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Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)

PREMISE OF THE STUDY: To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. METHODS AND RESULTS: The complete chloroplast genome of L. glauca was used t...

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Detalles Bibliográficos
Autores principales: Ye, Jun‐Wei, Li, De‐Zhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711346/
https://www.ncbi.nlm.nih.gov/pubmed/31467802
http://dx.doi.org/10.1002/aps3.1213
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author Ye, Jun‐Wei
Li, De‐Zhu
author_facet Ye, Jun‐Wei
Li, De‐Zhu
author_sort Ye, Jun‐Wei
collection PubMed
description PREMISE OF THE STUDY: To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. METHODS AND RESULTS: The complete chloroplast genome of L. glauca was used to design 74 primers for L. aggregata. Agarose gel electrophoresis and Sanger sequencing were used to select 20 polymorphic primers. These primers were then tested in 70 individuals from three populations of L. aggregata. The number of alleles ranged from two to four, and the unbiased haploid diversity index ranged from 0.457 to 0.685. Up to 17 primers successfully amplified in four other Lindera species: L. prattii, L. chunii, L. lungshengensis, and L. pulcherrima var. hemsleyana. CONCLUSIONS: The 20 primers reported here can potentially be used for future genetic studies of L. aggregata and other Lindera species.
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spelling pubmed-67113462019-08-29 Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae) Ye, Jun‐Wei Li, De‐Zhu Appl Plant Sci Primer Notes PREMISE OF THE STUDY: To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. METHODS AND RESULTS: The complete chloroplast genome of L. glauca was used to design 74 primers for L. aggregata. Agarose gel electrophoresis and Sanger sequencing were used to select 20 polymorphic primers. These primers were then tested in 70 individuals from three populations of L. aggregata. The number of alleles ranged from two to four, and the unbiased haploid diversity index ranged from 0.457 to 0.685. Up to 17 primers successfully amplified in four other Lindera species: L. prattii, L. chunii, L. lungshengensis, and L. pulcherrima var. hemsleyana. CONCLUSIONS: The 20 primers reported here can potentially be used for future genetic studies of L. aggregata and other Lindera species. John Wiley and Sons Inc. 2019-07-26 /pmc/articles/PMC6711346/ /pubmed/31467802 http://dx.doi.org/10.1002/aps3.1213 Text en © 2019 Ye and Li Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Primer Notes
Ye, Jun‐Wei
Li, De‐Zhu
Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
title Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
title_full Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
title_fullStr Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
title_full_unstemmed Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
title_short Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
title_sort development of 20 chloroplast microsatellite primers in wuyao (lindera aggregata, lauraceae)
topic Primer Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711346/
https://www.ncbi.nlm.nih.gov/pubmed/31467802
http://dx.doi.org/10.1002/aps3.1213
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