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Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats

The aim of the present study was to explore the wound healing effect of Tcf3 in rat bone marrow mesenchymal stem cells (BMSCs) and their effects on wound healing. Epidermal growth factor (EGF) and fibroblast growth factors (FGFs) were used to induce BMSCs differentiation into epithelial-like cells....

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Autores principales: He, Bin, Chen, Jia, Liu, Liang, Wang, Hao, Wang, Shaohua, Li, Ping, Zhou, Jianda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712438/
https://www.ncbi.nlm.nih.gov/pubmed/31085716
http://dx.doi.org/10.1042/BSR20180369
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author He, Bin
Chen, Jia
Liu, Liang
Wang, Hao
Wang, Shaohua
Li, Ping
Zhou, Jianda
author_facet He, Bin
Chen, Jia
Liu, Liang
Wang, Hao
Wang, Shaohua
Li, Ping
Zhou, Jianda
author_sort He, Bin
collection PubMed
description The aim of the present study was to explore the wound healing effect of Tcf3 in rat bone marrow mesenchymal stem cells (BMSCs) and their effects on wound healing. Epidermal growth factor (EGF) and fibroblast growth factors (FGFs) were used to induce BMSCs differentiation into epithelial-like cells. Western Blotting analysis and RT-qPCR were performed to assess the expression levels of Tcf3 and the markers of epithelial-like cells, such as Cytokeratin-18 (CK-18), CK-19 and P63. Cell counting kit-8 (CCK-8) and clone formation assay were carried out to detect cell viability. Immunohistochemistry and HE staining were used to assess the level of Tcf3 protein and skin repair degree, respectively. Rat wound healing model was built to evaluate the effects of BMSCs with altered expression of Tcf3 on wound healing. Results showed that EGF and FGFs stimulation increased the expression of CK-18, CK-19 and P63, improved BMSCs viability, but decreased the expression of Tcf3. Knockdown of Tcf3 in BMSCs increased CK-18, CK-19 and P63 expression and improved cell proliferation, as well as accelerated wound healing process. Moreover, inhibition of Wnt/β-catenin signaling weakened the effect of Tcf3 down-regulation on BMSCs proliferation enhancement. And inhibition of Notch1 signaling impeded the epithelial-like cell differentiation of BMSCs induced by Tcf3 down-regulation. Our study reveals that knockdown of Tcf3 enhances the wound healing process of BMSCs in rat, which provides new approach for accelerating skin regeneration.
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spelling pubmed-67124382019-09-06 Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats He, Bin Chen, Jia Liu, Liang Wang, Hao Wang, Shaohua Li, Ping Zhou, Jianda Biosci Rep Research Articles The aim of the present study was to explore the wound healing effect of Tcf3 in rat bone marrow mesenchymal stem cells (BMSCs) and their effects on wound healing. Epidermal growth factor (EGF) and fibroblast growth factors (FGFs) were used to induce BMSCs differentiation into epithelial-like cells. Western Blotting analysis and RT-qPCR were performed to assess the expression levels of Tcf3 and the markers of epithelial-like cells, such as Cytokeratin-18 (CK-18), CK-19 and P63. Cell counting kit-8 (CCK-8) and clone formation assay were carried out to detect cell viability. Immunohistochemistry and HE staining were used to assess the level of Tcf3 protein and skin repair degree, respectively. Rat wound healing model was built to evaluate the effects of BMSCs with altered expression of Tcf3 on wound healing. Results showed that EGF and FGFs stimulation increased the expression of CK-18, CK-19 and P63, improved BMSCs viability, but decreased the expression of Tcf3. Knockdown of Tcf3 in BMSCs increased CK-18, CK-19 and P63 expression and improved cell proliferation, as well as accelerated wound healing process. Moreover, inhibition of Wnt/β-catenin signaling weakened the effect of Tcf3 down-regulation on BMSCs proliferation enhancement. And inhibition of Notch1 signaling impeded the epithelial-like cell differentiation of BMSCs induced by Tcf3 down-regulation. Our study reveals that knockdown of Tcf3 enhances the wound healing process of BMSCs in rat, which provides new approach for accelerating skin regeneration. Portland Press Ltd. 2019-08-28 /pmc/articles/PMC6712438/ /pubmed/31085716 http://dx.doi.org/10.1042/BSR20180369 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
He, Bin
Chen, Jia
Liu, Liang
Wang, Hao
Wang, Shaohua
Li, Ping
Zhou, Jianda
Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats
title Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats
title_full Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats
title_fullStr Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats
title_full_unstemmed Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats
title_short Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats
title_sort knockdown of tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712438/
https://www.ncbi.nlm.nih.gov/pubmed/31085716
http://dx.doi.org/10.1042/BSR20180369
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