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Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test
BACKGROUND: The receptors of salmon calcitonin, located on certain areas of the brain such as the periaqueductal gray matter, are responsible for pain modulation. AIMS: The effects of intracerebroventricular injection of salmon calcitonin on the behavioral response to pain and on the levels of monoa...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Galenos Publishing
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711249/ https://www.ncbi.nlm.nih.gov/pubmed/31218879 http://dx.doi.org/10.4274/balkanmedj.galenos.2019.2018.12.88 |
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author | Rahimi, Kaveh Sajedianfard, Javad Owji, Ali Akbar |
author_facet | Rahimi, Kaveh Sajedianfard, Javad Owji, Ali Akbar |
author_sort | Rahimi, Kaveh |
collection | PubMed |
description | BACKGROUND: The receptors of salmon calcitonin, located on certain areas of the brain such as the periaqueductal gray matter, are responsible for pain modulation. AIMS: The effects of intracerebroventricular injection of salmon calcitonin on the behavioral response to pain and on the levels of monoamines in the periaqueductal gray were explored using a biphasic animal model of pain. STUDY DESIGN: Animal experiment. METHODS: A total of 45 male rats were divided into four groups (n=6). Salmon calcitonin was injected into the lateral ventricle of the brain (1.5 nmol, with a volume of 5 μL). After 20 min, 2.5% formalin was subcutaneously injected into the right leg claw, and pain behavior was recorded on a numerical basis. At the time of the formalin test, the periaqueductal gray area was microdialized. High-performance liquid chromatography method was used to gauge the levels of monoamines and their metabolites. RESULTS: Intracerebroventricular injections of salmon calcitonin resulted in pain reduction in the formalin test (p<0.05). The dialysate concentrations of serotonin, dopamine, norepinephrine, 5-hydroxyindoleacetic acid, 3,4-dihydroxyphenylacetic, and 4-hydroxy-3-methoxyphenylglycol increased in the periaqueductal gray area in different phases of the formalin pain test (p<0.05). CONCLUSION: Salmon calcitonin reduced pain by increasing the concentrations of monoamines and the metabolites derived from them in the periaqueductal gray area. |
format | Online Article Text |
id | pubmed-6711249 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Galenos Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-67112492019-09-06 Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test Rahimi, Kaveh Sajedianfard, Javad Owji, Ali Akbar Balkan Med J Original Article BACKGROUND: The receptors of salmon calcitonin, located on certain areas of the brain such as the periaqueductal gray matter, are responsible for pain modulation. AIMS: The effects of intracerebroventricular injection of salmon calcitonin on the behavioral response to pain and on the levels of monoamines in the periaqueductal gray were explored using a biphasic animal model of pain. STUDY DESIGN: Animal experiment. METHODS: A total of 45 male rats were divided into four groups (n=6). Salmon calcitonin was injected into the lateral ventricle of the brain (1.5 nmol, with a volume of 5 μL). After 20 min, 2.5% formalin was subcutaneously injected into the right leg claw, and pain behavior was recorded on a numerical basis. At the time of the formalin test, the periaqueductal gray area was microdialized. High-performance liquid chromatography method was used to gauge the levels of monoamines and their metabolites. RESULTS: Intracerebroventricular injections of salmon calcitonin resulted in pain reduction in the formalin test (p<0.05). The dialysate concentrations of serotonin, dopamine, norepinephrine, 5-hydroxyindoleacetic acid, 3,4-dihydroxyphenylacetic, and 4-hydroxy-3-methoxyphenylglycol increased in the periaqueductal gray area in different phases of the formalin pain test (p<0.05). CONCLUSION: Salmon calcitonin reduced pain by increasing the concentrations of monoamines and the metabolites derived from them in the periaqueductal gray area. Galenos Publishing 2019-09 2019-08-22 /pmc/articles/PMC6711249/ /pubmed/31218879 http://dx.doi.org/10.4274/balkanmedj.galenos.2019.2018.12.88 Text en ©Copyright 2019 by Trakya University Faculty of Medicine http://creativecommons.org/licenses/by/2.5/ The Balkan Medical Journal published by Galenos Publishing House. |
spellingShingle | Original Article Rahimi, Kaveh Sajedianfard, Javad Owji, Ali Akbar Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test |
title | Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test |
title_full | Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test |
title_fullStr | Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test |
title_full_unstemmed | Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test |
title_short | Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test |
title_sort | effects of salmon calcitonin on the concentrations of monoamines in periaqueductal gray in formalin test |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711249/ https://www.ncbi.nlm.nih.gov/pubmed/31218879 http://dx.doi.org/10.4274/balkanmedj.galenos.2019.2018.12.88 |
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