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Decreased Expression of Urinary Mammalian Target of Rapamycin mRNA Is Related to Chronic Renal Fibrosis in IgAN

BACKGROUND: Renal fibrosis is a common outcome of all pathological types of chronic kidney disease (CKD). However, the noninvasive detection of renal fibrosis remains a challenge. METHODS: We collected urine samples from 154 biopsy-proven IgA nephropathy (IgAN) patients and 61 healthy controls. The...

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Autores principales: Cao, Yuhan, Wang, Yuwei, Liu, Yinhua, Zhu, Xinjian, Zhang, Guifa, Wang, Sufen, Chen, Xiaomei, Liu, Daoqin, Fu, Cong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710774/
https://www.ncbi.nlm.nih.gov/pubmed/31485275
http://dx.doi.org/10.1155/2019/2424751
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author Cao, Yuhan
Wang, Yuwei
Liu, Yinhua
Zhu, Xinjian
Zhang, Guifa
Wang, Sufen
Chen, Xiaomei
Liu, Daoqin
Fu, Cong
author_facet Cao, Yuhan
Wang, Yuwei
Liu, Yinhua
Zhu, Xinjian
Zhang, Guifa
Wang, Sufen
Chen, Xiaomei
Liu, Daoqin
Fu, Cong
author_sort Cao, Yuhan
collection PubMed
description BACKGROUND: Renal fibrosis is a common outcome of all pathological types of chronic kidney disease (CKD). However, the noninvasive detection of renal fibrosis remains a challenge. METHODS: We collected urine samples from 154 biopsy-proven IgA nephropathy (IgAN) patients and 61 healthy controls. The expression of mTOR was measured and the correlation with renal function parameter and pathological indicators. The receiver operating characteristic (ROC) curve for the diagnosis of IgAN and renal fibrosis was calculated. RESULTS: The urinary mammalian target of rapamycin (mTOR) expression was decreased in IgAN patients. The expression of mTOR was correlated with serum creatinine, blood urea nitrogen, estimated glomerular filtration rate, 24 h proteinuria, and cystatin C. Further, the urinary mTOR expression was significantly decreased in severe renal fibrosis patients compared with mild or moderate renal fibrosis patients. Urinary mTOR expression was correlated with score of tubulointerstitial fibrosis (TIF) and score of glomerular sclerosis. The ROC curve showed that mTOR can diagnose IgAN at a cut-off value of 0.930 with the sensitivity of 90.2% and specificity of 73.8% and renal fibrosis at a cut-off value of 0.301 with the sensitivity of 71.7% and specificity of 64.8%. CONCLUSION: Urinary mTOR mRNA expression was a potential biomarker for diagnosis of IgAN and renal fibrosis in IgAN patients.
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spelling pubmed-67107742019-09-04 Decreased Expression of Urinary Mammalian Target of Rapamycin mRNA Is Related to Chronic Renal Fibrosis in IgAN Cao, Yuhan Wang, Yuwei Liu, Yinhua Zhu, Xinjian Zhang, Guifa Wang, Sufen Chen, Xiaomei Liu, Daoqin Fu, Cong Dis Markers Research Article BACKGROUND: Renal fibrosis is a common outcome of all pathological types of chronic kidney disease (CKD). However, the noninvasive detection of renal fibrosis remains a challenge. METHODS: We collected urine samples from 154 biopsy-proven IgA nephropathy (IgAN) patients and 61 healthy controls. The expression of mTOR was measured and the correlation with renal function parameter and pathological indicators. The receiver operating characteristic (ROC) curve for the diagnosis of IgAN and renal fibrosis was calculated. RESULTS: The urinary mammalian target of rapamycin (mTOR) expression was decreased in IgAN patients. The expression of mTOR was correlated with serum creatinine, blood urea nitrogen, estimated glomerular filtration rate, 24 h proteinuria, and cystatin C. Further, the urinary mTOR expression was significantly decreased in severe renal fibrosis patients compared with mild or moderate renal fibrosis patients. Urinary mTOR expression was correlated with score of tubulointerstitial fibrosis (TIF) and score of glomerular sclerosis. The ROC curve showed that mTOR can diagnose IgAN at a cut-off value of 0.930 with the sensitivity of 90.2% and specificity of 73.8% and renal fibrosis at a cut-off value of 0.301 with the sensitivity of 71.7% and specificity of 64.8%. CONCLUSION: Urinary mTOR mRNA expression was a potential biomarker for diagnosis of IgAN and renal fibrosis in IgAN patients. Hindawi 2019-08-14 /pmc/articles/PMC6710774/ /pubmed/31485275 http://dx.doi.org/10.1155/2019/2424751 Text en Copyright © 2019 Yuhan Cao et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cao, Yuhan
Wang, Yuwei
Liu, Yinhua
Zhu, Xinjian
Zhang, Guifa
Wang, Sufen
Chen, Xiaomei
Liu, Daoqin
Fu, Cong
Decreased Expression of Urinary Mammalian Target of Rapamycin mRNA Is Related to Chronic Renal Fibrosis in IgAN
title Decreased Expression of Urinary Mammalian Target of Rapamycin mRNA Is Related to Chronic Renal Fibrosis in IgAN
title_full Decreased Expression of Urinary Mammalian Target of Rapamycin mRNA Is Related to Chronic Renal Fibrosis in IgAN
title_fullStr Decreased Expression of Urinary Mammalian Target of Rapamycin mRNA Is Related to Chronic Renal Fibrosis in IgAN
title_full_unstemmed Decreased Expression of Urinary Mammalian Target of Rapamycin mRNA Is Related to Chronic Renal Fibrosis in IgAN
title_short Decreased Expression of Urinary Mammalian Target of Rapamycin mRNA Is Related to Chronic Renal Fibrosis in IgAN
title_sort decreased expression of urinary mammalian target of rapamycin mrna is related to chronic renal fibrosis in igan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710774/
https://www.ncbi.nlm.nih.gov/pubmed/31485275
http://dx.doi.org/10.1155/2019/2424751
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