Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism

Human dendritic cells were differentiated from blood monocytes and treated with malondialdehyde (MDA) conjugated with human serum albumin (HSA). Autologous T cells from human plaques or blood were co-cultured with the pre-treated dendritic cells or treated directly. MDA modifications were studied by...

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Detalles Bibliográficos
Autores principales: Rahman, Mizanur, Steuer, Johnny, Gillgren, Peter, Végvári, Ákos, Liu, Anquan, Frostegård, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
PRECLINICAL RESEARCH
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712057/
https://www.ncbi.nlm.nih.gov/pubmed/31468003
http://dx.doi.org/10.1016/j.jacbts.2019.03.009
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author Rahman, Mizanur
Steuer, Johnny
Gillgren, Peter
Végvári, Ákos
Liu, Anquan
Frostegård, Johan
author_facet Rahman, Mizanur
Steuer, Johnny
Gillgren, Peter
Végvári, Ákos
Liu, Anquan
Frostegård, Johan
author_sort Rahman, Mizanur
collection PubMed
description Human dendritic cells were differentiated from blood monocytes and treated with malondialdehyde (MDA) conjugated with human serum albumin (HSA). Autologous T cells from human plaques or blood were co-cultured with the pre-treated dendritic cells or treated directly. MDA modifications were studied by mass spectrometry. MDA-HSA induced a pro-inflammatory DC-mediated T-cell activation and also a strong direct effect on T cells, inhibited by an inhibitor of oxidative stress and antibodies against MDA. Atherogenic heat shock protein-60 was strongly induced in T cells activated by MDA-HSA. Two peptide modifications in atherosclerotic patients' HSA were similar to those present in in vitro MDA-modified HSA.
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spelling pubmed-67120572019-08-29 Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism Rahman, Mizanur Steuer, Johnny Gillgren, Peter Végvári, Ákos Liu, Anquan Frostegård, Johan JACC Basic Transl Sci PRECLINICAL RESEARCH Human dendritic cells were differentiated from blood monocytes and treated with malondialdehyde (MDA) conjugated with human serum albumin (HSA). Autologous T cells from human plaques or blood were co-cultured with the pre-treated dendritic cells or treated directly. MDA modifications were studied by mass spectrometry. MDA-HSA induced a pro-inflammatory DC-mediated T-cell activation and also a strong direct effect on T cells, inhibited by an inhibitor of oxidative stress and antibodies against MDA. Atherogenic heat shock protein-60 was strongly induced in T cells activated by MDA-HSA. Two peptide modifications in atherosclerotic patients' HSA were similar to those present in in vitro MDA-modified HSA. Elsevier 2019-07-10 /pmc/articles/PMC6712057/ /pubmed/31468003 http://dx.doi.org/10.1016/j.jacbts.2019.03.009 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle PRECLINICAL RESEARCH
Rahman, Mizanur
Steuer, Johnny
Gillgren, Peter
Végvári, Ákos
Liu, Anquan
Frostegård, Johan
Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism
title Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism
title_full Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism
title_fullStr Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism
title_full_unstemmed Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism
title_short Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism
title_sort malondialdehyde conjugated with albumin induces pro-inflammatory activation of t cells isolated from human atherosclerotic plaques both directly and via dendritic cell–mediated mechanism
topic PRECLINICAL RESEARCH
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712057/
https://www.ncbi.nlm.nih.gov/pubmed/31468003
http://dx.doi.org/10.1016/j.jacbts.2019.03.009
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