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Vitamin D-conjugated gold nanoparticles as functional carriers to enhancing osteogenic differentiation

In an aging society, bone disorders such as osteopenia, osteoporosis, and degenerative arthritis cause serious public health problems. In order to solve these problems, researchers continue to develop therapeutic agents, increase the efficacy of developed therapeutic agents, and reduce side effects....

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Autores principales: Nah, Haram, Lee, Donghyun, Heo, Min, Lee, Jae Seo, Lee, Sang Jin, Heo, Dong Nyoung, Seong, Jeongmin, Lim, Ho-Nam, Lee, Yeon-Hee, Moon, Ho-Jin, Hwang, Yu-Shik, Kwon, Il Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713151/
https://www.ncbi.nlm.nih.gov/pubmed/31489055
http://dx.doi.org/10.1080/14686996.2019.1644193
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author Nah, Haram
Lee, Donghyun
Heo, Min
Lee, Jae Seo
Lee, Sang Jin
Heo, Dong Nyoung
Seong, Jeongmin
Lim, Ho-Nam
Lee, Yeon-Hee
Moon, Ho-Jin
Hwang, Yu-Shik
Kwon, Il Keun
author_facet Nah, Haram
Lee, Donghyun
Heo, Min
Lee, Jae Seo
Lee, Sang Jin
Heo, Dong Nyoung
Seong, Jeongmin
Lim, Ho-Nam
Lee, Yeon-Hee
Moon, Ho-Jin
Hwang, Yu-Shik
Kwon, Il Keun
author_sort Nah, Haram
collection PubMed
description In an aging society, bone disorders such as osteopenia, osteoporosis, and degenerative arthritis cause serious public health problems. In order to solve these problems, researchers continue to develop therapeutic agents, increase the efficacy of developed therapeutic agents, and reduce side effects. Gold nanoparticles (GNPs) are widely used in tissue engineering applications as biosensors, drug delivery carriers, and bioactive materials. Their special surface property enables easy conjugation with ligands including functional groups such as thiols, phosphines, and amines. This creates an attractive advantage to GNPs for use in the bone tissue engineering field. However, GNPs alone are limited in their biological effects. In this study, we used thiol-PEG-vitamin D (SPVD) to conjugate vitamin D, an essential nutrient critical for maintaining normal skeletal homeostasis, to GNPs. To characterize vitamin D-conjugated GNPs (VGNPs), field emission transmission electron microscopy, energy dispersive X-ray spectroscopy, dynamic light scattering, and ultraviolet/visible absorption analysis were carried out. The developed VGNPs were well bound through the thiol groups between GNPs and vitamin D, and were fabricated in size of 60 nm. Moreover, to demonstrate VGNPs osteogenic differentiation effect, various assays were carried out through cell viability test, alkaline phosphatase assay, calcium deposition assay, real-time polymerase chain reaction, and immunofluorescence staining. As a result, the fabricated VGNPs were found to effectively enhance osteogenic differentiation of human adipose-derived stem cells (hADSCs) in vitro. Based on these results, VGNPs can be utilized as functional nanomaterials for bone regeneration in the tissue engineering field.
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spelling pubmed-67131512019-09-05 Vitamin D-conjugated gold nanoparticles as functional carriers to enhancing osteogenic differentiation Nah, Haram Lee, Donghyun Heo, Min Lee, Jae Seo Lee, Sang Jin Heo, Dong Nyoung Seong, Jeongmin Lim, Ho-Nam Lee, Yeon-Hee Moon, Ho-Jin Hwang, Yu-Shik Kwon, Il Keun Sci Technol Adv Mater Bio-Inspired and Biomedical Materials In an aging society, bone disorders such as osteopenia, osteoporosis, and degenerative arthritis cause serious public health problems. In order to solve these problems, researchers continue to develop therapeutic agents, increase the efficacy of developed therapeutic agents, and reduce side effects. Gold nanoparticles (GNPs) are widely used in tissue engineering applications as biosensors, drug delivery carriers, and bioactive materials. Their special surface property enables easy conjugation with ligands including functional groups such as thiols, phosphines, and amines. This creates an attractive advantage to GNPs for use in the bone tissue engineering field. However, GNPs alone are limited in their biological effects. In this study, we used thiol-PEG-vitamin D (SPVD) to conjugate vitamin D, an essential nutrient critical for maintaining normal skeletal homeostasis, to GNPs. To characterize vitamin D-conjugated GNPs (VGNPs), field emission transmission electron microscopy, energy dispersive X-ray spectroscopy, dynamic light scattering, and ultraviolet/visible absorption analysis were carried out. The developed VGNPs were well bound through the thiol groups between GNPs and vitamin D, and were fabricated in size of 60 nm. Moreover, to demonstrate VGNPs osteogenic differentiation effect, various assays were carried out through cell viability test, alkaline phosphatase assay, calcium deposition assay, real-time polymerase chain reaction, and immunofluorescence staining. As a result, the fabricated VGNPs were found to effectively enhance osteogenic differentiation of human adipose-derived stem cells (hADSCs) in vitro. Based on these results, VGNPs can be utilized as functional nanomaterials for bone regeneration in the tissue engineering field. Taylor & Francis 2019-07-17 /pmc/articles/PMC6713151/ /pubmed/31489055 http://dx.doi.org/10.1080/14686996.2019.1644193 Text en © 2019 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis Group. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Bio-Inspired and Biomedical Materials
Nah, Haram
Lee, Donghyun
Heo, Min
Lee, Jae Seo
Lee, Sang Jin
Heo, Dong Nyoung
Seong, Jeongmin
Lim, Ho-Nam
Lee, Yeon-Hee
Moon, Ho-Jin
Hwang, Yu-Shik
Kwon, Il Keun
Vitamin D-conjugated gold nanoparticles as functional carriers to enhancing osteogenic differentiation
title Vitamin D-conjugated gold nanoparticles as functional carriers to enhancing osteogenic differentiation
title_full Vitamin D-conjugated gold nanoparticles as functional carriers to enhancing osteogenic differentiation
title_fullStr Vitamin D-conjugated gold nanoparticles as functional carriers to enhancing osteogenic differentiation
title_full_unstemmed Vitamin D-conjugated gold nanoparticles as functional carriers to enhancing osteogenic differentiation
title_short Vitamin D-conjugated gold nanoparticles as functional carriers to enhancing osteogenic differentiation
title_sort vitamin d-conjugated gold nanoparticles as functional carriers to enhancing osteogenic differentiation
topic Bio-Inspired and Biomedical Materials
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713151/
https://www.ncbi.nlm.nih.gov/pubmed/31489055
http://dx.doi.org/10.1080/14686996.2019.1644193
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