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Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response
Mesenchymal stem cells (MSCs) show immunosuppressive activities and alleviate atherosclerosis (AS) formation in apolipoprotein E-knockout (apoE-KO) mice. Human amnion mesenchymal stem cells (hAMSCs), a particular population of mesenchymal stem cells, have been shown to have immunomodulatory abilitie...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713407/ https://www.ncbi.nlm.nih.gov/pubmed/31364743 http://dx.doi.org/10.3892/ijmm.2019.4286 |
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author | Wei, Xiufang Sun, Guang Zhao, Xiaoxue Wu, Qianqian Chen, Ling Xu, Yichi Pang, Xining Qi, Guoxian |
author_facet | Wei, Xiufang Sun, Guang Zhao, Xiaoxue Wu, Qianqian Chen, Ling Xu, Yichi Pang, Xining Qi, Guoxian |
author_sort | Wei, Xiufang |
collection | PubMed |
description | Mesenchymal stem cells (MSCs) show immunosuppressive activities and alleviate atherosclerosis (AS) formation in apolipoprotein E-knockout (apoE-KO) mice. Human amnion mesenchymal stem cells (hAMSCs), a particular population of mesenchymal stem cells, have been shown to have immunomodulatory abilities. The present study investigated the effects of hAMSCs treatment on early atherosclerotic plaque formation and the progression of established lesion in apoE-KO mice. In total, 36 mice were fed with a high-fat diet. Mice were subjected to hAMSCs-injection treatment simultaneously with high-fat diet (early treatment) or after 8 weeks of high-fat diet (delayed treatment). In each treatment, mice were divided into three groups: i) hAMSCs group with hAMSCs treatment; ii) PBS group injected with PBS; and iii) control group without injection. Histological results showed that the plaque area in the aortic arch of mice was significantly reduced after hAMSCs treatment in the early and delayed treatment groups. In addition, immunohistochemical analysis suggested that the accumulation of macrophages was significantly decreased after hAMSCs treatment. Similarly, the release of the pro-inflammatory cytokine tumor necrosis factor-α was also decreased, whereas the release of the anti-inflammatory cytokine interleukin-10 was increased. In addition, hAMSCs treatment suppressed the phosphorylation of p65 and inhibitor of κB-α, suggesting that NF-κB pathway was involved in the hAMSCs-mediated suppression of immune response. In conclusion, hAMSCs treatment was effective in reducing immune response, which is the one of the major causes of AS, eventually leading to a significant reduction in size of athero-sclerotic lesions. |
format | Online Article Text |
id | pubmed-6713407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-67134072019-08-31 Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response Wei, Xiufang Sun, Guang Zhao, Xiaoxue Wu, Qianqian Chen, Ling Xu, Yichi Pang, Xining Qi, Guoxian Int J Mol Med Articles Mesenchymal stem cells (MSCs) show immunosuppressive activities and alleviate atherosclerosis (AS) formation in apolipoprotein E-knockout (apoE-KO) mice. Human amnion mesenchymal stem cells (hAMSCs), a particular population of mesenchymal stem cells, have been shown to have immunomodulatory abilities. The present study investigated the effects of hAMSCs treatment on early atherosclerotic plaque formation and the progression of established lesion in apoE-KO mice. In total, 36 mice were fed with a high-fat diet. Mice were subjected to hAMSCs-injection treatment simultaneously with high-fat diet (early treatment) or after 8 weeks of high-fat diet (delayed treatment). In each treatment, mice were divided into three groups: i) hAMSCs group with hAMSCs treatment; ii) PBS group injected with PBS; and iii) control group without injection. Histological results showed that the plaque area in the aortic arch of mice was significantly reduced after hAMSCs treatment in the early and delayed treatment groups. In addition, immunohistochemical analysis suggested that the accumulation of macrophages was significantly decreased after hAMSCs treatment. Similarly, the release of the pro-inflammatory cytokine tumor necrosis factor-α was also decreased, whereas the release of the anti-inflammatory cytokine interleukin-10 was increased. In addition, hAMSCs treatment suppressed the phosphorylation of p65 and inhibitor of κB-α, suggesting that NF-κB pathway was involved in the hAMSCs-mediated suppression of immune response. In conclusion, hAMSCs treatment was effective in reducing immune response, which is the one of the major causes of AS, eventually leading to a significant reduction in size of athero-sclerotic lesions. D.A. Spandidos 2019-10 2019-07-23 /pmc/articles/PMC6713407/ /pubmed/31364743 http://dx.doi.org/10.3892/ijmm.2019.4286 Text en Copyright: © Wei et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wei, Xiufang Sun, Guang Zhao, Xiaoxue Wu, Qianqian Chen, Ling Xu, Yichi Pang, Xining Qi, Guoxian Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response |
title | Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response |
title_full | Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response |
title_fullStr | Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response |
title_full_unstemmed | Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response |
title_short | Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response |
title_sort | human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713407/ https://www.ncbi.nlm.nih.gov/pubmed/31364743 http://dx.doi.org/10.3892/ijmm.2019.4286 |
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