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Mixed-trophies biofilm cultivation in capillary reactors

The biocatalytic application of photoautotrophic organisms is a promising alternative for the production of biofuels and value-added compounds as they do not rely on carbohydrates as a source of carbon, electrons, and energy. Although the photoautotrophic organisms hold potential for the development...

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Autores principales: Heuschkel, Ingeborg, Hoschek, Anna, Schmid, Andreas, Bühler, Bruno, Karande, Rohan, Bühler, Katja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710554/
https://www.ncbi.nlm.nih.gov/pubmed/31467861
http://dx.doi.org/10.1016/j.mex.2019.07.021
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author Heuschkel, Ingeborg
Hoschek, Anna
Schmid, Andreas
Bühler, Bruno
Karande, Rohan
Bühler, Katja
author_facet Heuschkel, Ingeborg
Hoschek, Anna
Schmid, Andreas
Bühler, Bruno
Karande, Rohan
Bühler, Katja
author_sort Heuschkel, Ingeborg
collection PubMed
description The biocatalytic application of photoautotrophic organisms is a promising alternative for the production of biofuels and value-added compounds as they do not rely on carbohydrates as a source of carbon, electrons, and energy. Although the photoautotrophic organisms hold potential for the development of sustainable processes, suitable reactor concepts that allow high cell density (HCD) cultivation of photoautotrophic microorganisms are limited. Such reactors need a high surface to volume ratio to enhance light availability. Furthermore, the accumulation of high oxygen concentrations as a consequence of oxygenic photosynthesis, and its inhibitory effect on cell growth needs to be prevented. Here, we present a method for HCD cultivation of oxygenic phototrophs based on the co-cultivation of different trophies in a biofilm format to avoid high oxygen partial-pressure and attain HCDs of up to 51.8 g(BDW) L(−1) on a lab scale. In this article, we show: • A robust method for mixed trophies biofilm cultivation in capillary reactors; • Set-up and operation of a biofilm capillary reactor; • A method to quantify oxygen in the continuous biofilm capillary reactor.
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spelling pubmed-67105542019-08-29 Mixed-trophies biofilm cultivation in capillary reactors Heuschkel, Ingeborg Hoschek, Anna Schmid, Andreas Bühler, Bruno Karande, Rohan Bühler, Katja MethodsX Agricultural and Biological Science The biocatalytic application of photoautotrophic organisms is a promising alternative for the production of biofuels and value-added compounds as they do not rely on carbohydrates as a source of carbon, electrons, and energy. Although the photoautotrophic organisms hold potential for the development of sustainable processes, suitable reactor concepts that allow high cell density (HCD) cultivation of photoautotrophic microorganisms are limited. Such reactors need a high surface to volume ratio to enhance light availability. Furthermore, the accumulation of high oxygen concentrations as a consequence of oxygenic photosynthesis, and its inhibitory effect on cell growth needs to be prevented. Here, we present a method for HCD cultivation of oxygenic phototrophs based on the co-cultivation of different trophies in a biofilm format to avoid high oxygen partial-pressure and attain HCDs of up to 51.8 g(BDW) L(−1) on a lab scale. In this article, we show: • A robust method for mixed trophies biofilm cultivation in capillary reactors; • Set-up and operation of a biofilm capillary reactor; • A method to quantify oxygen in the continuous biofilm capillary reactor. Elsevier 2019-07-25 /pmc/articles/PMC6710554/ /pubmed/31467861 http://dx.doi.org/10.1016/j.mex.2019.07.021 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Agricultural and Biological Science
Heuschkel, Ingeborg
Hoschek, Anna
Schmid, Andreas
Bühler, Bruno
Karande, Rohan
Bühler, Katja
Mixed-trophies biofilm cultivation in capillary reactors
title Mixed-trophies biofilm cultivation in capillary reactors
title_full Mixed-trophies biofilm cultivation in capillary reactors
title_fullStr Mixed-trophies biofilm cultivation in capillary reactors
title_full_unstemmed Mixed-trophies biofilm cultivation in capillary reactors
title_short Mixed-trophies biofilm cultivation in capillary reactors
title_sort mixed-trophies biofilm cultivation in capillary reactors
topic Agricultural and Biological Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710554/
https://www.ncbi.nlm.nih.gov/pubmed/31467861
http://dx.doi.org/10.1016/j.mex.2019.07.021
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