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Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs

The importance of probiotics in swine production is widely acknowledged as crucial. However, gaps still remain in the exact roles played by probiotics in modulation of gut microbiota and immune response. This study determined the roles of probiotic Lactobacillus plantarum strain JDFM LP11in gut micr...

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Autores principales: Shin, Donghyun, Chang, Sung Yong, Bogere, Paul, Won, KyeongHye, Choi, Jae-Young, Choi, Yeon-Jae, Lee, Hak Kyo, Hur, Jin, Park, Byung-Yong, Kim, Younghoon, Heo, Jaeyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713323/
https://www.ncbi.nlm.nih.gov/pubmed/31461453
http://dx.doi.org/10.1371/journal.pone.0220843
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author Shin, Donghyun
Chang, Sung Yong
Bogere, Paul
Won, KyeongHye
Choi, Jae-Young
Choi, Yeon-Jae
Lee, Hak Kyo
Hur, Jin
Park, Byung-Yong
Kim, Younghoon
Heo, Jaeyoung
author_facet Shin, Donghyun
Chang, Sung Yong
Bogere, Paul
Won, KyeongHye
Choi, Jae-Young
Choi, Yeon-Jae
Lee, Hak Kyo
Hur, Jin
Park, Byung-Yong
Kim, Younghoon
Heo, Jaeyoung
author_sort Shin, Donghyun
collection PubMed
description The importance of probiotics in swine production is widely acknowledged as crucial. However, gaps still remain in the exact roles played by probiotics in modulation of gut microbiota and immune response. This study determined the roles of probiotic Lactobacillus plantarum strain JDFM LP11in gut microbiota modulation and immune response in weaned piglets. L. plantarum JDFM LP11 increased the population of lactic acid bacteria in feces and enhanced the development of villi in the small intestine. Metagenome analysis showed that microbial diversity and richness (Simpson, Shannon, ACE, Chao1) and the relative abundance of the Firmicutes were higher in weaned piglets fed probiotics. Five bacterial families were different in the relative abundance, especially; Prevotellaceae occupied the largest part of microbial community showed the most difference between two groups. Transcriptome analysis identified 25 differentially expressed genes using RNA-sequencing data of the ileum. Further gene ontology and immune DB analysis determined 8 genes associated with innate defense response and cytokine production. BPI, RSAD2, SLPI, LUM, OLFM4, DMBT1 and C6 genes were down-regulated by probiotic supplementation except PLA2G2A. PICRUSt analysis predicting functional profiling of microbial communities indicated branched amino acid biosynthesis and butyrate metabolism promoting gut development and health were increased by probiotics. Altogether, our data suggest that L. plantarum JDFM LP11 increases the diversity and richness in the microbial community, and attenuates the ileal immune gene expression towards gut inflammation, promoting intestinal development in weaned piglets.
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spelling pubmed-67133232019-09-04 Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs Shin, Donghyun Chang, Sung Yong Bogere, Paul Won, KyeongHye Choi, Jae-Young Choi, Yeon-Jae Lee, Hak Kyo Hur, Jin Park, Byung-Yong Kim, Younghoon Heo, Jaeyoung PLoS One Research Article The importance of probiotics in swine production is widely acknowledged as crucial. However, gaps still remain in the exact roles played by probiotics in modulation of gut microbiota and immune response. This study determined the roles of probiotic Lactobacillus plantarum strain JDFM LP11in gut microbiota modulation and immune response in weaned piglets. L. plantarum JDFM LP11 increased the population of lactic acid bacteria in feces and enhanced the development of villi in the small intestine. Metagenome analysis showed that microbial diversity and richness (Simpson, Shannon, ACE, Chao1) and the relative abundance of the Firmicutes were higher in weaned piglets fed probiotics. Five bacterial families were different in the relative abundance, especially; Prevotellaceae occupied the largest part of microbial community showed the most difference between two groups. Transcriptome analysis identified 25 differentially expressed genes using RNA-sequencing data of the ileum. Further gene ontology and immune DB analysis determined 8 genes associated with innate defense response and cytokine production. BPI, RSAD2, SLPI, LUM, OLFM4, DMBT1 and C6 genes were down-regulated by probiotic supplementation except PLA2G2A. PICRUSt analysis predicting functional profiling of microbial communities indicated branched amino acid biosynthesis and butyrate metabolism promoting gut development and health were increased by probiotics. Altogether, our data suggest that L. plantarum JDFM LP11 increases the diversity and richness in the microbial community, and attenuates the ileal immune gene expression towards gut inflammation, promoting intestinal development in weaned piglets. Public Library of Science 2019-08-28 /pmc/articles/PMC6713323/ /pubmed/31461453 http://dx.doi.org/10.1371/journal.pone.0220843 Text en © 2019 Shin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shin, Donghyun
Chang, Sung Yong
Bogere, Paul
Won, KyeongHye
Choi, Jae-Young
Choi, Yeon-Jae
Lee, Hak Kyo
Hur, Jin
Park, Byung-Yong
Kim, Younghoon
Heo, Jaeyoung
Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs
title Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs
title_full Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs
title_fullStr Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs
title_full_unstemmed Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs
title_short Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs
title_sort beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713323/
https://www.ncbi.nlm.nih.gov/pubmed/31461453
http://dx.doi.org/10.1371/journal.pone.0220843
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