Development and validation of a reliable LC–MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis

OBJECTIVE: The purpose of this study was to develop and validate a specific and sensitive liquid chromatography tandem mass-spectrometry method for quantification of usnic acid concentration in the lichen, Cladonia uncialis, suitable for detection of relatively small fluctuations of usnic acid conce...

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Autores principales: Sveshnikova, Natalia, Yuan, Tao, Warren, Jamie M., Piercey-Normore, Michele D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716858/
https://www.ncbi.nlm.nih.gov/pubmed/31470895
http://dx.doi.org/10.1186/s13104-019-4580-x
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author Sveshnikova, Natalia
Yuan, Tao
Warren, Jamie M.
Piercey-Normore, Michele D.
author_facet Sveshnikova, Natalia
Yuan, Tao
Warren, Jamie M.
Piercey-Normore, Michele D.
author_sort Sveshnikova, Natalia
collection PubMed
description OBJECTIVE: The purpose of this study was to develop and validate a specific and sensitive liquid chromatography tandem mass-spectrometry method for quantification of usnic acid concentration in the lichen, Cladonia uncialis, suitable for detection of relatively small fluctuations of usnic acid concentration in response to environmental changes. RESULTS: The resulting method was fully validated according to international guidelines and demonstrated good selectivity and sensitivity with minor levels of a matrix effect and high accuracy.
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spelling pubmed-67168582019-09-04 Development and validation of a reliable LC–MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis Sveshnikova, Natalia Yuan, Tao Warren, Jamie M. Piercey-Normore, Michele D. BMC Res Notes Research Note OBJECTIVE: The purpose of this study was to develop and validate a specific and sensitive liquid chromatography tandem mass-spectrometry method for quantification of usnic acid concentration in the lichen, Cladonia uncialis, suitable for detection of relatively small fluctuations of usnic acid concentration in response to environmental changes. RESULTS: The resulting method was fully validated according to international guidelines and demonstrated good selectivity and sensitivity with minor levels of a matrix effect and high accuracy. BioMed Central 2019-08-30 /pmc/articles/PMC6716858/ /pubmed/31470895 http://dx.doi.org/10.1186/s13104-019-4580-x Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Sveshnikova, Natalia
Yuan, Tao
Warren, Jamie M.
Piercey-Normore, Michele D.
Development and validation of a reliable LC–MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis
title Development and validation of a reliable LC–MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis
title_full Development and validation of a reliable LC–MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis
title_fullStr Development and validation of a reliable LC–MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis
title_full_unstemmed Development and validation of a reliable LC–MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis
title_short Development and validation of a reliable LC–MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis
title_sort development and validation of a reliable lc–ms/ms method for quantitative analysis of usnic acid in cladonia uncialis
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716858/
https://www.ncbi.nlm.nih.gov/pubmed/31470895
http://dx.doi.org/10.1186/s13104-019-4580-x
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