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Deformed wing virus type A, a major honey bee pathogen, is vectored by the mite Varroa destructor in a non-propagative manner

Honey bees, the primary managed insect pollinator, suffer considerable losses due to Deformed wing virus (DWV), an RNA virus vectored by the mite Varroa destructor. Mite vectoring has resulted in the emergence of virulent DWV variants. The basis for such changes in DWV is poorly understood. Most imp...

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Autores principales: Posada-Florez, Francisco, Childers, Anna K., Heerman, Matthew C., Egekwu, Noble I., Cook, Steven C., Chen, Yanping, Evans, Jay D., Ryabov, Eugene V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712216/
https://www.ncbi.nlm.nih.gov/pubmed/31455863
http://dx.doi.org/10.1038/s41598-019-47447-3
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author Posada-Florez, Francisco
Childers, Anna K.
Heerman, Matthew C.
Egekwu, Noble I.
Cook, Steven C.
Chen, Yanping
Evans, Jay D.
Ryabov, Eugene V.
author_facet Posada-Florez, Francisco
Childers, Anna K.
Heerman, Matthew C.
Egekwu, Noble I.
Cook, Steven C.
Chen, Yanping
Evans, Jay D.
Ryabov, Eugene V.
author_sort Posada-Florez, Francisco
collection PubMed
description Honey bees, the primary managed insect pollinator, suffer considerable losses due to Deformed wing virus (DWV), an RNA virus vectored by the mite Varroa destructor. Mite vectoring has resulted in the emergence of virulent DWV variants. The basis for such changes in DWV is poorly understood. Most importantly, it remains unclear whether replication of DWV occurs in the mite. In this study, we exposed Varroa mites to DWV type A via feeding on artificially infected honey bees. A significant, 357-fold increase in DWV load was observed in these mites after 2 days. However, after 8 additional days of passage on honey bee pupae with low viral loads, the DWV load dropped by 29-fold. This decrease significantly reduced the mites’ ability to transmit DWV to honey bees. Notably, negative-strand DWV RNA, which could indicate viral replication, was detected only in mites collected from pupae with high DWV levels but not in the passaged mites. We also found that Varroa mites contain honey bee mRNAs, consistent with the acquisition of honey bee cells which would additionally contain DWV replication complexes with negative-strand DWV RNA. We propose that transmission of DWV type A by Varroa mites occurs in a non-propagative manner.
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spelling pubmed-67122162019-09-13 Deformed wing virus type A, a major honey bee pathogen, is vectored by the mite Varroa destructor in a non-propagative manner Posada-Florez, Francisco Childers, Anna K. Heerman, Matthew C. Egekwu, Noble I. Cook, Steven C. Chen, Yanping Evans, Jay D. Ryabov, Eugene V. Sci Rep Article Honey bees, the primary managed insect pollinator, suffer considerable losses due to Deformed wing virus (DWV), an RNA virus vectored by the mite Varroa destructor. Mite vectoring has resulted in the emergence of virulent DWV variants. The basis for such changes in DWV is poorly understood. Most importantly, it remains unclear whether replication of DWV occurs in the mite. In this study, we exposed Varroa mites to DWV type A via feeding on artificially infected honey bees. A significant, 357-fold increase in DWV load was observed in these mites after 2 days. However, after 8 additional days of passage on honey bee pupae with low viral loads, the DWV load dropped by 29-fold. This decrease significantly reduced the mites’ ability to transmit DWV to honey bees. Notably, negative-strand DWV RNA, which could indicate viral replication, was detected only in mites collected from pupae with high DWV levels but not in the passaged mites. We also found that Varroa mites contain honey bee mRNAs, consistent with the acquisition of honey bee cells which would additionally contain DWV replication complexes with negative-strand DWV RNA. We propose that transmission of DWV type A by Varroa mites occurs in a non-propagative manner. Nature Publishing Group UK 2019-08-27 /pmc/articles/PMC6712216/ /pubmed/31455863 http://dx.doi.org/10.1038/s41598-019-47447-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Posada-Florez, Francisco
Childers, Anna K.
Heerman, Matthew C.
Egekwu, Noble I.
Cook, Steven C.
Chen, Yanping
Evans, Jay D.
Ryabov, Eugene V.
Deformed wing virus type A, a major honey bee pathogen, is vectored by the mite Varroa destructor in a non-propagative manner
title Deformed wing virus type A, a major honey bee pathogen, is vectored by the mite Varroa destructor in a non-propagative manner
title_full Deformed wing virus type A, a major honey bee pathogen, is vectored by the mite Varroa destructor in a non-propagative manner
title_fullStr Deformed wing virus type A, a major honey bee pathogen, is vectored by the mite Varroa destructor in a non-propagative manner
title_full_unstemmed Deformed wing virus type A, a major honey bee pathogen, is vectored by the mite Varroa destructor in a non-propagative manner
title_short Deformed wing virus type A, a major honey bee pathogen, is vectored by the mite Varroa destructor in a non-propagative manner
title_sort deformed wing virus type a, a major honey bee pathogen, is vectored by the mite varroa destructor in a non-propagative manner
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712216/
https://www.ncbi.nlm.nih.gov/pubmed/31455863
http://dx.doi.org/10.1038/s41598-019-47447-3
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