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Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test

BACKGROUND: The receptors of salmon calcitonin, located on certain areas of the brain such as the periaqueductal gray matter, are responsible for pain modulation. AIMS: The effects of intracerebroventricular injection of salmon calcitonin on the behavioral response to pain and on the levels of monoa...

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Autores principales: Rahimi, Kaveh, Sajedianfard, Javad, Owji, Ali Akbar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Galenos Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711249/
https://www.ncbi.nlm.nih.gov/pubmed/31218879
http://dx.doi.org/10.4274/balkanmedj.galenos.2019.2018.12.88
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author Rahimi, Kaveh
Sajedianfard, Javad
Owji, Ali Akbar
author_facet Rahimi, Kaveh
Sajedianfard, Javad
Owji, Ali Akbar
author_sort Rahimi, Kaveh
collection PubMed
description BACKGROUND: The receptors of salmon calcitonin, located on certain areas of the brain such as the periaqueductal gray matter, are responsible for pain modulation. AIMS: The effects of intracerebroventricular injection of salmon calcitonin on the behavioral response to pain and on the levels of monoamines in the periaqueductal gray were explored using a biphasic animal model of pain. STUDY DESIGN: Animal experiment. METHODS: A total of 45 male rats were divided into four groups (n=6). Salmon calcitonin was injected into the lateral ventricle of the brain (1.5 nmol, with a volume of 5 μL). After 20 min, 2.5% formalin was subcutaneously injected into the right leg claw, and pain behavior was recorded on a numerical basis. At the time of the formalin test, the periaqueductal gray area was microdialized. High-performance liquid chromatography method was used to gauge the levels of monoamines and their metabolites. RESULTS: Intracerebroventricular injections of salmon calcitonin resulted in pain reduction in the formalin test (p<0.05). The dialysate concentrations of serotonin, dopamine, norepinephrine, 5-hydroxyindoleacetic acid, 3,4-dihydroxyphenylacetic, and 4-hydroxy-3-methoxyphenylglycol increased in the periaqueductal gray area in different phases of the formalin pain test (p<0.05). CONCLUSION: Salmon calcitonin reduced pain by increasing the concentrations of monoamines and the metabolites derived from them in the periaqueductal gray area.
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spelling pubmed-67112492019-09-06 Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test Rahimi, Kaveh Sajedianfard, Javad Owji, Ali Akbar Balkan Med J Original Article BACKGROUND: The receptors of salmon calcitonin, located on certain areas of the brain such as the periaqueductal gray matter, are responsible for pain modulation. AIMS: The effects of intracerebroventricular injection of salmon calcitonin on the behavioral response to pain and on the levels of monoamines in the periaqueductal gray were explored using a biphasic animal model of pain. STUDY DESIGN: Animal experiment. METHODS: A total of 45 male rats were divided into four groups (n=6). Salmon calcitonin was injected into the lateral ventricle of the brain (1.5 nmol, with a volume of 5 μL). After 20 min, 2.5% formalin was subcutaneously injected into the right leg claw, and pain behavior was recorded on a numerical basis. At the time of the formalin test, the periaqueductal gray area was microdialized. High-performance liquid chromatography method was used to gauge the levels of monoamines and their metabolites. RESULTS: Intracerebroventricular injections of salmon calcitonin resulted in pain reduction in the formalin test (p<0.05). The dialysate concentrations of serotonin, dopamine, norepinephrine, 5-hydroxyindoleacetic acid, 3,4-dihydroxyphenylacetic, and 4-hydroxy-3-methoxyphenylglycol increased in the periaqueductal gray area in different phases of the formalin pain test (p<0.05). CONCLUSION: Salmon calcitonin reduced pain by increasing the concentrations of monoamines and the metabolites derived from them in the periaqueductal gray area. Galenos Publishing 2019-09 2019-08-22 /pmc/articles/PMC6711249/ /pubmed/31218879 http://dx.doi.org/10.4274/balkanmedj.galenos.2019.2018.12.88 Text en ©Copyright 2019 by Trakya University Faculty of Medicine http://creativecommons.org/licenses/by/2.5/ The Balkan Medical Journal published by Galenos Publishing House.
spellingShingle Original Article
Rahimi, Kaveh
Sajedianfard, Javad
Owji, Ali Akbar
Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test
title Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test
title_full Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test
title_fullStr Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test
title_full_unstemmed Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test
title_short Effects of Salmon Calcitonin on the Concentrations of Monoamines in Periaqueductal Gray in Formalin Test
title_sort effects of salmon calcitonin on the concentrations of monoamines in periaqueductal gray in formalin test
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711249/
https://www.ncbi.nlm.nih.gov/pubmed/31218879
http://dx.doi.org/10.4274/balkanmedj.galenos.2019.2018.12.88
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