One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets

[Image: see text] Enzyme immobilization is an essential prerequisite for biocatalysis. In this context, emulsion provides an excellent template for assembling enzymes at the oil–water interface. A microfluidic approach has been adopted to produce oil-in-water-type emulsions stabilized by gold nanopa...

Descripción completa

Detalles Bibliográficos
Autores principales: Varshney, Rohit, Sharma, Shubhra, Prakash, Bhanu, Laha, Joydev K., Patra, Debabrata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714293/
https://www.ncbi.nlm.nih.gov/pubmed/31497697
http://dx.doi.org/10.1021/acsomega.9b01321
_version_ 1783447034818199552
author Varshney, Rohit
Sharma, Shubhra
Prakash, Bhanu
Laha, Joydev K.
Patra, Debabrata
author_facet Varshney, Rohit
Sharma, Shubhra
Prakash, Bhanu
Laha, Joydev K.
Patra, Debabrata
author_sort Varshney, Rohit
collection PubMed
description [Image: see text] Enzyme immobilization is an essential prerequisite for biocatalysis. In this context, emulsion provides an excellent template for assembling enzymes at the oil–water interface. A microfluidic approach has been adopted to produce oil-in-water-type emulsions stabilized by gold nanoparticle–catalase conjugates. In situ ring-opening polymerization of the oil phase produces solid core enzyme-immobilized microcapsules (MCs). These resultant MCs exhibited a K(m) value of 42 mM and shows 1.1-fold higher activity compared to free enzymes. Finally, the robust MCs showed excellent recyclability, which can meet the demand of industrial biotechnological applications.
format Online
Article
Text
id pubmed-6714293
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-67142932019-09-06 One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets Varshney, Rohit Sharma, Shubhra Prakash, Bhanu Laha, Joydev K. Patra, Debabrata ACS Omega [Image: see text] Enzyme immobilization is an essential prerequisite for biocatalysis. In this context, emulsion provides an excellent template for assembling enzymes at the oil–water interface. A microfluidic approach has been adopted to produce oil-in-water-type emulsions stabilized by gold nanoparticle–catalase conjugates. In situ ring-opening polymerization of the oil phase produces solid core enzyme-immobilized microcapsules (MCs). These resultant MCs exhibited a K(m) value of 42 mM and shows 1.1-fold higher activity compared to free enzymes. Finally, the robust MCs showed excellent recyclability, which can meet the demand of industrial biotechnological applications. American Chemical Society 2019-08-12 /pmc/articles/PMC6714293/ /pubmed/31497697 http://dx.doi.org/10.1021/acsomega.9b01321 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Varshney, Rohit
Sharma, Shubhra
Prakash, Bhanu
Laha, Joydev K.
Patra, Debabrata
One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets
title One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets
title_full One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets
title_fullStr One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets
title_full_unstemmed One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets
title_short One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets
title_sort one-step fabrication of enzyme-immobilized reusable polymerized microcapsules from microfluidic droplets
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714293/
https://www.ncbi.nlm.nih.gov/pubmed/31497697
http://dx.doi.org/10.1021/acsomega.9b01321
work_keys_str_mv AT varshneyrohit onestepfabricationofenzymeimmobilizedreusablepolymerizedmicrocapsulesfrommicrofluidicdroplets
AT sharmashubhra onestepfabricationofenzymeimmobilizedreusablepolymerizedmicrocapsulesfrommicrofluidicdroplets
AT prakashbhanu onestepfabricationofenzymeimmobilizedreusablepolymerizedmicrocapsulesfrommicrofluidicdroplets
AT lahajoydevk onestepfabricationofenzymeimmobilizedreusablepolymerizedmicrocapsulesfrommicrofluidicdroplets
AT patradebabrata onestepfabricationofenzymeimmobilizedreusablepolymerizedmicrocapsulesfrommicrofluidicdroplets

Ejemplares similares