nlm.nih.gov/pubmed/31481960
http://dx.doi.org/10.3389/fimmu.2019.01970
Descripción
Sumario:The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.

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