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Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig
Blood circulation mainly aims at distributing the nutrients required for tissue metabolism and collecting safely the by-products of all tissues to be further metabolized or eliminated. The simultaneous study of arterial (A) and venous (V) specific metabolites therefore has appeared to be a more rele...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715693/ https://www.ncbi.nlm.nih.gov/pubmed/31467335 http://dx.doi.org/10.1038/s41598-019-48997-2 |
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author | Poupin, Nathalie Tremblay-Franco, Marie Amiel, Aurélien Canlet, Cécile Rémond, Didier Debrauwer, Laurent Dardevet, Dominique Thiele, Ines Aurich, Maike K. Jourdan, Fabien Savary-Auzeloux, Isabelle Polakof, Sergio |
author_facet | Poupin, Nathalie Tremblay-Franco, Marie Amiel, Aurélien Canlet, Cécile Rémond, Didier Debrauwer, Laurent Dardevet, Dominique Thiele, Ines Aurich, Maike K. Jourdan, Fabien Savary-Auzeloux, Isabelle Polakof, Sergio |
author_sort | Poupin, Nathalie |
collection | PubMed |
description | Blood circulation mainly aims at distributing the nutrients required for tissue metabolism and collecting safely the by-products of all tissues to be further metabolized or eliminated. The simultaneous study of arterial (A) and venous (V) specific metabolites therefore has appeared to be a more relevant approach to understand and study the metabolism of a given organ. We propose to implement this approach by applying a metabolomics (NMR) strategy on paired AV blood across the intestine and liver on high fat/high sugar (HFHS)-fed minipigs. Our objective was to unravel kinetically and sequentially the metabolic adaptations to early obesity/insulin resistance onset specifically on these two tissues. After two months of HFHS feeding our study of AV ratios of the metabolome highlighted three major features. First, the hepatic metabolism switched from carbohydrate to lipid utilization. Second, the energy demand of the intestine increased, resulting in an enhanced uptake of glutamine, glutamate, and the recruitment of novel energy substrates (choline and creatine). Third, the uptake of methionine and threonine was considered to be driven by an increased intestine turnover to cope with the new high-density diet. Finally, the unique combination of experimental data and modelling predictions suggested that HFHS feeding was associated with changes in tryptophan metabolism and fatty acid β-oxidation, which may play an important role in lipid hepatic accumulation and insulin sensitivity. |
format | Online Article Text |
id | pubmed-6715693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67156932019-09-13 Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig Poupin, Nathalie Tremblay-Franco, Marie Amiel, Aurélien Canlet, Cécile Rémond, Didier Debrauwer, Laurent Dardevet, Dominique Thiele, Ines Aurich, Maike K. Jourdan, Fabien Savary-Auzeloux, Isabelle Polakof, Sergio Sci Rep Article Blood circulation mainly aims at distributing the nutrients required for tissue metabolism and collecting safely the by-products of all tissues to be further metabolized or eliminated. The simultaneous study of arterial (A) and venous (V) specific metabolites therefore has appeared to be a more relevant approach to understand and study the metabolism of a given organ. We propose to implement this approach by applying a metabolomics (NMR) strategy on paired AV blood across the intestine and liver on high fat/high sugar (HFHS)-fed minipigs. Our objective was to unravel kinetically and sequentially the metabolic adaptations to early obesity/insulin resistance onset specifically on these two tissues. After two months of HFHS feeding our study of AV ratios of the metabolome highlighted three major features. First, the hepatic metabolism switched from carbohydrate to lipid utilization. Second, the energy demand of the intestine increased, resulting in an enhanced uptake of glutamine, glutamate, and the recruitment of novel energy substrates (choline and creatine). Third, the uptake of methionine and threonine was considered to be driven by an increased intestine turnover to cope with the new high-density diet. Finally, the unique combination of experimental data and modelling predictions suggested that HFHS feeding was associated with changes in tryptophan metabolism and fatty acid β-oxidation, which may play an important role in lipid hepatic accumulation and insulin sensitivity. Nature Publishing Group UK 2019-08-29 /pmc/articles/PMC6715693/ /pubmed/31467335 http://dx.doi.org/10.1038/s41598-019-48997-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Poupin, Nathalie Tremblay-Franco, Marie Amiel, Aurélien Canlet, Cécile Rémond, Didier Debrauwer, Laurent Dardevet, Dominique Thiele, Ines Aurich, Maike K. Jourdan, Fabien Savary-Auzeloux, Isabelle Polakof, Sergio Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig |
title | Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig |
title_full | Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig |
title_fullStr | Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig |
title_full_unstemmed | Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig |
title_short | Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig |
title_sort | arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese yucatan minipig |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715693/ https://www.ncbi.nlm.nih.gov/pubmed/31467335 http://dx.doi.org/10.1038/s41598-019-48997-2 |
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