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Identification of R249 Mutation in P53 Gene in Tumoral Tissue of Tongue Cancer

BACKGROUND: Tongue cancer is the most common malignancy of the mouth. In recent decades, reported tongue cancer incidence and mortality rates have increased all over the world while survival has not improved that sometimes is related to mutation, especially in gene P53 (such R249, R248 mutations). H...

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Autores principales: Rogha, Mehrdad, Berjis, Nezamoddin, Lajevardi, Seyed Mohammad, Alamdaran, Masoumeh, Hashemi, Seyed Mostafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711340/
https://www.ncbi.nlm.nih.gov/pubmed/31516670
http://dx.doi.org/10.4103/ijpvm.IJPVM_50_17
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author Rogha, Mehrdad
Berjis, Nezamoddin
Lajevardi, Seyed Mohammad
Alamdaran, Masoumeh
Hashemi, Seyed Mostafa
author_facet Rogha, Mehrdad
Berjis, Nezamoddin
Lajevardi, Seyed Mohammad
Alamdaran, Masoumeh
Hashemi, Seyed Mostafa
author_sort Rogha, Mehrdad
collection PubMed
description BACKGROUND: Tongue cancer is the most common malignancy of the mouth. In recent decades, reported tongue cancer incidence and mortality rates have increased all over the world while survival has not improved that sometimes is related to mutation, especially in gene P53 (such R249, R248 mutations). Hence, this study aimed to identification of R249 mutation in P53 gene of tumor tissue in tongue cancer. METHODS: In a cross-sectional study, 48 patients with squamous cell carcinoma (SCC) of tongue were selected, and mutation of R249 was investigated in sample of tumors. In addition, demographic data and medical history of patients were determined and registered in a collected data form. Finally added data were entered to computer and analyzed by SPSS software. RESULTS: Polymerase-chain reaction test done on tissue samples from cancer patients showed that in a studied sample of 48 patients, 4 of them (8.3%) had R249 mutation. After selecting the codon 249 as a hotspot in oral cancer, forward and reverse primers for amplification of exon 7 were obtained from the articles. CONCLUSIONS: Considering the findings of our study, R249 mutation in P53 gene in patients with SCC is relatively high. Age and alcohol consumption were factors affecting incidence of the mutation. It is necessary to take an early treatment with a single lesion of tongue to prevent severe disease and prevent disease in patient's family with screening test and prevent cancer in future with gene therapy.
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spelling pubmed-67113402019-09-12 Identification of R249 Mutation in P53 Gene in Tumoral Tissue of Tongue Cancer Rogha, Mehrdad Berjis, Nezamoddin Lajevardi, Seyed Mohammad Alamdaran, Masoumeh Hashemi, Seyed Mostafa Int J Prev Med Original Article BACKGROUND: Tongue cancer is the most common malignancy of the mouth. In recent decades, reported tongue cancer incidence and mortality rates have increased all over the world while survival has not improved that sometimes is related to mutation, especially in gene P53 (such R249, R248 mutations). Hence, this study aimed to identification of R249 mutation in P53 gene of tumor tissue in tongue cancer. METHODS: In a cross-sectional study, 48 patients with squamous cell carcinoma (SCC) of tongue were selected, and mutation of R249 was investigated in sample of tumors. In addition, demographic data and medical history of patients were determined and registered in a collected data form. Finally added data were entered to computer and analyzed by SPSS software. RESULTS: Polymerase-chain reaction test done on tissue samples from cancer patients showed that in a studied sample of 48 patients, 4 of them (8.3%) had R249 mutation. After selecting the codon 249 as a hotspot in oral cancer, forward and reverse primers for amplification of exon 7 were obtained from the articles. CONCLUSIONS: Considering the findings of our study, R249 mutation in P53 gene in patients with SCC is relatively high. Age and alcohol consumption were factors affecting incidence of the mutation. It is necessary to take an early treatment with a single lesion of tongue to prevent severe disease and prevent disease in patient's family with screening test and prevent cancer in future with gene therapy. Wolters Kluwer - Medknow 2019-08-01 /pmc/articles/PMC6711340/ /pubmed/31516670 http://dx.doi.org/10.4103/ijpvm.IJPVM_50_17 Text en Copyright: © 2019 International Journal of Preventive Medicine http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Rogha, Mehrdad
Berjis, Nezamoddin
Lajevardi, Seyed Mohammad
Alamdaran, Masoumeh
Hashemi, Seyed Mostafa
Identification of R249 Mutation in P53 Gene in Tumoral Tissue of Tongue Cancer
title Identification of R249 Mutation in P53 Gene in Tumoral Tissue of Tongue Cancer
title_full Identification of R249 Mutation in P53 Gene in Tumoral Tissue of Tongue Cancer
title_fullStr Identification of R249 Mutation in P53 Gene in Tumoral Tissue of Tongue Cancer
title_full_unstemmed Identification of R249 Mutation in P53 Gene in Tumoral Tissue of Tongue Cancer
title_short Identification of R249 Mutation in P53 Gene in Tumoral Tissue of Tongue Cancer
title_sort identification of r249 mutation in p53 gene in tumoral tissue of tongue cancer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711340/
https://www.ncbi.nlm.nih.gov/pubmed/31516670
http://dx.doi.org/10.4103/ijpvm.IJPVM_50_17
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