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Key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the Escherichia coli ST410 lineage

There is an urgent need to understand the global epidemiological landscape of carbapenem-resistant Escherichia coli (CREC). Here we provide combined genomic and phenotypic characterization of the emergence of a CREC clone from the ST410 lineage. We show that the clone expands with a single plasmid,...

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Autores principales: Feng, Yu, Liu, Lu, Lin, Ji, Ma, Ke, Long, Haiyan, Wei, Li, Xie, Yi, McNally, Alan, Zong, Zhiyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715731/
https://www.ncbi.nlm.nih.gov/pubmed/31482141
http://dx.doi.org/10.1038/s42003-019-0569-1
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author Feng, Yu
Liu, Lu
Lin, Ji
Ma, Ke
Long, Haiyan
Wei, Li
Xie, Yi
McNally, Alan
Zong, Zhiyong
author_facet Feng, Yu
Liu, Lu
Lin, Ji
Ma, Ke
Long, Haiyan
Wei, Li
Xie, Yi
McNally, Alan
Zong, Zhiyong
author_sort Feng, Yu
collection PubMed
description There is an urgent need to understand the global epidemiological landscape of carbapenem-resistant Escherichia coli (CREC). Here we provide combined genomic and phenotypic characterization of the emergence of a CREC clone from the ST410 lineage. We show that the clone expands with a single plasmid, within which there is frequent switching of the carbapenemase gene type between bla(NDM) and bla(OXA-181) with no impact on plasmid stability or fitness. A search for clone-specific traits identified unique alleles of genes involved in adhesion and iron acquisition, which have been imported via recombination. These recombination-derived allelic switches had no impact on virulence in a simple infection model, but decreased efficiency in binding to abiotic surfaces and greatly enhanced fitness in iron limited conditions. Together our data show a footprint for evolution of a CREC clone, whereby recombination drives new alleles into the clone which provide a competitive advantage in colonizing mammalian hosts.
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spelling pubmed-67157312019-09-03 Key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the Escherichia coli ST410 lineage Feng, Yu Liu, Lu Lin, Ji Ma, Ke Long, Haiyan Wei, Li Xie, Yi McNally, Alan Zong, Zhiyong Commun Biol Article There is an urgent need to understand the global epidemiological landscape of carbapenem-resistant Escherichia coli (CREC). Here we provide combined genomic and phenotypic characterization of the emergence of a CREC clone from the ST410 lineage. We show that the clone expands with a single plasmid, within which there is frequent switching of the carbapenemase gene type between bla(NDM) and bla(OXA-181) with no impact on plasmid stability or fitness. A search for clone-specific traits identified unique alleles of genes involved in adhesion and iron acquisition, which have been imported via recombination. These recombination-derived allelic switches had no impact on virulence in a simple infection model, but decreased efficiency in binding to abiotic surfaces and greatly enhanced fitness in iron limited conditions. Together our data show a footprint for evolution of a CREC clone, whereby recombination drives new alleles into the clone which provide a competitive advantage in colonizing mammalian hosts. Nature Publishing Group UK 2019-08-29 /pmc/articles/PMC6715731/ /pubmed/31482141 http://dx.doi.org/10.1038/s42003-019-0569-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Feng, Yu
Liu, Lu
Lin, Ji
Ma, Ke
Long, Haiyan
Wei, Li
Xie, Yi
McNally, Alan
Zong, Zhiyong
Key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the Escherichia coli ST410 lineage
title Key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the Escherichia coli ST410 lineage
title_full Key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the Escherichia coli ST410 lineage
title_fullStr Key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the Escherichia coli ST410 lineage
title_full_unstemmed Key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the Escherichia coli ST410 lineage
title_short Key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the Escherichia coli ST410 lineage
title_sort key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the escherichia coli st410 lineage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715731/
https://www.ncbi.nlm.nih.gov/pubmed/31482141
http://dx.doi.org/10.1038/s42003-019-0569-1
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