A magnetic affinity approach to identify plant GABA-binding proteins

In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable...

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Detalles Bibliográficos
Autores principales: ZOU, Jie, GUO, Jingzhe, LI, Shisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific and Technological Research Council of Turkey 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713914/
https://www.ncbi.nlm.nih.gov/pubmed/31582881
http://dx.doi.org/10.3906/biy-1901-79
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author ZOU, Jie
GUO, Jingzhe
LI, Shisheng
author_facet ZOU, Jie
GUO, Jingzhe
LI, Shisheng
author_sort ZOU, Jie
collection PubMed
description In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.
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spelling pubmed-67139142019-10-03 A magnetic affinity approach to identify plant GABA-binding proteins ZOU, Jie GUO, Jingzhe LI, Shisheng Turk J Biol Article In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants. The Scientific and Technological Research Council of Turkey 2019-08-05 /pmc/articles/PMC6713914/ /pubmed/31582881 http://dx.doi.org/10.3906/biy-1901-79 Text en Copyright © 2019 The Author(s) This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Article
ZOU, Jie
GUO, Jingzhe
LI, Shisheng
A magnetic affinity approach to identify plant GABA-binding proteins
title A magnetic affinity approach to identify plant GABA-binding proteins
title_full A magnetic affinity approach to identify plant GABA-binding proteins
title_fullStr A magnetic affinity approach to identify plant GABA-binding proteins
title_full_unstemmed A magnetic affinity approach to identify plant GABA-binding proteins
title_short A magnetic affinity approach to identify plant GABA-binding proteins
title_sort magnetic affinity approach to identify plant gaba-binding proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713914/
https://www.ncbi.nlm.nih.gov/pubmed/31582881
http://dx.doi.org/10.3906/biy-1901-79
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AT zoujie magneticaffinityapproachtoidentifyplantgababindingproteins
AT guojingzhe magneticaffinityapproachtoidentifyplantgababindingproteins
AT lishisheng magneticaffinityapproachtoidentifyplantgababindingproteins

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