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An animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence

INTRODUCTION: Minimally invasive methods for injured ligament and tendon restoration have been developed and gained popularity in recent years. Injury and relaxation of the pubourethral ligament (PUL) can lead to stress urinary incontinence (SUI). The aim of this study was to investigate the impact...

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Autores principales: Nikolopoulos, Kostis I., Chrysanthopoulou, Eleftheria, Pergialiotis, Vasilios, Korrou, Laskarina Maria, Perrea, Despina N., Dimitroulis, Dimitrios, Doumouchtsis, Stergios K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Polish Urological Association 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715089/
https://www.ncbi.nlm.nih.gov/pubmed/31482019
http://dx.doi.org/10.5173/ceju.2019.1896
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author Nikolopoulos, Kostis I.
Chrysanthopoulou, Eleftheria
Pergialiotis, Vasilios
Korrou, Laskarina Maria
Perrea, Despina N.
Dimitroulis, Dimitrios
Doumouchtsis, Stergios K.
author_facet Nikolopoulos, Kostis I.
Chrysanthopoulou, Eleftheria
Pergialiotis, Vasilios
Korrou, Laskarina Maria
Perrea, Despina N.
Dimitroulis, Dimitrios
Doumouchtsis, Stergios K.
author_sort Nikolopoulos, Kostis I.
collection PubMed
description INTRODUCTION: Minimally invasive methods for injured ligament and tendon restoration have been developed and gained popularity in recent years. Injury and relaxation of the pubourethral ligament (PUL) can lead to stress urinary incontinence (SUI). The aim of this study was to investigate the impact of injecting platelet rich plasma (PRP) into the PUL following its surgical transection resulting in SUI, confirmed by leak point pressure (LPP) measurements pre- and post-intervention in an experimental animal model. MATERIAL AND METHODS: Twenty female adult Sprague-Dawley rats were assigned in 2 groups: 1) treatment group with transection of the PUL and application of PRP at the time of transection and at one month follow-up and 2) a control group, with transection of the PUL only. Leak point pressures (LPPs) were measured prior to transection, immediately following the transection and at 1 and 2 months in both groups. RESULTS: The median LPPs for the control group were: LPP – preT: 35.6 (29.8–44.8) cmH(2)O, LPP – postT: 14.6 (5.8–19.0) cmH(2)O, LPP – 1 month: 27.3 (19.2–33.8) cmH(2)O, LPP – 2 months: 29.0 (27.0–34.0) cmH(2)O, whereas for the PRP group were: LPP-preT: 40.5 (33.2–46.3) cmH(2)O, LPP – postT: 15.7 (3.0–24.0) cmH(2)O, LPP – 1month: 31.6 (24.8–37.4) cmH(2)O, LPP – 2 months: 36.8 (32.5–45.4) cmH(2)O. PRP injections on transected PULs significantly increased LPPs at one month follow-up [31.6 cmH(2)O vs. 27.3 cmH(2)O, p = .043]. This effect was confirmed at two months [36.8 cmH(2)O vs. 29.0 cmH(2)O, p <.001]. CONCLUSIONS: Injection of PRP into transected PULs significantly improved LPPs at one and two months' follow-up. However, further experimental and clinical research is needed to evaluate the safety and efficacy of this treatment, in clinical practice.
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spelling pubmed-67150892019-09-03 An animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence Nikolopoulos, Kostis I. Chrysanthopoulou, Eleftheria Pergialiotis, Vasilios Korrou, Laskarina Maria Perrea, Despina N. Dimitroulis, Dimitrios Doumouchtsis, Stergios K. Cent European J Urol Original Paper INTRODUCTION: Minimally invasive methods for injured ligament and tendon restoration have been developed and gained popularity in recent years. Injury and relaxation of the pubourethral ligament (PUL) can lead to stress urinary incontinence (SUI). The aim of this study was to investigate the impact of injecting platelet rich plasma (PRP) into the PUL following its surgical transection resulting in SUI, confirmed by leak point pressure (LPP) measurements pre- and post-intervention in an experimental animal model. MATERIAL AND METHODS: Twenty female adult Sprague-Dawley rats were assigned in 2 groups: 1) treatment group with transection of the PUL and application of PRP at the time of transection and at one month follow-up and 2) a control group, with transection of the PUL only. Leak point pressures (LPPs) were measured prior to transection, immediately following the transection and at 1 and 2 months in both groups. RESULTS: The median LPPs for the control group were: LPP – preT: 35.6 (29.8–44.8) cmH(2)O, LPP – postT: 14.6 (5.8–19.0) cmH(2)O, LPP – 1 month: 27.3 (19.2–33.8) cmH(2)O, LPP – 2 months: 29.0 (27.0–34.0) cmH(2)O, whereas for the PRP group were: LPP-preT: 40.5 (33.2–46.3) cmH(2)O, LPP – postT: 15.7 (3.0–24.0) cmH(2)O, LPP – 1month: 31.6 (24.8–37.4) cmH(2)O, LPP – 2 months: 36.8 (32.5–45.4) cmH(2)O. PRP injections on transected PULs significantly increased LPPs at one month follow-up [31.6 cmH(2)O vs. 27.3 cmH(2)O, p = .043]. This effect was confirmed at two months [36.8 cmH(2)O vs. 29.0 cmH(2)O, p <.001]. CONCLUSIONS: Injection of PRP into transected PULs significantly improved LPPs at one and two months' follow-up. However, further experimental and clinical research is needed to evaluate the safety and efficacy of this treatment, in clinical practice. Polish Urological Association 2019-06-04 2019 /pmc/articles/PMC6715089/ /pubmed/31482019 http://dx.doi.org/10.5173/ceju.2019.1896 Text en Copyright by Polish Urological Association http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Original Paper
Nikolopoulos, Kostis I.
Chrysanthopoulou, Eleftheria
Pergialiotis, Vasilios
Korrou, Laskarina Maria
Perrea, Despina N.
Dimitroulis, Dimitrios
Doumouchtsis, Stergios K.
An animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence
title An animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence
title_full An animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence
title_fullStr An animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence
title_full_unstemmed An animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence
title_short An animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence
title_sort animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715089/
https://www.ncbi.nlm.nih.gov/pubmed/31482019
http://dx.doi.org/10.5173/ceju.2019.1896
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