Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation

Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator system adapted into hPSCs and perform RNA sequencing on cell cycle sorted hPSCs primed...

Descripción completa

Detalles Bibliográficos
Autores principales: Shcherbina, Anna, Li, Jingling, Narayanan, Cyndhavi, Greenleaf, William, Kundaje, Anshul, Chetty, Sundari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Embryonic Stem Cells/Induced Pluripotent Stem Cells
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711778/
https://www.ncbi.nlm.nih.gov/pubmed/31135093
http://dx.doi.org/10.1002/stem.3041
_version_ 1783446568164130816
author Shcherbina, Anna
Li, Jingling
Narayanan, Cyndhavi
Greenleaf, William
Kundaje, Anshul
Chetty, Sundari
author_facet Shcherbina, Anna
Li, Jingling
Narayanan, Cyndhavi
Greenleaf, William
Kundaje, Anshul
Chetty, Sundari
author_sort Shcherbina, Anna
collection PubMed
description Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator system adapted into hPSCs and perform RNA sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle‐specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs toward differentiation. stem cells 2019;37:1151–1157
format Online
Article
Text
id pubmed-6711778
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley & Sons, Inc.
record_format MEDLINE/PubMed
spelling pubmed-67117782019-10-07 Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation Shcherbina, Anna Li, Jingling Narayanan, Cyndhavi Greenleaf, William Kundaje, Anshul Chetty, Sundari Stem Cells Embryonic Stem Cells/Induced Pluripotent Stem Cells Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator system adapted into hPSCs and perform RNA sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle‐specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs toward differentiation. stem cells 2019;37:1151–1157 John Wiley & Sons, Inc. 2019-06-10 2019-09 /pmc/articles/PMC6711778/ /pubmed/31135093 http://dx.doi.org/10.1002/stem.3041 Text en ©2019 The Authors. stem cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press 2019 This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Embryonic Stem Cells/Induced Pluripotent Stem Cells
Shcherbina, Anna
Li, Jingling
Narayanan, Cyndhavi
Greenleaf, William
Kundaje, Anshul
Chetty, Sundari
Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation
title Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation
title_full Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation
title_fullStr Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation
title_full_unstemmed Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation
title_short Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation
title_sort brief report: cell cycle dynamics of human pluripotent stem cells primed for differentiation
topic Embryonic Stem Cells/Induced Pluripotent Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711778/
https://www.ncbi.nlm.nih.gov/pubmed/31135093
http://dx.doi.org/10.1002/stem.3041
work_keys_str_mv AT shcherbinaanna briefreportcellcycledynamicsofhumanpluripotentstemcellsprimedfordifferentiation
AT lijingling briefreportcellcycledynamicsofhumanpluripotentstemcellsprimedfordifferentiation
AT narayanancyndhavi briefreportcellcycledynamicsofhumanpluripotentstemcellsprimedfordifferentiation
AT greenleafwilliam briefreportcellcycledynamicsofhumanpluripotentstemcellsprimedfordifferentiation
AT kundajeanshul briefreportcellcycledynamicsofhumanpluripotentstemcellsprimedfordifferentiation
AT chettysundari briefreportcellcycledynamicsofhumanpluripotentstemcellsprimedfordifferentiation

Ejemplares similares