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EXTL3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells

Endometriosis is a benign chronic condition characterized by the existence of endometrial-like stroma and glandular tissue in extrauterine locations. The molecular mechanisms of its pathogenesis have not been elucidated. We have studied the role of EXTL3 (exostosin-like 3) in endometriosis and found...

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Autores principales: Aints, Alar, Mölder, Signe, Salumets, Andres
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715673/
https://www.ncbi.nlm.nih.gov/pubmed/31467315
http://dx.doi.org/10.1038/s41598-019-48840-8
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author Aints, Alar
Mölder, Signe
Salumets, Andres
author_facet Aints, Alar
Mölder, Signe
Salumets, Andres
author_sort Aints, Alar
collection PubMed
description Endometriosis is a benign chronic condition characterized by the existence of endometrial-like stroma and glandular tissue in extrauterine locations. The molecular mechanisms of its pathogenesis have not been elucidated. We have studied the role of EXTL3 (exostosin-like 3) in endometriosis and found that it is expressed in endometrial tissue as well as endometriosis lesions. We have found that serum from endometriosis patients contains a factor or factors, which interact with EXTL3 resulting in strongly increased colony formation in regenerating cell culture. We also found increased anti-EXTL3 antibodies in endometriosis patients’ sera. EXTL3 is an N-acetyl glucosamine (GlcNAc) transferase, performing a key step in heparan sulfate (HS) glucosaminoglycan synthesis. Many viruses replicate in regenerating epithelial cells and use HS as a receptor for cell entry. We measured antibody titres to viruses, which use HS as a receptor for cell entry, and found rarely increased titres for these viruses in endometriosis sera, whereas titres to viruses using other receptors were equally distributed in study groups. The data indicate that perturbation of HS metabolism is associated with endometriosis.
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spelling pubmed-67156732019-09-13 EXTL3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells Aints, Alar Mölder, Signe Salumets, Andres Sci Rep Article Endometriosis is a benign chronic condition characterized by the existence of endometrial-like stroma and glandular tissue in extrauterine locations. The molecular mechanisms of its pathogenesis have not been elucidated. We have studied the role of EXTL3 (exostosin-like 3) in endometriosis and found that it is expressed in endometrial tissue as well as endometriosis lesions. We have found that serum from endometriosis patients contains a factor or factors, which interact with EXTL3 resulting in strongly increased colony formation in regenerating cell culture. We also found increased anti-EXTL3 antibodies in endometriosis patients’ sera. EXTL3 is an N-acetyl glucosamine (GlcNAc) transferase, performing a key step in heparan sulfate (HS) glucosaminoglycan synthesis. Many viruses replicate in regenerating epithelial cells and use HS as a receptor for cell entry. We measured antibody titres to viruses, which use HS as a receptor for cell entry, and found rarely increased titres for these viruses in endometriosis sera, whereas titres to viruses using other receptors were equally distributed in study groups. The data indicate that perturbation of HS metabolism is associated with endometriosis. Nature Publishing Group UK 2019-08-29 /pmc/articles/PMC6715673/ /pubmed/31467315 http://dx.doi.org/10.1038/s41598-019-48840-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Aints, Alar
Mölder, Signe
Salumets, Andres
EXTL3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells
title EXTL3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells
title_full EXTL3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells
title_fullStr EXTL3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells
title_full_unstemmed EXTL3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells
title_short EXTL3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells
title_sort extl3-interacting endometriosis-specific serum factors induce colony formation of endometrial stromal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715673/
https://www.ncbi.nlm.nih.gov/pubmed/31467315
http://dx.doi.org/10.1038/s41598-019-48840-8
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