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Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics
Viruses are an abundant, diverse and dynamic component of marine and terrestrial ecosystems. In the ocean, viruses play a key role in the biogeochemical cycles and controlling microbial abundance, diversity and evolution. Recent metagenomics studies assessed the structure of the viral community in t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712177/ https://www.ncbi.nlm.nih.gov/pubmed/31496997 http://dx.doi.org/10.3389/fmicb.2019.01801 |
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author | De Corte, Daniele Martínez, Joaquín Martínez Cretoiu, Mariana Silvia Takaki, Yoshihiro Nunoura, Takuro Sintes, Eva Herndl, Gerhard J. Yokokawa, Taichi |
author_facet | De Corte, Daniele Martínez, Joaquín Martínez Cretoiu, Mariana Silvia Takaki, Yoshihiro Nunoura, Takuro Sintes, Eva Herndl, Gerhard J. Yokokawa, Taichi |
author_sort | De Corte, Daniele |
collection | PubMed |
description | Viruses are an abundant, diverse and dynamic component of marine and terrestrial ecosystems. In the ocean, viruses play a key role in the biogeochemical cycles and controlling microbial abundance, diversity and evolution. Recent metagenomics studies assessed the structure of the viral community in the upper ocean. However, little is known about the compositional changes in viral communities along the deep ocean conveyor belt. To assess potential changes in the viral community in the global deep-water circulation system, water samples were collected in the core of the North Atlantic Deep Water (NADW) (∼2,500 m) and Pacific Antarctic Bottom Water (∼4,000 m). Microbial and viral abundance were evaluated by flow cytometry. Subsequently, flow cytometry was used to sort virus-like particles and next generation sequencing was applied to build DNA libraries from the sorted virus populations. The viral communities were highly diverse across different oceanic regions with high dissimilarity between samples. Only 18% of the viral protein clusters were shared between the NADW and the Pacific Antarctic Bottom Water. Few viral groups, mainly associated with uncultured environmental and uncultured Mediterranean viruses were ubiquitously distributed along the global deep-water circulation system. Thus, our results point to a few groups of widely distributed abundant viruses in addition to the presence of rare and diverse types of viruses at a local scale. |
format | Online Article Text |
id | pubmed-6712177 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67121772019-09-06 Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics De Corte, Daniele Martínez, Joaquín Martínez Cretoiu, Mariana Silvia Takaki, Yoshihiro Nunoura, Takuro Sintes, Eva Herndl, Gerhard J. Yokokawa, Taichi Front Microbiol Microbiology Viruses are an abundant, diverse and dynamic component of marine and terrestrial ecosystems. In the ocean, viruses play a key role in the biogeochemical cycles and controlling microbial abundance, diversity and evolution. Recent metagenomics studies assessed the structure of the viral community in the upper ocean. However, little is known about the compositional changes in viral communities along the deep ocean conveyor belt. To assess potential changes in the viral community in the global deep-water circulation system, water samples were collected in the core of the North Atlantic Deep Water (NADW) (∼2,500 m) and Pacific Antarctic Bottom Water (∼4,000 m). Microbial and viral abundance were evaluated by flow cytometry. Subsequently, flow cytometry was used to sort virus-like particles and next generation sequencing was applied to build DNA libraries from the sorted virus populations. The viral communities were highly diverse across different oceanic regions with high dissimilarity between samples. Only 18% of the viral protein clusters were shared between the NADW and the Pacific Antarctic Bottom Water. Few viral groups, mainly associated with uncultured environmental and uncultured Mediterranean viruses were ubiquitously distributed along the global deep-water circulation system. Thus, our results point to a few groups of widely distributed abundant viruses in addition to the presence of rare and diverse types of viruses at a local scale. Frontiers Media S.A. 2019-08-21 /pmc/articles/PMC6712177/ /pubmed/31496997 http://dx.doi.org/10.3389/fmicb.2019.01801 Text en Copyright © 2019 De Corte, Martínez Martínez, Cretoiu, Takaki, Nunoura, Sintes, Herndl and Yokokawa. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology De Corte, Daniele Martínez, Joaquín Martínez Cretoiu, Mariana Silvia Takaki, Yoshihiro Nunoura, Takuro Sintes, Eva Herndl, Gerhard J. Yokokawa, Taichi Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics |
title | Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics |
title_full | Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics |
title_fullStr | Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics |
title_full_unstemmed | Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics |
title_short | Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics |
title_sort | viral communities in the global deep ocean conveyor belt assessed by targeted viromics |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712177/ https://www.ncbi.nlm.nih.gov/pubmed/31496997 http://dx.doi.org/10.3389/fmicb.2019.01801 |
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