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Mechanical stress alters protein O‐GlcNAc in human periodontal ligament cells
Protein O‐linked N‐acetylglucosamine (O‐GlcNAc) is a post‐translational modification of intracellular proteins that regulates several physiological and pathophysiological process, including response to various stressors. However, O‐GlcNAc's response to mechanical stress has not been investigate...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714205/ https://www.ncbi.nlm.nih.gov/pubmed/31237748 http://dx.doi.org/10.1111/jcmm.14509 |
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author | Frank, Dorottya Cser, Annamária Kolarovszki, Béla Farkas, Nelli Miseta, Attila Nagy, Tamás |
author_facet | Frank, Dorottya Cser, Annamária Kolarovszki, Béla Farkas, Nelli Miseta, Attila Nagy, Tamás |
author_sort | Frank, Dorottya |
collection | PubMed |
description | Protein O‐linked N‐acetylglucosamine (O‐GlcNAc) is a post‐translational modification of intracellular proteins that regulates several physiological and pathophysiological process, including response to various stressors. However, O‐GlcNAc's response to mechanical stress has not been investigated yet. As human periodontal ligament (PDL) cells are stimulated by compression force during orthodontic tooth movement that results in structural remodelling, in this study we investigated whether mechanical stress induces any alteration in protein O‐GlcNAc in PDL cells. In this study, PDL cells isolated from premolars extracted for orthodontic indications were exposed to 0, 1.5, 3, 7 and 14 g/cm(2) compression forces for 12 hours. Cell viability was measured by flow cytometry, and protein O‐GlcNAc was analysed by Western blot. Cellular structure and intracellular distribution of O‐GlcNAc was studied by immunofluorescence microscopy. We found that between 1.5 and 3 g/cm(2) mechanical compression, O‐GlcNAc significantly elevated; however, at higher forces O‐GlcNAc level was not increased. We also found that intracellular localization of O‐GlcNAc proteins became more centralized under 2 g/cm(2) compression force. Our results suggest that structural changes stimulated by compression forces have a significant effect on the regulation of O‐GlcNAc; thus, it might play a role in the mechanical stress adaptation of PDL cells. |
format | Online Article Text |
id | pubmed-6714205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67142052019-09-05 Mechanical stress alters protein O‐GlcNAc in human periodontal ligament cells Frank, Dorottya Cser, Annamária Kolarovszki, Béla Farkas, Nelli Miseta, Attila Nagy, Tamás J Cell Mol Med Original Articles Protein O‐linked N‐acetylglucosamine (O‐GlcNAc) is a post‐translational modification of intracellular proteins that regulates several physiological and pathophysiological process, including response to various stressors. However, O‐GlcNAc's response to mechanical stress has not been investigated yet. As human periodontal ligament (PDL) cells are stimulated by compression force during orthodontic tooth movement that results in structural remodelling, in this study we investigated whether mechanical stress induces any alteration in protein O‐GlcNAc in PDL cells. In this study, PDL cells isolated from premolars extracted for orthodontic indications were exposed to 0, 1.5, 3, 7 and 14 g/cm(2) compression forces for 12 hours. Cell viability was measured by flow cytometry, and protein O‐GlcNAc was analysed by Western blot. Cellular structure and intracellular distribution of O‐GlcNAc was studied by immunofluorescence microscopy. We found that between 1.5 and 3 g/cm(2) mechanical compression, O‐GlcNAc significantly elevated; however, at higher forces O‐GlcNAc level was not increased. We also found that intracellular localization of O‐GlcNAc proteins became more centralized under 2 g/cm(2) compression force. Our results suggest that structural changes stimulated by compression forces have a significant effect on the regulation of O‐GlcNAc; thus, it might play a role in the mechanical stress adaptation of PDL cells. John Wiley and Sons Inc. 2019-06-25 2019-09 /pmc/articles/PMC6714205/ /pubmed/31237748 http://dx.doi.org/10.1111/jcmm.14509 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Frank, Dorottya Cser, Annamária Kolarovszki, Béla Farkas, Nelli Miseta, Attila Nagy, Tamás Mechanical stress alters protein O‐GlcNAc in human periodontal ligament cells |
title | Mechanical stress alters protein O‐GlcNAc in human periodontal ligament cells |
title_full | Mechanical stress alters protein O‐GlcNAc in human periodontal ligament cells |
title_fullStr | Mechanical stress alters protein O‐GlcNAc in human periodontal ligament cells |
title_full_unstemmed | Mechanical stress alters protein O‐GlcNAc in human periodontal ligament cells |
title_short | Mechanical stress alters protein O‐GlcNAc in human periodontal ligament cells |
title_sort | mechanical stress alters protein o‐glcnac in human periodontal ligament cells |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714205/ https://www.ncbi.nlm.nih.gov/pubmed/31237748 http://dx.doi.org/10.1111/jcmm.14509 |
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