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Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines

Contamination of dental unit waterlines (DUWL) with heterotrophic bacteria can cause problems in immune compromised patients (aged, tumor and organ transplantation-patients). We focused on the use of low-concentrated ozonized water (OZW) as the biofilm formation restraint system for DUWL. Here, we e...

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Autores principales: Okubo, Keisuke, Ito, Takashi, Shiota, Yasuyoshi, Kawata, Yusuke, Yamamoto, Tadashi, Takashiba, Shogo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710486/
https://www.ncbi.nlm.nih.gov/pubmed/31463403
http://dx.doi.org/10.1016/j.heliyon.2019.e02306
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author Okubo, Keisuke
Ito, Takashi
Shiota, Yasuyoshi
Kawata, Yusuke
Yamamoto, Tadashi
Takashiba, Shogo
author_facet Okubo, Keisuke
Ito, Takashi
Shiota, Yasuyoshi
Kawata, Yusuke
Yamamoto, Tadashi
Takashiba, Shogo
author_sort Okubo, Keisuke
collection PubMed
description Contamination of dental unit waterlines (DUWL) with heterotrophic bacteria can cause problems in immune compromised patients (aged, tumor and organ transplantation-patients). We focused on the use of low-concentrated ozonized water (OZW) as the biofilm formation restraint system for DUWL. Here, we examined the effects of low-concentrated OZW on the growth of bacteria and related biofilm formation and harmfulness to dental unit components (DUCs) in vitro. OBJECTIVES: To evaluate the bactericidal effects of OZW on biofilms in DUWL and DUC in vitro. METHODS: Low-concentrated OZW (0.4 mg/L) was generated using an OZS-PTDX generator. Heterotrophic bacterial biofilms in old DUWL tubes and Candia albicans solution (control microbe) were treated with OZW for 1 h with gentle agitation before static culturing for 96 h in Reasoner's 2A liquid media. The control solutions were 0.1% cetylpyridinium chloride (CPC), chlorinated tap water (TW), and phosphate-buffered saline (PBS). Adenosine triphosphate (ATP) amounts of the microbes were measured and the biofilms of these microbes were observed using scanning electron microscopy (SEM). Moreover, surfaces of DUC soaked in OZW and TW were observed by SEM. RESULTS: The OZW reduced ATP levels in microbes to 50% compared to TW and PBS treatment, although CPC reduced it below detection limits. SEM observation revealed deformation of microbes cultured with OZW, whereas no changes were seen on DUC surfaces. CONCLUSIONS: Low-concentrated OZW is bactericidal against heterotrophic bacteria biofilms and it is not harmful to DUC, suggesting that it might be useful in preventing DUWL contamination.
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spelling pubmed-67104862019-08-28 Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines Okubo, Keisuke Ito, Takashi Shiota, Yasuyoshi Kawata, Yusuke Yamamoto, Tadashi Takashiba, Shogo Heliyon Article Contamination of dental unit waterlines (DUWL) with heterotrophic bacteria can cause problems in immune compromised patients (aged, tumor and organ transplantation-patients). We focused on the use of low-concentrated ozonized water (OZW) as the biofilm formation restraint system for DUWL. Here, we examined the effects of low-concentrated OZW on the growth of bacteria and related biofilm formation and harmfulness to dental unit components (DUCs) in vitro. OBJECTIVES: To evaluate the bactericidal effects of OZW on biofilms in DUWL and DUC in vitro. METHODS: Low-concentrated OZW (0.4 mg/L) was generated using an OZS-PTDX generator. Heterotrophic bacterial biofilms in old DUWL tubes and Candia albicans solution (control microbe) were treated with OZW for 1 h with gentle agitation before static culturing for 96 h in Reasoner's 2A liquid media. The control solutions were 0.1% cetylpyridinium chloride (CPC), chlorinated tap water (TW), and phosphate-buffered saline (PBS). Adenosine triphosphate (ATP) amounts of the microbes were measured and the biofilms of these microbes were observed using scanning electron microscopy (SEM). Moreover, surfaces of DUC soaked in OZW and TW were observed by SEM. RESULTS: The OZW reduced ATP levels in microbes to 50% compared to TW and PBS treatment, although CPC reduced it below detection limits. SEM observation revealed deformation of microbes cultured with OZW, whereas no changes were seen on DUC surfaces. CONCLUSIONS: Low-concentrated OZW is bactericidal against heterotrophic bacteria biofilms and it is not harmful to DUC, suggesting that it might be useful in preventing DUWL contamination. Elsevier 2019-08-21 /pmc/articles/PMC6710486/ /pubmed/31463403 http://dx.doi.org/10.1016/j.heliyon.2019.e02306 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Okubo, Keisuke
Ito, Takashi
Shiota, Yasuyoshi
Kawata, Yusuke
Yamamoto, Tadashi
Takashiba, Shogo
Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines
title Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines
title_full Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines
title_fullStr Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines
title_full_unstemmed Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines
title_short Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines
title_sort effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710486/
https://www.ncbi.nlm.nih.gov/pubmed/31463403
http://dx.doi.org/10.1016/j.heliyon.2019.e02306
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