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Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field

BACKGROUND: Microtubule proteins are able to produce electromagnetic fields and have an important role in memory formation, and learning. Therefore, microtubules have the potential to be affected by exogenous electromagnetic fields. This study aimed to examine the comparison of microtubule polymeriz...

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Autores principales: Gholami, Dariush, Riazi, Gholamhossein, Fathi, Rouhollah, Sharafi, Mohsen, Shahverdi, Abdolhossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716927/
https://www.ncbi.nlm.nih.gov/pubmed/31464580
http://dx.doi.org/10.1186/s12860-019-0224-1
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author Gholami, Dariush
Riazi, Gholamhossein
Fathi, Rouhollah
Sharafi, Mohsen
Shahverdi, Abdolhossein
author_facet Gholami, Dariush
Riazi, Gholamhossein
Fathi, Rouhollah
Sharafi, Mohsen
Shahverdi, Abdolhossein
author_sort Gholami, Dariush
collection PubMed
description BACKGROUND: Microtubule proteins are able to produce electromagnetic fields and have an important role in memory formation, and learning. Therefore, microtubules have the potential to be affected by exogenous electromagnetic fields. This study aimed to examine the comparison of microtubule polymerization and its structural behavior in brain and sperm affected by 50 Hz extremely low-frequency electromagnetic field (ELEF). RESULTS: Twenties adult male rats were randomly and equally divided into control and experimental groups, to evaluate the effect of 50 Hz ELEF on the sperm and brain functions. Plus-maze, serum testosterone and corticosterone, and sperm evaluation were performed. Next, the semen and brain samples were obtained, and they were divided into four experimental groups for investigation of microtubule polymerization. There was no significant difference in testosterone and, corticosterone levels, anxiety behaviors, and sperm morphology between control and ELEF-exposure groups. The sperm viability, total and progressive motility were significantly higher in the ELEF-exposed group than that of the control group. The microtubule polymerization in sperm ELEF was significantly higher than in other groups. The secondary and tertiary structures of tubulins were significantly affected in the brain, and sperm ELEF groups. CONCLUSION: It seems that the polymerization of microtubules and conformational changes of tubulin dimers are improved by ELEF application.
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spelling pubmed-67169272019-09-05 Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field Gholami, Dariush Riazi, Gholamhossein Fathi, Rouhollah Sharafi, Mohsen Shahverdi, Abdolhossein BMC Mol Cell Biol Research Article BACKGROUND: Microtubule proteins are able to produce electromagnetic fields and have an important role in memory formation, and learning. Therefore, microtubules have the potential to be affected by exogenous electromagnetic fields. This study aimed to examine the comparison of microtubule polymerization and its structural behavior in brain and sperm affected by 50 Hz extremely low-frequency electromagnetic field (ELEF). RESULTS: Twenties adult male rats were randomly and equally divided into control and experimental groups, to evaluate the effect of 50 Hz ELEF on the sperm and brain functions. Plus-maze, serum testosterone and corticosterone, and sperm evaluation were performed. Next, the semen and brain samples were obtained, and they were divided into four experimental groups for investigation of microtubule polymerization. There was no significant difference in testosterone and, corticosterone levels, anxiety behaviors, and sperm morphology between control and ELEF-exposure groups. The sperm viability, total and progressive motility were significantly higher in the ELEF-exposed group than that of the control group. The microtubule polymerization in sperm ELEF was significantly higher than in other groups. The secondary and tertiary structures of tubulins were significantly affected in the brain, and sperm ELEF groups. CONCLUSION: It seems that the polymerization of microtubules and conformational changes of tubulin dimers are improved by ELEF application. BioMed Central 2019-08-29 /pmc/articles/PMC6716927/ /pubmed/31464580 http://dx.doi.org/10.1186/s12860-019-0224-1 Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Gholami, Dariush
Riazi, Gholamhossein
Fathi, Rouhollah
Sharafi, Mohsen
Shahverdi, Abdolhossein
Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field
title Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field
title_full Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field
title_fullStr Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field
title_full_unstemmed Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field
title_short Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field
title_sort comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716927/
https://www.ncbi.nlm.nih.gov/pubmed/31464580
http://dx.doi.org/10.1186/s12860-019-0224-1
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