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Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean

Food contamination by cadmium (Cd) is a serious threat to human health. Thus, it is imperative to prevent Cd accumulation in staple crops like soybean. The development of low Cd accumulating cultivars is an effective solution. To this end, it is essential to identify the gene(s) controlling seed Cd...

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Autores principales: Hirata, Kaori, Takagi, Kyoko, Yamada, Tetsuya, Sayama, Takashi, Anai, Toyoaki, Kikuchi, Akio, Ishimoto, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711727/
https://www.ncbi.nlm.nih.gov/pubmed/31481844
http://dx.doi.org/10.1270/jsbbs.18091
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author Hirata, Kaori
Takagi, Kyoko
Yamada, Tetsuya
Sayama, Takashi
Anai, Toyoaki
Kikuchi, Akio
Ishimoto, Masao
author_facet Hirata, Kaori
Takagi, Kyoko
Yamada, Tetsuya
Sayama, Takashi
Anai, Toyoaki
Kikuchi, Akio
Ishimoto, Masao
author_sort Hirata, Kaori
collection PubMed
description Food contamination by cadmium (Cd) is a serious threat to human health. Thus, it is imperative to prevent Cd accumulation in staple crops like soybean. The development of low Cd accumulating cultivars is an effective solution. To this end, it is essential to identify the gene(s) controlling seed Cd accumulation. Although Glyma.09G055600 (GmHMA3) seems to be associated with Cd accumulation in soybean, it has not been established if it is responsible for seed Cd accumulation. In the present study, the effect of GmHMA3 on seed Cd accumulation in soybean was validated using three independent GmHMA3 mutants isolated from an ethyl methanesulfonate-induced soybean mutant library. Each of mutant had an amino acid substitution in GmHMA3 and segregating progenies were developed by crossing the original cultivar with each of the three mutants. The relationship between these three mutations and seed Cd accumulation was investigated. While two of them significantly increased seed Cd accumulation corresponding to previous reports of a natural missense mutation in GmHMA3, the other slightly decreased seed Cd accumulation. Overall, these results indicate that GmHMA3 is responsible for seed Cd accumulation in soybean.
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spelling pubmed-67117272019-09-03 Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean Hirata, Kaori Takagi, Kyoko Yamada, Tetsuya Sayama, Takashi Anai, Toyoaki Kikuchi, Akio Ishimoto, Masao Breed Sci Note Food contamination by cadmium (Cd) is a serious threat to human health. Thus, it is imperative to prevent Cd accumulation in staple crops like soybean. The development of low Cd accumulating cultivars is an effective solution. To this end, it is essential to identify the gene(s) controlling seed Cd accumulation. Although Glyma.09G055600 (GmHMA3) seems to be associated with Cd accumulation in soybean, it has not been established if it is responsible for seed Cd accumulation. In the present study, the effect of GmHMA3 on seed Cd accumulation in soybean was validated using three independent GmHMA3 mutants isolated from an ethyl methanesulfonate-induced soybean mutant library. Each of mutant had an amino acid substitution in GmHMA3 and segregating progenies were developed by crossing the original cultivar with each of the three mutants. The relationship between these three mutations and seed Cd accumulation was investigated. While two of them significantly increased seed Cd accumulation corresponding to previous reports of a natural missense mutation in GmHMA3, the other slightly decreased seed Cd accumulation. Overall, these results indicate that GmHMA3 is responsible for seed Cd accumulation in soybean. Japanese Society of Breeding 2019-06 2019-04-27 /pmc/articles/PMC6711727/ /pubmed/31481844 http://dx.doi.org/10.1270/jsbbs.18091 Text en Copyright © 2019 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Hirata, Kaori
Takagi, Kyoko
Yamada, Tetsuya
Sayama, Takashi
Anai, Toyoaki
Kikuchi, Akio
Ishimoto, Masao
Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean
title Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean
title_full Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean
title_fullStr Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean
title_full_unstemmed Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean
title_short Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean
title_sort isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711727/
https://www.ncbi.nlm.nih.gov/pubmed/31481844
http://dx.doi.org/10.1270/jsbbs.18091
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