Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity

Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney–infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding co...

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Detalles Bibliográficos
Autores principales: Chan, Martin Chi-Wai, Cheung, Sarah K.C., Mohammad, Kirran N., Chan, Jenny C.M., Estes, Mary K., Chan, Paul K.S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2019
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711227/
https://www.ncbi.nlm.nih.gov/pubmed/31441758
http://dx.doi.org/10.3201/eid2509.190205
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author Chan, Martin Chi-Wai
Cheung, Sarah K.C.
Mohammad, Kirran N.
Chan, Jenny C.M.
Estes, Mary K.
Chan, Paul K.S.
author_facet Chan, Martin Chi-Wai
Cheung, Sarah K.C.
Mohammad, Kirran N.
Chan, Jenny C.M.
Estes, Mary K.
Chan, Paul K.S.
author_sort Chan, Martin Chi-Wai
collection PubMed
description Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney–infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding could be used to help guide infection control.
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spelling pubmed-67112272019-09-04 Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity Chan, Martin Chi-Wai Cheung, Sarah K.C. Mohammad, Kirran N. Chan, Jenny C.M. Estes, Mary K. Chan, Paul K.S. Emerg Infect Dis Dispatch Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney–infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding could be used to help guide infection control. Centers for Disease Control and Prevention 2019-09 /pmc/articles/PMC6711227/ /pubmed/31441758 http://dx.doi.org/10.3201/eid2509.190205 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Chan, Martin Chi-Wai
Cheung, Sarah K.C.
Mohammad, Kirran N.
Chan, Jenny C.M.
Estes, Mary K.
Chan, Paul K.S.
Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity
title Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity
title_full Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity
title_fullStr Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity
title_full_unstemmed Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity
title_short Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity
title_sort use of human intestinal enteroids to detect 
human norovirus infectivity
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711227/
https://www.ncbi.nlm.nih.gov/pubmed/31441758
http://dx.doi.org/10.3201/eid2509.190205
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