Cargando…

Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis

Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation. The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO(®) vecto...

Descripción completa

Detalles Bibliográficos
Autores principales:	Silva, Felipe D., Oliveira, João E., Freire, Renan P., Suzuki, Miriam F., Soares, Carlos R., Bartolini, Paolo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2019
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715758/ https://www.ncbi.nlm.nih.gov/pubmed/31468229 http://dx.doi.org/10.1186/s13568-019-0856-8

Ejemplares similares

HEK293T cell lines defective for O-linked glycosylation
por: Termini, James M., et al.
Publicado: (2017)

Exploring Site-Specific N-Glycosylation of HEK293 and Plant-Produced Human IgA Isotypes
por: Göritzer, Kathrin, et al.
Publicado: (2017)

Periplasmic synthesis and purification of the human prolactin antagonist Δ(1-11)-G129R-hPRL
por: Suzuki, Miriam F., et al.
Publicado: (2021)

Installation of O-glycan sulfation capacities in human HEK293 cells for display of sulfated mucins
por: Sun, Lingbo, et al.
Publicado: (2021)

Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells
por: Böhm, Ernst, et al.
Publicado: (2015)

Homogeneously N-glycosylated proteins derived from the GlycoDelete HEK293 cell line enable diffraction-quality crystallogenesis
por: Kozak, Sandra, et al.
Publicado: (2020)

Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells
por: Loignon, Martin, et al.
Publicado: (2008)

Exploring the glycosylation of mucins by use of O-glycodomain reporters recombinantly expressed in glycoengineered HEK293 cells
por: Konstantinidi, Andriana, et al.
Publicado: (2022)

Expression and characterization of mouse prolactin (mPRL) in CHO dhfr- cells
por: Suzuki, Miriam Fussae, et al.
Publicado: (2014)

Proteomic Landscape of Adeno-Associated Virus (AAV)-Producing HEK293 Cells
por: Strasser, Lisa, et al.
Publicado: (2021)

Thyrotropin N-glycosylation and Glycan Composition in Severe Primary Hypothyroidism
por: Wide, Leif, et al.
Publicado: (2021)

HEK293S Cells Have Functional Retinoid Processing Machinery
por: Brueggemann, Lioubov I., et al.
Publicado: (2002)

Effects of endotoxin on pacemaker funny current in HEK 293 cells
por: Papaioannou, VP, et al.
Publicado: (2011)

Echinacea-induced cytosolic Ca(2+ )elevation in HEK293
por: Wu, Lankun, et al.
Publicado: (2010)

Effect of PTTG on endogenous gene expression in HEK 293 cells
por: Panguluri, Siva K, et al.
Publicado: (2009)

Analysis of DNA topology of EBV minichromosomes in HEK 293 cells
por: Castán, Alicia, et al.
Publicado: (2017)

Microtubule Integrity Is Associated with the Functional Activity of Mitochondria in HEK293
por: Cho, Min Jeong, et al.
Publicado: (2021)

CD133 induces tumour-initiating properties in HEK293 cells
por: Canis, Martin, et al.
Publicado: (2012)

Differences in the production of hyperglycosylated IFN alpha in CHO and HEK 293 cells
por: Gugliotta, Agustina, et al.
Publicado: (2013)

Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells
por: Selvaraj, Vellaisamy, et al.
Publicado: (2014)

Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature
por: Lin, Chi-Yen, et al.
Publicado: (2015)

Cellular signaling pathways modulated by influenza infection in HEK293 cells
por: Manceur, Aziza P, et al.
Publicado: (2015)

HEK293T Cells Are Heterozygous for CCR5 Delta 32 Mutation
por: Qi, Chunxia, et al.
Publicado: (2016)

Distinct mechanical behavior of HEK293 cells in adherent and suspended states
por: Haghparast, Seyed Mohammad Ali, et al.
Publicado: (2015)

Using HEK293T Expression System to Study Photoactive Plant Cryptochromes
por: Yang, Liang, et al.
Publicado: (2016)

Lack of current observed in HEK293 cells expressing NALCN channels
por: Egan, Jennifer M., et al.
Publicado: (2018)

Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells
por: Blessing, Daniel, et al.
Publicado: (2018)

Degradation of Extracellular NAD(+) Intermediates in Cultures of Human HEK293 Cells
por: Kulikova, Veronika, et al.
Publicado: (2019)

Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells
por: Oladzad, Azarakhsh, et al.
Publicado: (2020)

Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
por: MITOMA, Shuya, et al.
Publicado: (2020)

N-Glycoprofiling Analysis for Carbohydrate Composition and Site-Occupancy Determination in a Poly-Glycosylated Protein: Human Thyrotropin of Different Origins
por: Ribela, Maria Teresa C. P., et al.
Publicado: (2017)

Extracellular acidification induces ROS- and mPTP-mediated death in HEK293 cells
por: Teixeira, José, et al.
Publicado: (2017)

Metabolic and Kinetic analyses of influenza production in perfusion HEK293 cell culture
por: Petiot, Emma, et al.
Publicado: (2011)

Characterization of crude Echis carinatus venom-induced cytotoxicity in HEK 293T cells
por: Pierce, Rebecca D, et al.
Publicado: (2011)

A Multi-Omics Analysis of Recombinant Protein Production in Hek293 Cells
por: Dietmair, Stefanie, et al.
Publicado: (2012)

Cholesterol Down-Regulates BK Channels Stably Expressed in HEK 293 Cells
por: Wu, Wei, et al.
Publicado: (2013)

Analysis of Wnt signaling β-catenin spatial dynamics in HEK293T cells
por: Tan, Chin Wee, et al.
Publicado: (2014)

Overexpression of Mitochondrial Sirtuins Alters Glycolysis and Mitochondrial Function in HEK293 Cells
por: Barbi de Moura, Michelle, et al.
Publicado: (2014)

Adaptation to serum-free culture of HEK 293T and Huh7.0 cells
por: Beltran Paschoal, Juliana Fontes, et al.
Publicado: (2014)

Small RNA Analysis in Sindbis Virus Infected Human HEK293 Cells
por: Donaszi-Ivanov, Andras, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_3jsd6ad6oi401b8jqet5s09526