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Long non‐coding RNA H19 is responsible for the progression of lung adenocarcinoma by mediating methylation‐dependent repression of CDH1 promoter

Lung adenocarcinoma is a common histologic type of lung cancer with a high death rate globally. Increasing evidence shows that long non‐coding RNA H19 (lncRNA H19) and CDH1 methylation are involved in multiple tumours. Here, we tried to investigate whether lncRNA H19 or CDH1 methylation could affect...

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Autores principales: Gao, Li‐Ming, Xu, Shu‐Feng, Zheng, Yue, Wang, Ping, Zhang, Ling, Shi, Shan‐Shan, Wu, Tong, Li, Yang, Zhao, Jing, Tian, Qi, Yin, Xiao‐Bo, Zheng, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714219/
https://www.ncbi.nlm.nih.gov/pubmed/31317666
http://dx.doi.org/10.1111/jcmm.14533
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author Gao, Li‐Ming
Xu, Shu‐Feng
Zheng, Yue
Wang, Ping
Zhang, Ling
Shi, Shan‐Shan
Wu, Tong
Li, Yang
Zhao, Jing
Tian, Qi
Yin, Xiao‐Bo
Zheng, Lei
author_facet Gao, Li‐Ming
Xu, Shu‐Feng
Zheng, Yue
Wang, Ping
Zhang, Ling
Shi, Shan‐Shan
Wu, Tong
Li, Yang
Zhao, Jing
Tian, Qi
Yin, Xiao‐Bo
Zheng, Lei
author_sort Gao, Li‐Ming
collection PubMed
description Lung adenocarcinoma is a common histologic type of lung cancer with a high death rate globally. Increasing evidence shows that long non‐coding RNA H19 (lncRNA H19) and CDH1 methylation are involved in multiple tumours. Here, we tried to investigate whether lncRNA H19 or CDH1 methylation could affect the development of lung adenocarcinoma. First, lung adenocarcinoma tissues were collected to detect CDH1 methylation. Then, the regulatory mechanisms of lncRNA H19 were detected mainly in concert with the treatment of overexpression of lncRNA H19, siRNA against lncRNA H19, overexpression of CDH1 and demethylating agent A‐5az in lung adenocarcinoma A549 cell. The expression of lncRNA H19 and epithelial‐mesenchymal transition (EMT)‐related factors as well as cell proliferation, sphere‐forming ability, apoptosis, migration and invasion were detected. Finally, we observed xenograft tumour in nude mice so as to ascertain tumorigenicity of lung adenocarcinoma cells. LncRNA H19 and methylation of CDH1 were highly expressed in lung adenocarcinoma tissues. A549 cells with silencing of lncRNA H19, overexpression of CDH1 or reduced CDH1 methylation by demethylating agent 5‐Az had suppressed cell proliferation, sphere‐forming ability, apoptosis, migration and invasion, in addition to inhibited EMT process. Silencing lncRNA H19 could reduce methylation level of CDH1. In vivo, A549 cells with silencing lncRNA H19, overexpression of CDH1 or reduced CDH1 methylation exhibited low tumorigenicity, reflected by the smaller tumour size and lighter tumour weight. Taken together, this study demonstrates that silencing of lncRNA H19 inhibits EMT and proliferation while promoting apoptosis of lung adenocarcinoma cells by inhibiting methylation of CDH1 promoter.
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spelling pubmed-67142192019-09-05 Long non‐coding RNA H19 is responsible for the progression of lung adenocarcinoma by mediating methylation‐dependent repression of CDH1 promoter Gao, Li‐Ming Xu, Shu‐Feng Zheng, Yue Wang, Ping Zhang, Ling Shi, Shan‐Shan Wu, Tong Li, Yang Zhao, Jing Tian, Qi Yin, Xiao‐Bo Zheng, Lei J Cell Mol Med Original Articles Lung adenocarcinoma is a common histologic type of lung cancer with a high death rate globally. Increasing evidence shows that long non‐coding RNA H19 (lncRNA H19) and CDH1 methylation are involved in multiple tumours. Here, we tried to investigate whether lncRNA H19 or CDH1 methylation could affect the development of lung adenocarcinoma. First, lung adenocarcinoma tissues were collected to detect CDH1 methylation. Then, the regulatory mechanisms of lncRNA H19 were detected mainly in concert with the treatment of overexpression of lncRNA H19, siRNA against lncRNA H19, overexpression of CDH1 and demethylating agent A‐5az in lung adenocarcinoma A549 cell. The expression of lncRNA H19 and epithelial‐mesenchymal transition (EMT)‐related factors as well as cell proliferation, sphere‐forming ability, apoptosis, migration and invasion were detected. Finally, we observed xenograft tumour in nude mice so as to ascertain tumorigenicity of lung adenocarcinoma cells. LncRNA H19 and methylation of CDH1 were highly expressed in lung adenocarcinoma tissues. A549 cells with silencing of lncRNA H19, overexpression of CDH1 or reduced CDH1 methylation by demethylating agent 5‐Az had suppressed cell proliferation, sphere‐forming ability, apoptosis, migration and invasion, in addition to inhibited EMT process. Silencing lncRNA H19 could reduce methylation level of CDH1. In vivo, A549 cells with silencing lncRNA H19, overexpression of CDH1 or reduced CDH1 methylation exhibited low tumorigenicity, reflected by the smaller tumour size and lighter tumour weight. Taken together, this study demonstrates that silencing of lncRNA H19 inhibits EMT and proliferation while promoting apoptosis of lung adenocarcinoma cells by inhibiting methylation of CDH1 promoter. John Wiley and Sons Inc. 2019-07-17 2019-09 /pmc/articles/PMC6714219/ /pubmed/31317666 http://dx.doi.org/10.1111/jcmm.14533 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Gao, Li‐Ming
Xu, Shu‐Feng
Zheng, Yue
Wang, Ping
Zhang, Ling
Shi, Shan‐Shan
Wu, Tong
Li, Yang
Zhao, Jing
Tian, Qi
Yin, Xiao‐Bo
Zheng, Lei
Long non‐coding RNA H19 is responsible for the progression of lung adenocarcinoma by mediating methylation‐dependent repression of CDH1 promoter
title Long non‐coding RNA H19 is responsible for the progression of lung adenocarcinoma by mediating methylation‐dependent repression of CDH1 promoter
title_full Long non‐coding RNA H19 is responsible for the progression of lung adenocarcinoma by mediating methylation‐dependent repression of CDH1 promoter
title_fullStr Long non‐coding RNA H19 is responsible for the progression of lung adenocarcinoma by mediating methylation‐dependent repression of CDH1 promoter
title_full_unstemmed Long non‐coding RNA H19 is responsible for the progression of lung adenocarcinoma by mediating methylation‐dependent repression of CDH1 promoter
title_short Long non‐coding RNA H19 is responsible for the progression of lung adenocarcinoma by mediating methylation‐dependent repression of CDH1 promoter
title_sort long non‐coding rna h19 is responsible for the progression of lung adenocarcinoma by mediating methylation‐dependent repression of cdh1 promoter
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714219/
https://www.ncbi.nlm.nih.gov/pubmed/31317666
http://dx.doi.org/10.1111/jcmm.14533
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