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A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology

Knock-in (KI) gene targeting can be employed for a wide range of applications in stem cell research. However, vectors for KI require multiple complicated processes for construction, including multiple times of digestion/ligation steps and extensive restriction mapping, which has imposed limitations...

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Detalles Bibliográficos
Autores principales:	Yoshimatsu, Sho, Sone, Takefumi, Nakajima, Mayutaka, Sato, Tsukika, Okochi, Ryotaro, Ishikawa, Mitsuru, Nakamura, Mari, Sasaki, Erika, Shiozawa, Seiji, Okano, Hideyuki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711506/ https://www.ncbi.nlm.nih.gov/pubmed/31454364 http://dx.doi.org/10.1371/journal.pone.0221164

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