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Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response

Mesenchymal stem cells (MSCs) show immunosuppressive activities and alleviate atherosclerosis (AS) formation in apolipoprotein E-knockout (apoE-KO) mice. Human amnion mesenchymal stem cells (hAMSCs), a particular population of mesenchymal stem cells, have been shown to have immunomodulatory abilitie...

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Autores principales: Wei, Xiufang, Sun, Guang, Zhao, Xiaoxue, Wu, Qianqian, Chen, Ling, Xu, Yichi, Pang, Xining, Qi, Guoxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713407/
https://www.ncbi.nlm.nih.gov/pubmed/31364743
http://dx.doi.org/10.3892/ijmm.2019.4286
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author Wei, Xiufang
Sun, Guang
Zhao, Xiaoxue
Wu, Qianqian
Chen, Ling
Xu, Yichi
Pang, Xining
Qi, Guoxian
author_facet Wei, Xiufang
Sun, Guang
Zhao, Xiaoxue
Wu, Qianqian
Chen, Ling
Xu, Yichi
Pang, Xining
Qi, Guoxian
author_sort Wei, Xiufang
collection PubMed
description Mesenchymal stem cells (MSCs) show immunosuppressive activities and alleviate atherosclerosis (AS) formation in apolipoprotein E-knockout (apoE-KO) mice. Human amnion mesenchymal stem cells (hAMSCs), a particular population of mesenchymal stem cells, have been shown to have immunomodulatory abilities. The present study investigated the effects of hAMSCs treatment on early atherosclerotic plaque formation and the progression of established lesion in apoE-KO mice. In total, 36 mice were fed with a high-fat diet. Mice were subjected to hAMSCs-injection treatment simultaneously with high-fat diet (early treatment) or after 8 weeks of high-fat diet (delayed treatment). In each treatment, mice were divided into three groups: i) hAMSCs group with hAMSCs treatment; ii) PBS group injected with PBS; and iii) control group without injection. Histological results showed that the plaque area in the aortic arch of mice was significantly reduced after hAMSCs treatment in the early and delayed treatment groups. In addition, immunohistochemical analysis suggested that the accumulation of macrophages was significantly decreased after hAMSCs treatment. Similarly, the release of the pro-inflammatory cytokine tumor necrosis factor-α was also decreased, whereas the release of the anti-inflammatory cytokine interleukin-10 was increased. In addition, hAMSCs treatment suppressed the phosphorylation of p65 and inhibitor of κB-α, suggesting that NF-κB pathway was involved in the hAMSCs-mediated suppression of immune response. In conclusion, hAMSCs treatment was effective in reducing immune response, which is the one of the major causes of AS, eventually leading to a significant reduction in size of athero-sclerotic lesions.
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spelling pubmed-67134072019-08-31 Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response Wei, Xiufang Sun, Guang Zhao, Xiaoxue Wu, Qianqian Chen, Ling Xu, Yichi Pang, Xining Qi, Guoxian Int J Mol Med Articles Mesenchymal stem cells (MSCs) show immunosuppressive activities and alleviate atherosclerosis (AS) formation in apolipoprotein E-knockout (apoE-KO) mice. Human amnion mesenchymal stem cells (hAMSCs), a particular population of mesenchymal stem cells, have been shown to have immunomodulatory abilities. The present study investigated the effects of hAMSCs treatment on early atherosclerotic plaque formation and the progression of established lesion in apoE-KO mice. In total, 36 mice were fed with a high-fat diet. Mice were subjected to hAMSCs-injection treatment simultaneously with high-fat diet (early treatment) or after 8 weeks of high-fat diet (delayed treatment). In each treatment, mice were divided into three groups: i) hAMSCs group with hAMSCs treatment; ii) PBS group injected with PBS; and iii) control group without injection. Histological results showed that the plaque area in the aortic arch of mice was significantly reduced after hAMSCs treatment in the early and delayed treatment groups. In addition, immunohistochemical analysis suggested that the accumulation of macrophages was significantly decreased after hAMSCs treatment. Similarly, the release of the pro-inflammatory cytokine tumor necrosis factor-α was also decreased, whereas the release of the anti-inflammatory cytokine interleukin-10 was increased. In addition, hAMSCs treatment suppressed the phosphorylation of p65 and inhibitor of κB-α, suggesting that NF-κB pathway was involved in the hAMSCs-mediated suppression of immune response. In conclusion, hAMSCs treatment was effective in reducing immune response, which is the one of the major causes of AS, eventually leading to a significant reduction in size of athero-sclerotic lesions. D.A. Spandidos 2019-10 2019-07-23 /pmc/articles/PMC6713407/ /pubmed/31364743 http://dx.doi.org/10.3892/ijmm.2019.4286 Text en Copyright: © Wei et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wei, Xiufang
Sun, Guang
Zhao, Xiaoxue
Wu, Qianqian
Chen, Ling
Xu, Yichi
Pang, Xining
Qi, Guoxian
Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response
title Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response
title_full Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response
title_fullStr Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response
title_full_unstemmed Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response
title_short Human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response
title_sort human amnion mesenchymal stem cells attenuate atherosclerosis by modulating macrophage function to reduce immune response
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713407/
https://www.ncbi.nlm.nih.gov/pubmed/31364743
http://dx.doi.org/10.3892/ijmm.2019.4286
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