Development and validation of a reliable LC–MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis

OBJECTIVE: The purpose of this study was to develop and validate a specific and sensitive liquid chromatography tandem mass-spectrometry method for quantification of usnic acid concentration in the lichen, Cladonia uncialis, suitable for detection of relatively small fluctuations of usnic acid conce...

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Detalles Bibliográficos
Autores principales: Sveshnikova, Natalia, Yuan, Tao, Warren, Jamie M., Piercey-Normore, Michele D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716858/
https://www.ncbi.nlm.nih.gov/pubmed/31470895
http://dx.doi.org/10.1186/s13104-019-4580-x
Descripción
Sumario:OBJECTIVE: The purpose of this study was to develop and validate a specific and sensitive liquid chromatography tandem mass-spectrometry method for quantification of usnic acid concentration in the lichen, Cladonia uncialis, suitable for detection of relatively small fluctuations of usnic acid concentration in response to environmental changes. RESULTS: The resulting method was fully validated according to international guidelines and demonstrated good selectivity and sensitivity with minor levels of a matrix effect and high accuracy.

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